Tissue engineering tracheal patch culture method and culture system

A technology of tissue engineering and culture method, applied in the field of biomedicine, can solve problems such as granulation tissue hyperplasia, tissue structure differences, inability to exert tracheal physiological and immune functions, etc., and achieve the effect of improving living conditions and restoring physiological functions

Inactive Publication Date: 2018-08-24
SHANGHAI PULMONARY HOSPITAL
View PDF9 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing trachea substitutes have the following technical problems: (1) The biocompatibility of the artificial trachea is poor, and granulation tissue hyperplasia is prone to occur, and the airway epithelium cannot be attached, so it is diff...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue engineering tracheal patch culture method and culture system
  • Tissue engineering tracheal patch culture method and culture system
  • Tissue engineering tracheal patch culture method and culture system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] According to the location, diameter, and thickness of the damaged trachea suggested by the patient's CT image and tracheal endoscopy, a tracheal patch of appropriate shape and size is designed. Using 3D printing technology, according to the designed size, a culture system for tissue engineered tracheal patches of appropriate size is printed out. This system has the following characteristics:

[0040] Such as figure 1 As shown, the system consists of a three-layer structure consisting of a bottom hard material layer 10 of a cell culture dish-like hard material, a middle cell layer 20 of a chondrocyte hydrogel, and a top membrane layer 30 of an epithelial cell membrane.

[0041] Such as Figure 2-3 As shown, the bottom hard material layer 10 is made by 3D printing, and the hard material of polylactic acid-caprolactone copolymer material (PLLACL) is used to print into the shape of a petri dish, and the bottom is made by 3D printing. The inlet 12 and the outlet 11 of the ...

Embodiment 2

[0046] Patient XX underwent tracheal side wall resection due to airway lesions, and needed to transplant tracheal substitutes for airway reconstruction. The tissue-engineered tracheal patch culture method constructed by the 3D printing composite tissue cell layered culture system was used to obtain tissue-engineered tracheal substitutes. Specifically include:

[0047] (1) Obtain the patient's autologous cartilage and epithelial cells through bronchoscope biopsy before operation, and culture them in vitro;

[0048] (2) if figure 1 As shown, the dish-shaped bottom hard material layer 10 with a flow channel system is 3D printed using PLLACL material;

[0049](3) suspending the cultured and proliferated chondrocytes with the hydrogel, and then printing the hydrogel chondrocytes above the dish-shaped bottom hard material layer 10 to form the middle cell layer 20;

[0050] (4) Using the epithelial cells obtained in the above step (1), use the cell sheet technology (cellsheet) to c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a tissue engineering tracheal patch culture method and culture system. The method includes: (1) taking autogenous cartilage and epithelial cells for in-vitro culture; (2) performing 3D printing of a dish shaped bottom hard material layer with a flow passage system; (3) performing 3D printing of hydrogel cartilage cells above the bottom hard material layer to form a middle cell layer; (4) adopting a cell patch technique to construct a top patch layer, and transferring to the middle cell layer; (5) providing cartilage cell culture liquid and epithelial cell culture liquidto carry out hierarchical culture; (6) performing in-vitro culture to obtain a tissue engineering tracheal patch. By the tissue engineering tracheal patch cultured according to the method and system,problems of low tightness in combination of autogenous cells and artificial biological scaffolds and nonuniformity of allogeneic trachea donors and receptors are solved, the problem of cellular nutrition supply deficiency after patch transplanting is solved, and recovery of physiological functions of tracheas at repaired parts is facilitated.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a trachea patch, in particular to a method for culturing a tissue engineered trachea patch constructed by using a 3D printing composite tissue cell layered culture system and a culture system thereof. Background technique [0002] In the clinical work of thoracic surgery, patients with severe tracheal stenosis due to tumor, trauma and congenital stenosis are often encountered, resulting in dyspnea. Circular resection and reconstruction of the trachea is currently the most effective treatment option, but when the resection range of the trachea is too large (more than 50% of the total length in adults, and more than 30% of the total length in children), the resection margin cannot be "end-to-end" due to tension . At this time, in order to restore tracheal patency and respiratory function, it is necessary to implant a tracheal substitute with good biocompatibility. [0003] At pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/071C12M3/00
CPCC12M21/08C12M23/16C12M25/14C12N5/0697C12N2513/00C12N2533/54C12N2533/74C12N2533/80
Inventor 范子文张磊顾亚伟
Owner SHANGHAI PULMONARY HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap