Compound fungicide for straw fermentation and preparation method thereof
A compound bacterial agent and fermentation culture technology, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems such as difficult application of lignin, and achieve the effect of ensuring the number and proportion of viable bacteria and improving efficiency
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Embodiment 1
[0050] A compound bacterial agent capable of fermenting straw into fertilizer, the compound bacterial agent is prepared by the following preparation method:
[0051] Cellulovibrio, Rhizobium and Agrobacterium were mixed and cultured in bacterial seed medium at 35°C for 16 hours; then cultured in bacterial shaker medium at 35°C with a shaker speed of 160r / min for 16 hours; finally in Bacterial fermentation medium, 39 ℃, shaker speed 140r / min, fermentation culture for 24 hours, after the fermentation culture is completed, the mother liquid is obtained, which is the bacterial liquid;
[0052] The bacterial seed culture medium is made of the following components in percentage by weight: glucose 5%, peptone 5%, disodium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.5%, agar 1.5%, the balance is water, and the pH value is 7.5;
[0053] The bacterial shaker culture medium is made of the following components in percentage by weight: 3% glucose, 5% soybean meal, 0.5% beef ...
Embodiment 2
[0065] A compound bacterial agent capable of fermenting straw into fertilizer, the compound bacterial agent is prepared by the following preparation method:
[0066] Mix Vibrio cellulosus, Rhizobium and Agrobacterium, and culture them in bacterial seed medium at 39°C for 12 hours; then culture them in bacterial shaker medium at 39°C with a shaker speed of 140r / min for 20 hours; finally in Bacterial fermentation medium, 35°C, 160r / min shaker speed for 16 hours of fermentation, after the fermentation is completed, the mother liquor is obtained, which is the bacterial broth;
[0067] The bacterial seed culture medium is made of the following components in percentage by weight: glucose 10%, peptone 2%, disodium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, agar 2.5%, the balance is water, and the pH value is 7.0;
[0068] The bacterial shaker culture medium is made of the following components by weight percentage: 8% glucose, 3% soybean meal powder, 1% beef extrac...
Embodiment 3
[0080] A compound bacterial agent capable of fermenting straw into fertilizer, the compound bacterial agent is prepared by the following preparation method:
[0081] Mix Vibrio cellulosus, Rhizobium and Agrobacterium, and culture them in bacterial seed medium at 37°C for 14 hours; Bacterial fermentation medium, 37 ℃, shaker speed of 150r / min, fermentation culture for 20 hours, after the fermentation culture is completed, the mother liquid is obtained, which is the bacterial liquid;
[0082] The bacterial seed culture medium is made of the following components by weight percentage: glucose 7%, peptone 3%, disodium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.3%, agar 2%, the balance is water, and the pH is 7.2;
[0083] The bacterial shaker culture medium is made of the following components by weight percentage: 5% glucose, 4% soybean meal powder, 0.75% beef extract, 3.5% peptone, 0.2% magnesium sulfate, 0.25% disodium hydrogen phosphate, 0.25% dihydrogen phosphat...
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