Compound fungicide for straw fermentation and preparation method thereof

A compound bacterial agent and fermentation culture technology, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems such as difficult application of lignin, and achieve the effect of ensuring the number and proportion of viable bacteria and improving efficiency

Inactive Publication Date: 2018-08-28
北京航天恒丰科技股份有限公司
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aiming at the problem that lignin in straw is difficult to apply, the present invention uses a variety of different types of microorganisms (bacteria, actinomycetes and fungi) for compounding to obtain a relatively stable and reliable fermentation agent between microorganisms that can quickly decompose fermented straw, and ferment straw to biofertilizer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A compound bacterial agent capable of fermenting straw into fertilizer, the compound bacterial agent is prepared by the following preparation method:

[0051] Cellulovibrio, Rhizobium and Agrobacterium were mixed and cultured in bacterial seed medium at 35°C for 16 hours; then cultured in bacterial shaker medium at 35°C with a shaker speed of 160r / min for 16 hours; finally in Bacterial fermentation medium, 39 ℃, shaker speed 140r / min, fermentation culture for 24 hours, after the fermentation culture is completed, the mother liquid is obtained, which is the bacterial liquid;

[0052] The bacterial seed culture medium is made of the following components in percentage by weight: glucose 5%, peptone 5%, disodium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.5%, agar 1.5%, the balance is water, and the pH value is 7.5;

[0053] The bacterial shaker culture medium is made of the following components in percentage by weight: 3% glucose, 5% soybean meal, 0.5% beef ...

Embodiment 2

[0065] A compound bacterial agent capable of fermenting straw into fertilizer, the compound bacterial agent is prepared by the following preparation method:

[0066] Mix Vibrio cellulosus, Rhizobium and Agrobacterium, and culture them in bacterial seed medium at 39°C for 12 hours; then culture them in bacterial shaker medium at 39°C with a shaker speed of 140r / min for 20 hours; finally in Bacterial fermentation medium, 35°C, 160r / min shaker speed for 16 hours of fermentation, after the fermentation is completed, the mother liquor is obtained, which is the bacterial broth;

[0067] The bacterial seed culture medium is made of the following components in percentage by weight: glucose 10%, peptone 2%, disodium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, agar 2.5%, the balance is water, and the pH value is 7.0;

[0068] The bacterial shaker culture medium is made of the following components by weight percentage: 8% glucose, 3% soybean meal powder, 1% beef extrac...

Embodiment 3

[0080] A compound bacterial agent capable of fermenting straw into fertilizer, the compound bacterial agent is prepared by the following preparation method:

[0081] Mix Vibrio cellulosus, Rhizobium and Agrobacterium, and culture them in bacterial seed medium at 37°C for 14 hours; Bacterial fermentation medium, 37 ℃, shaker speed of 150r / min, fermentation culture for 20 hours, after the fermentation culture is completed, the mother liquid is obtained, which is the bacterial liquid;

[0082] The bacterial seed culture medium is made of the following components by weight percentage: glucose 7%, peptone 3%, disodium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.3%, agar 2%, the balance is water, and the pH is 7.2;

[0083] The bacterial shaker culture medium is made of the following components by weight percentage: 5% glucose, 4% soybean meal powder, 0.75% beef extract, 3.5% peptone, 0.2% magnesium sulfate, 0.25% disodium hydrogen phosphate, 0.25% dihydrogen phosphat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a compound fungicide capable of fermenting straws into fertilizer and a preparation method thereof. The method uses various different categories of microorganisms (bacteria, actinomycetes and fungus) for compounding by aiming at a problem that lignin in the straw is difficult to be applied. The obtained microorganisms are stable and reliable, fermentation fungicides for straw fermentation can be quickly decomposed, and straws are fermented to obtain biofertilizer. The straws are processed, environment is protected, the biofertilizer with high humic acid and organic matter content and conforms to harmless index requirements is obtained, and wastes are made profitable so as to kill two birds with one stone. The preparation method is characterized in that different categories of microorganisms are cultured by different culture media and fermentation methods and are mixed, the ratio of various microorganisms can be guaranteed to be balanced while the number of viablebacteria is guaranteed, and a situation of small quantity since a certain microorganism has weak ability of struggling for existence can be avoided.

Description

technical field [0001] The invention belongs to the technical field of fermentation strains, and in particular relates to a composite bacterial agent capable of fermenting straw into fertilizer and a preparation method thereof. Background technique [0002] As we all know, in the vast rural areas of our country, a large amount of agricultural fertilizers are industrially produced inorganic fertilizers. Although they have a significant effect on increasing agricultural production, due to long-term single or partial application of chemical fertilizers, the growth and reproduction of microbial groups and biological enzyme groups in the soil are inhibited. And positive effect, lead to serious reduction of organic matter in soil, serious compaction of soil, decline of comprehensive fertility, destruction of ecological environment, malnutrition of crops, weakening of autoantibodies, poor product quality, accumulation of toxins in food, endangering human and animal health. [0003]...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F11/08C12R1/63C12R1/41C12R1/01C12R1/885
CPCC05F11/08C12N1/14C12N1/20
Inventor 刘海明
Owner 北京航天恒丰科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap