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Enteromorpha-sourced angiotensin invertase inhibitory peptide and preparation method and application thereof

An angiotensin and inhibitory peptide technology, applied in the field of marine functional peptides and their preparation, can solve the problems of no further application, inability to determine the primary results of peptides, and few ACE inhibitory peptides, etc., and achieves important market value and application prospects. Toxic side effects, the effect of strong antihypertensive activity

Active Publication Date: 2018-09-04
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ningbo University from the marine microalga Isoflagellates ( Isochrysis ) isolated and purified ACE antihypertensive peptide, its structure is Tyr--Met-Gly-Leu-Asp-Leu-Lys (patent name: an angiotensin-converting enzyme inhibitory peptide derived from marine microalgae, ZL201410172674.X ), but there are still relatively few ACE inhibitory peptides from seaweed, many of which cannot be further applied because the primary results of the peptides cannot be determined

Method used

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  • Enteromorpha-sourced angiotensin invertase inhibitory peptide and preparation method and application thereof
  • Enteromorpha-sourced angiotensin invertase inhibitory peptide and preparation method and application thereof
  • Enteromorpha-sourced angiotensin invertase inhibitory peptide and preparation method and application thereof

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Embodiment Construction

[0024] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

1. Specific examples

[0025] Angiotensin-converting enzyme inhibitory peptide derived from Enteromorpha enteromorpha, the amino acid sequence of the inhibitory peptide is SEQ ID NO1: Phe-Gly-Met-Pro-Leu-Asp-Arg (FGMPLDR) and / or SEQ ID NO 2: Met-Glu -Leu-Val-Leu-Arg (MELVLR). Its preparation method comprises the following steps:

[0026] (1) Preparation of Enteromorpha protein: add fresh Enteromorpha to 2.0wt% sodium chloride solution at a mass ratio of 1:3, mash it, repeatedly freeze-thaw it, extract it with ultrasonic waves, leaching overnight, 8000r / min after centrifugation for 15 minutes; take the supernatant and add ammonium sulfate to precipitate and concentrate. After centrifugation, take the precipitate and redissolve it in distilled water. After passing through a 0.45 μm filter membrane, use a dialysis bag with a molecular weight cu...

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Abstract

The invention discloses an enteromorpha-sourced angiotensin invertase inhibition peptide and a preparation method and an application thereof. An amino acid sequence of the inhibition peptide is SEQ IDNO1: Phe Gly Met Pro Leu Asp Argand / or SEQ ID NO 2: Met Glu Leu Val Leu Arg, the preparation method comprises a step of preparing enteromorpha crude protein; adding trypsin in an enteromorpha crude protein solution for enzymatic hydrolysis to obtain an enzymatic hydrolysis product; performing ultrafiltration on the enzymatic hydrolysis product, separating the material, and performing gel chromatography; collecting a third absorption peak component containing an ACE inhibition peptide; performing gel chromatography on the component containing the ACE inhibition peptide, and employing a high performance liquid chromatography for separating, and collecting the eighth absorption peak component containing the ACE inhibition peptide to obtain the inhibition peptide. The enteromorpha-sourced angiotensin invertase inhibition peptide has the advantages of strong antihypertensive activity, high security, no toxic and side effect, and easy absorption by human body.

Description

technical field [0001] The present invention relates to an angiotensin-converting enzyme inhibitory peptide and a preparation method thereof, in particular to a marine functional polypeptide with angiotensin-converting enzyme (ACE) inhibitory activity isolated and purified from the large marine green alga Enteromorpha and its preparation methods and applications. Background technique [0002] Angiotensin converting enzyme (ACE) is a zinc metalloprotease and one of the important proteases in the renin-angiotensin system. It plays an important role in the regulation of human blood pressure. By acting on the end of angiotensin I to remove His-Leu to generate angiotensin II, it can contract arterial smooth muscle and rapidly cause blood pressure to rise. Inhibiting ACE activity is an effective way to lower blood pressure. Most of the current drugs for the treatment of hypertension are chemical synthetic products, which have some adverse reactions, such as cough, taste disturba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/34C07K1/16C07K1/36C07K1/14
CPCC07K7/06C12P21/06
Inventor 徐年军孙雪徐晓婷操德群李亚鹤胡朝阳
Owner NINGBO UNIV
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