Enteromorpha-sourced angiotensin invertase inhibitory peptide and preparation method and application thereof
An angiotensin and inhibitory peptide technology, applied in the field of marine functional peptides and their preparation, can solve the problems of no further application, inability to determine the primary results of peptides, and few ACE inhibitory peptides, etc., and achieves important market value and application prospects. Toxic side effects, the effect of strong antihypertensive activity
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[0024] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
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[0025] Angiotensin-converting enzyme inhibitory peptide derived from Enteromorpha enteromorpha, the amino acid sequence of the inhibitory peptide is SEQ ID NO1: Phe-Gly-Met-Pro-Leu-Asp-Arg (FGMPLDR) and / or SEQ ID NO 2: Met-Glu -Leu-Val-Leu-Arg (MELVLR). Its preparation method comprises the following steps:
[0026] (1) Preparation of Enteromorpha protein: add fresh Enteromorpha to 2.0wt% sodium chloride solution at a mass ratio of 1:3, mash it, repeatedly freeze-thaw it, extract it with ultrasonic waves, leaching overnight, 8000r / min after centrifugation for 15 minutes; take the supernatant and add ammonium sulfate to precipitate and concentrate. After centrifugation, take the precipitate and redissolve it in distilled water. After passing through a 0.45 μm filter membrane, use a dialysis bag with a molecular weight cu...
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