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A kind of medical dressing of stem cell extract and preparation method thereof

A stem cell and extract technology, applied in the field of biomedicine, can solve the problems of breeding bacteria, hindering the rapid healing of wounds, slow absorption, etc., to achieve the effect of promoting skin tissue, excellent synergy, and weak immunogenicity

Active Publication Date: 2020-11-06
大美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, previous studies mainly focused on wound treatment by collecting culture medium supernatant, which contained less effective active cytokines and many foreign substances, including apoptotic cells and cell debris. Difficult to store stably, easy to pollute, causing many negative problems, not conducive to standardized production and preparation
Existing technologies cannot maximize the use of active ingredients in stem cell cultures. Existing technologies extract cytokines secreted by stem cells, mostly using dialysis, and the resulting extracts have very limited effects on wound healing
[0011] 2. Most of the gel dressings in the prior art are cell suspensions made of stem cells, and the activity is difficult to maintain. In the suspension state, the activity of stem cells decreases rapidly (the best use time is within 48 hours), and its application has great limitations. nature; and almost all wound injuries are sudden, accidental and unpredictable, which requires the application of dressings in the shortest time for treatment, and wait until the cells are cultured before making cell suspensions, which has missed the best time. Timing of treatment
[0012] 3. Some technologies of prior art dressings do not use antibacterial agents, which are easy to breed bacteria during use and cause secondary pollution. The antibacterial agents used in another part of the technology include silver ions, quaternary ammonium salts, etc. Silver ions have certain side effects on the human body, and When the skin is very sensitive, quaternary ammonium salts are likely to cause irritation to the wound, affect the patient's experience of use, and hinder the rapid healing of the wound; and there will be certain side effects after being absorbed by human tissue
[0013] 4. The gels used in the prior art dressings mainly include gelatin and bioactive glass, which are slow to absorb and have insufficient affinity to the skin
[0014] 5. The dressings in the prior art cannot effectively prevent the skin from leaving scars, and skin care products are still needed to dilute the scars after the wound is repaired
[0015] 6. The dressings of the prior art are unsatisfactory for chronic wound repair, and cannot effectively control exudate and repair damaged tissues, blood vessels and nerves

Method used

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  • A kind of medical dressing of stem cell extract and preparation method thereof
  • A kind of medical dressing of stem cell extract and preparation method thereof
  • A kind of medical dressing of stem cell extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, preparation stem cell extract

[0065] (1) Preparation of BMSCs extract

[0066] Take the purchased embryo-derived BMSCs cell P4 out of the liquid nitrogen refrigerator, quickly place it in a 37°C water bath and shake it to melt, centrifuge the cryovial at 1500rpm for 5min, discard the supernatant, and resuspend with 1ml recovery medium ;

[0067] The recovery medium is DMEM-F12 medium containing 15% fetal bovine serum, and the cell count is 8*10 5 Cells were seeded on 75cm 2 In the culture bottle, add the medium in the culture bottle to 15ml;

[0068] Place the flask at 37°C, 5% CO 2 Cultivate in an incubator, change the medium after 24 hours (DMEM-F12 medium containing 10% fetal bovine serum), and then change the medium every 2 days. After 3-5 days, the cell fusion reaches more than 90% for passage;

[0069] When passaging, first discard the medium, wash twice with 5-10ml PBS, add 2ml 0.05% trypsin for digestion, shake gently to make the trypsin cov...

Embodiment 2

[0088] Embodiment 2, preparation collagen freeze-dried powder

[0089] (1) Extraction of fish skin collagen;

[0090] Step 1, tissue crushing: After thawing the skin of adult salamander (artificial breeding) weighing 2-3 catties, add water so that the weight ratio of fish skin to water is 10:1, crush and grind the mixture, and then crush Ultrasonic action is added while grinding, the ultrasonic intensity is controlled at 750 watts / liter, and the ultrasonic treatment time is 40min to obtain a slurry;

[0091] Step 2, remove salt-soluble impurities: mix the slurry with sodium chloride solution (mass concentration 5%), the weight ratio of slurry and sodium chloride solution is 1:5, stir well, soak for 15h, 3000rpm, centrifuge for 20min, Remove the supernatant. get the first precipitation;

[0092] Step 3, remove acid-soluble impurities: mix the first precipitate with citric acid solution (mass concentration is 10%), the weight ratio of the first precipitate and acid solution is...

Embodiment 3

[0102] Embodiment 3, preparation medical dressing

[0103] 100g of medical dressings contains: bone marrow mesenchymal stem cell (BMSCs) extract lyophilized powder prepared in Example 1 (mass fraction 0.2%), collagen lyophilized powder prepared in Example 2 (mass fraction 0.1%); sodium alginate (mass fraction 2%); concentrated vitamin C powder (10%); normal saline (mass fraction 87.7%).

[0104] When in use, just mix the powder and water evenly. 10g of the dressing contains 20mg of BMSCs extract freeze-dried powder, 10mg of collagen freeze-dried powder, 0.2g of sodium alginate, 1g of concentrated vitamin C powder, and 8.77g of normal saline.

[0105] 10g dressing can be applied no larger than 10 x 10cm 2 wound surface.

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Abstract

The invention discloses a stem cell extract product medical dressing, and a preparation method thereof, and belongs to the technical field of biological medicine. The stem cell extract product medicaldressing is a wound cover material with excellent effect, is capable of preventing bacterium infection effectively, accelerating wound healing, controlling and absorbing wound exudate, and protectingnew born tissues, possesses excellent biocompatibility, and is excellent in permeability; no toxic or side effect is caused; natural green raw materials are adopted; the adopted accessory materials are edible grade; too much bleeding and patient pain are avoided; delaying of wound healing caused by left residues is avoided; and an environment beneficial for wound healing is providing for promotion of skin regeneration. The stem cell extract product medical dressing is capable of controlling wound exudates, supplying water and maintaining humidity, relieving inflammation and swelling, relieving wound pain and other discomforts, avoiding scars left by wound, and avoiding influences on skin appearance. The stem cell extract product medical dressing is prepared through freeze drying, so thatit is more beneficial for maintenance of the activity of a large amount of cytokines and proteins, and storage and transportation under room temperature conditions can be realized.

Description

technical field [0001] The invention relates to a medical dressing of stem cell extract and a preparation method thereof, belonging to the field of biomedicine. Background technique [0002] The skin is the largest organ of the human body and acts as a barrier to protect the body. However, it is the most vulnerable to trauma. Common traumas caused by sharp objects and tools are acute wounds, which are prone to blood loss and infection, and invade the dermis. Painful and easy to leave scars, which affect the appearance of the skin; other common ones include abrasions, burns, scalds, and frostbite. This kind of trauma is a chronic wound, which generally causes soft tissue contusion, swelling and pain, and often has wound exudate. Causes blood vessel rupture and nerve damage in skin tissue and muscle, which may be more difficult to heal than acute wounds. Therefore, when the skin is wounded, it should be taken care of in time and effectively to prevent unnecessary sequelae. In...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A61L15/40A61L15/32A61L15/44A61L15/46A61L15/20A61L15/28
CPCA61L15/20A61L15/28A61L15/325A61L15/40A61L15/44A61L15/46A61L2300/216A61L2300/404A61L2300/41A61L2300/412A61L2300/426C12N5/0663C12N2500/34C12N2500/38C12N2500/90C08L5/04
Inventor 周萱
Owner 大美生物科技有限公司
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