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Rice seed priming method

A rice seed and seed technology, applied in botany equipment and methods, biochemical equipment and methods, fermentation, etc., can solve problems such as difficulty in determining the best time, and achieve the effect of improving the priming effect of seeds

Active Publication Date: 2018-09-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the determination of the best time for priming mainly uses the method of seed germination test, and it is difficult to determine the best time for priming due to different environments and plant varieties.

Method used

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  • Rice seed priming method
  • Rice seed priming method
  • Rice seed priming method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Comparison of effects of different initiation times of rice

[0023] Using the rice variety Wuyunjing No. 7, 50 healthy and plump seeds were repeatedly selected each time, sterilized with 0.1% mercuric chloride solution for 5 minutes, rinsed with distilled water for 3 times, wiped the surface of the seeds and placed them in a bag with two layers of filter paper. Add 10mL of distilled water to a petri dish (diameter 9cm), place it in a dark incubator at 30°C to absorb water for 0, 4, 8, 12, 18, 24, 30, and 36 hours, then dry the seeds slowly at 25°C. to the initial moisture content of the seeds (about 13%). The experiment was repeated 3 times.

[0024] With the untriggered seeds as the control, the germination test was carried out on the primed seeds. Germination method: Repeatedly select 50 healthy and plump seeds each time, disinfect the surface with 0.1% mercuric chloride solution for 5 minutes, rinse with distilled water for 3 times, and wipe the surfa...

Embodiment 2

[0026] Example 2: Analysis of gene expression during rice seed priming

[0027] Using the rice variety Wuyunjing No. 7, 50 healthy and plump seeds were repeatedly selected each time, sterilized with 0.1% mercuric chloride solution for 5 minutes, rinsed with distilled water for 3 times, wiped the surface of the seeds and placed them in a bag with two layers of filter paper. Add 10 mL of distilled water to a Petri dish (diameter 9 cm), place it in a dark incubator at 30°C to absorb water for 0, 4, 8, 12, 18, 24, 30, and 36 hours, and then take samples. The seeds were frozen in liquid nitrogen and quickly ground into powder, and the samples were stored at -80°C. The experiment was repeated 3 times.

[0028] Use the TransZol Plant kit (Transgen, www.transgen.com) kit to extract the RNA of each sample; II Reverse Transcriptase system (Vazyme Biotech Co., Ltd) kit reverse-transcribes to form cDNA, which is used as a template; it is analyzed by fluorescent quantitative PCR, and fl...

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Abstract

The invention discloses a rice seed priming method. An optimal priming time can be confirmed by detecting OsIPMS1 gene expression in the priming process of seeds. The gene, namely isopropylmolic acidOsIPMS1, has a nucleotide sequence SEQ ID NO.1 as shown in the specification; gene expression of the seeds in the water priming process can be detected in real time through fluorescent quantitative PCR (Polymerase Chain Reaction), and the gene has an upstream primer sequence of SEQ ID NO.2 as shown in the specification, and a downstream primer sequence of SEQ ID NO.3 as shown in the specification.Tests show that in the water priming process of seeds at 30 DEG C, before gene expression is remarkably degraded, that is, 8 hours after priming treatment, swelling is terminated, re-drying treatmentis carried out at 25 DEG C, and primed seeds have optimal seed activity. Tests show that the gene can be used as a molecular marker for monitoring the rice seed priming process, and confirming optimal seed priming times.

Description

technical field [0001] The invention relates to the technical field of seed treatment, in particular to a priming method for rice seeds. Background technique [0002] Seed initiation is to control the water absorption of seeds in the second stage, so that the pre-germinated macromolecules, cell membranes and organelles of the seeds are repaired and activated. stick out. Seed priming can promote seed germination, improve the uniformity of seed emergence, shorten the emergence time, and improve seedling quality and stress resistance. As a simple, effective and easy-to-operate method, seed priming has been paid more and more attention to agricultural production. [0003] Water priming is the most simple and low-cost seed priming method. In the present invention, the seeds are placed in water for imbibition for a period of time, and then the seeds are dried back at room temperature. Water priming is mainly to control the water supply conditions and water absorption time to en...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12Q1/6895
CPCC12N9/1025C12Q1/6895C12Q2600/158C12Q2600/166C12Y203/03013
Inventor 王州飞何永奇
Owner SOUTH CHINA AGRI UNIV
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