Composition used for improving immune function and application thereof

A technology of immune function and composition, applied in the field of medicine, can solve problems such as safety and side effects

Active Publication Date: 2018-09-07
ZHEJIANG HISUN PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Now some immunopotentiating agents (immunopotentiating agents) such as levamisole are used to alleviate leukopenia in chemotherapy patients, but these drugs also have significant safety and side effects

Method used

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  • Composition used for improving immune function and application thereof
  • Composition used for improving immune function and application thereof
  • Composition used for improving immune function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: sample preparation

[0040] (1) Bacteria powder sample preparation: Activate the -80°C frozen glycerol tube strains of Lactobacillus plantarum HPL03, Lactobacillus rhamnosus HCL01, and Lactobacillus reuteri HCL04 on Rogosa solid medium, and culture them anaerobically at 37°C for 40 hours Pick a single colony and transfer it to a test tube containing 5ml of liquid medium (MRS Broth), and culture it anaerobically at 37°C for 24h; In the Erlenmeyer shake flask, anaerobic static culture at 37°C for about 18h; collect the fermentation broth, centrifuge at 4000rpm at 4°C for 10min, discard the supernatant, and wash twice with PBS (Cat. Finally, 20ml of PBS buffer was used to suspend the bacteria, and the concentrations of the three bacteria were controlled at 10 9 ~10 12 CFU / ml, mix, add equal volume of lyoprotectant liquid solution (8% skimmed milk powder, 3% lactose, 1% maltodextrin, 0.5% sodium glutamate, 0.5% ascorbic acid, the rest is water, and each subs...

Embodiment 2

[0043] Embodiment 2: animal experiments

[0044] (1) Animal grouping: BALB / c mice, SPF grade, provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., license number: SCXK (Beijing) 2012-0001; 40 BALB / c mice, 7 weeks Age, 20-22g, male, were randomly divided into 5 groups, 8 rats in each group, respectively control group, model group, test group 1, test group 2 and test group 3.

[0045] (2) Administration: The mice were administered by intragastric administration. The specific control group and model group administered PBS solution to the mice, and the test group administered the following samples to the mice: Test group 1: 4mg SA / Mice / day, test group 2: 4mg HA / mouse / day, test group 3: 4mg SHA / mouse / day. Specifically, 40 mg of samples were taken out from 4°C every day, adjusted to 5 ml with PBS buffer, mixed thoroughly, and 0.5 ml was administered to each mouse once a day for 21 consecutive days.

[0046] (3) Modeling: the animal model used in this test i...

Embodiment 3

[0047] Example 3: Detection of conventional immune indicators

[0048] (1) Detection method:

[0049] On the 5th day after modeling, use a blood cell analyzer to detect the number of red blood cells, platelets, white blood cells and lymphocytes in the tail vein blood of the mice; and take thymus and spleen, weigh them, and calculate the immune organ index.

[0050] Immune organ index = immune organ weight / body weight

[0051] (2) Statistical methods

[0052] The data obtained from the experiment are expressed as (MEAN±SD), and the differences among groups were compared by t test.

[0053] (3) Analysis of results:

[0054] A. The results of mouse organ analysis of each experimental group are shown in Table 1.

[0055] Table 1: Effects of different administrations on the immune organs of immunocompromised mice

[0056]

[0057] Note: a: The average molecular weight of HA is about 180,000-220,000 Daltons; b: the hyaluronic acid in the SHA sample is HA, and the average mol...

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Abstract

The invention belongs to the field of medicines, and provides a composition used for improving the immune function and a preparation method and application thereof. The active ingredients of the composition are hyaluronic acid or hyalurate and lactobacilli. The composition can improve the organism immune function, the composition is particularly suitable for relieving and treating complications induced by the anti-tumor chemotherapy, and the side effects of chemotherapeutic drugs are reduced.

Description

technical field [0001] The invention belongs to the field of medicine and relates to a composition for improving immune function. Background technique [0002] So far, most of the anticancer drugs used in chemotherapy or radiotherapy have side effects such as cytotoxicity to patients in varying degrees, resulting in decreased immunity of patients, which in turn increases the risk of intestinal infection and multiple organ failure, and also seriously affects cancer. Patient's quality of life. People gradually realize that in the process of cancer treatment, it is necessary not only to pursue the curative effect, but also to find all ways to reduce the side effects caused by drugs. [0003] Cyclophosphamide (CY), as a broad-spectrum anticancer drug in clinical practice, mainly kills lymphocytes non-specifically by destroying DNA synthesis through DNA alkylation, and can inhibit lymphocyte transformation. Its curative effect is significant, but It is often accompanied by very...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/744A61K35/747A61K35/745A61P37/04A23L33/135A23L33/125A61K31/728
CPCA61P37/04A23L33/125A23L33/135A61K31/728A61K35/744A61K35/745A61K35/747A23V2002/00A23V2400/173A23V2400/175A23V2400/169A61K2300/00A23V2200/324A23V2200/32A23V2250/5114A23V2250/612A23V2250/708A23V2250/62A23V2300/10
Inventor 党福军林海鸣周军王欣郑晓鹤徐晴雨李娜黄隽
Owner ZHEJIANG HISUN PHARMA CO LTD
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