Biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil and preparation and application thereof

A technology of biological bacterial fertilizer and soil improvement, applied in the direction of chemicals, applications, biocides, etc. for biological control, can solve the problem that the root rot of flue-cured tobacco cannot be fundamentally eliminated, and the pursuit of farmers' economic interests is difficult to achieve. Polluted water bodies and pesticide bioaccumulation and other problems, to achieve the effect of improving root rot resistance, inducing plant resistance resistance, good antagonistic and competitive effects

Inactive Publication Date: 2018-09-21
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although crop rotation can reduce the occurrence of diseases, it is difficult to achieve due to the lack of high-quality tobacco planting land in my country and the pursuit of economic benefits by farmers.
Cultivation measures such as high-ridge transplanting, shallow irrigation and water control can control the occurrence of flue-cured tobacco root rot to a certain extent, but they cannot fundamentally eliminate the occurrence of flue-cured tobacco root rot; the use of disease-resistant varieties can minimize the occurrence of disease and popular, but as a special economic crop, the internal quality of flue-cured tobacco is more important than disease resistance, and it is difficult to find new varieties of flue-cured tobacco that can not only be highly resistant to root rot but also meet the internal quality requirements; chemical fungicides are fast and effective. It has become the main measure to prevent and control flue-cured tobacco root rot, but the large-scale use of chemical pesticides will pollute water bodies and bioaccumulate pesticides, causing great harm to human health and the ecological environment
At the same time, the long-term use of chemical fungicides has made many pathogens such as Pythium, Fusarium, and Phytophthora resistant to them, and even mutated, resulting in a decline in the control effect, and even no effect on root rot.

Method used

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  • Biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil and preparation and application thereof
  • Biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil and preparation and application thereof
  • Biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil and preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] A biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil, each component in the biological bacterial fertilizer is made according to the following ratio:

[0047] Biological fertilizer seed bacteria fermentation broth (volume percentage: Trichoderma harzianum 20%, Bacillus megaterium 15%, Bacillus subtilis 20%, Bacillus pumilus 15%, Trichoderma viride 15%, Lactobacillus acidophilus 15%), peanut bran;

[0048] The preparation method of the biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil comprises the following steps:

[0049] A. Insert Trichoderma harzianum in a dormant state stored in sand tubes and freeze-drying tubes into Chapei solid medium, and Bacillus megaterium, Bacillus subtilis and Bacillus pumilus into beef extract peptone solid medium , Trichoderma viride and lactic acid bacteria were inserted into the MRS solid medium, and each strain used a separate medium to avoid contami...

Embodiment 2

[0060] A biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil, each component in the biological bacterial fertilizer is made according to the following ratio:

[0061] Biological fertilizer seed bacteria fermentation broth (volume percentage: Trichoderma harzianum 15%, Bacillus megaterium 20%, Bacillus subtilis 20%, Bacillus pumilus 20%, Trichoderma viride 15%, Lactobacillus acidophilus 10%), peanut bran;

[0062] The preparation method of the biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil comprises the following steps:

[0063] A. Insert Trichoderma harzianum in a dormant state stored in sand tubes and freeze-drying tubes into Chapei solid medium, and Bacillus megaterium, Bacillus subtilis and Bacillus pumilus into beef extract peptone solid medium , Trichoderma viride and lactic acid bacteria were inserted into the MRS solid medium, and each strain used a separate medium to avoid contami...

Embodiment 3

[0074] A biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil, each component in the biological bacterial fertilizer is made according to the following ratio:

[0075] Biological fertilizer seed bacteria fermentation liquid (volume percentage: Trichoderma harzianum 10%, Bacillus megaterium 20%, Bacillus subtilis 20%, Bacillus pumilus 20%, Trichoderma viride 15%, Lactobacillus acidophilus 15%), peanut bran;

[0076] The preparation method of the biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil comprises the following steps:

[0077] A. Insert Trichoderma harzianum in a dormant state stored in sand tubes and freeze-drying tubes into Chapei solid medium, and Bacillus megaterium, Bacillus subtilis and Bacillus pumilus into beef extract peptone solid medium , Trichoderma viride and lactic acid bacteria were inserted into the MRS solid medium, and each strain used a separate medium to avoid contamin...

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Abstract

The invention discloses a biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil and preparation and application thereof. The biological bacterial fertilizer isprepared from biological bacterial fertilizer seed bacteria fermentation broth (5-20% of trichoderma harzianum, 5-20% of bacillus megatherium, 5-20% of bacillus subtilis, 5-20% of bacillus pumilus, 5-20% of trichoderma viride and 5-20% of lactobacillus acidophilus) and peanut bran; and the mass ratio of the biological bacterial fertilizer seed bacteria fermentation broth to the peanut bran is 1:3-8. The trichoderma harzianum, bacillus megatherium, bacillus subtilis, bacillus pumilus and trichoderma viride in the biological bacterial fertilizer have very good antagonism and competition effectson root rot pathogen, can effectively inhibit life activities of the root rot pathogen, induce plant resistance to resist, and improve the ability of the flue-cured tobacco to resist the root rot; thelactobacillus acidophilus can effectively improve soil and reduce the growth of weeds; the biological bacterial fertilizer is safe to the environment, pollution-free, has no residue, strong specificity, high activity and no side effect on non-target bio-safety.

Description

technical field [0001] The invention relates to a preparation method of biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil, in particular to a biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil, a preparation method and application thereof. Background technique [0002] Root rot is one of the common diseases in the field growth of flue-cured tobacco. It is caused by Pythium, Fusarium, Phytophthora and other pathogens. It is a fungal soil-borne disease. Due to the low rotation rate of tobacco fields in my country, flue-cured tobacco The phenomenon of continuous planting is serious. In recent years, the incidence of root rot in the main flue-cured tobacco production areas in my country has become higher and higher. In tobacco fields where the disease occurs year after year in dry land, the incidence rate of root rot can reach more than 50%, resulting in the death or even failure of tobacco pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/20A01N63/04A01N63/02A01P3/00C09K17/14A01B79/02A01C21/00A01G22/45C09K101/00
CPCA01B79/02A01C21/00A01G22/45A01N63/10A01N63/30A01N65/00A01N65/20C09K17/14C09K2101/00
Inventor 王维宗钊辉吴有祥黄浩谢晋许洪庆贾海江
Owner SOUTH CHINA AGRI UNIV
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