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Preparation and application for growth-promoting trichoderma and bacillus composite wettable powder

A technology of wettable powder and bacillus, applied in the direction of application, biocide, fungicide, etc., can solve the problem of different types of antagonistic secondary metabolites, the effect of preventing and controlling bacterial diseases is not outstanding, and the effect of preventing and controlling bacillus soil-borne diseases is not as good Trichoderma and other problems, to achieve the effect of improving resistance to stress factors, improving fruit yield and quality, and improving nutrient utilization efficiency

Active Publication Date: 2018-09-28
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Previous studies have proved that Trichoderma and Bacillus are commonly used biocontrol microorganisms in the world, but the single use of these two microorganisms has certain limitations
On the one hand, the single use of Trichoderma is not effective in preventing and controlling bacterial diseases, while Bacillus is not as effective in controlling soil-borne diseases as Trichoderma; on the other hand, Trichoderma has significant functions of soil restoration, degradation or adsorption of agrochemical substances , while Bacillus has a strong growth-promoting effect, but the types of antagonistic secondary metabolites produced by the two microorganisms are significantly different
In addition, Trichoderma has obvious hyperparasitic and competitive effects on pathogenic fungi, and Bacillus also has outstanding antagonistic effects and stress resistance; therefore, there is no relevant research on the synergistic use of these two types of microorganisms

Method used

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  • Preparation and application for growth-promoting trichoderma and bacillus composite wettable powder
  • Preparation and application for growth-promoting trichoderma and bacillus composite wettable powder
  • Preparation and application for growth-promoting trichoderma and bacillus composite wettable powder

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Trichoderma aculeatus GDSF1009 is prepared into Trichoderma aculeatus parent powder through plate inoculation, activation culture, fermentation, centrifugation, and preparation drying. Specifically include:

[0048] (1) Plate activation and multiplication of Trichoderma aculeatus: inoculate Trichoderma aculeatus GDSF1009 on a PDA petri dish, and culture it upside down in an incubator at 28° C. for 3-4 days.

[0049] PDA medium: 200g of boiled and filtered supernatant juice of potatoes, 20g of glucose, 20g of agar powder, distilled to 1L of purified water, autoclaved at 121°C for 30min.

[0050] (2) Preparation of Trichoderma aculeatus seed fungi: 200 g of potatoes, 20 g of glucose, and 1 L of purified water were dispensed into 250 mL Erlenmeyer flasks, and autoclaved at 121° C. for 25 min. Wash the conidia from the PDA Trichoderma colony with sterile water and adjust to 1.5 x 10 6 The concentration of CFU / mL was inserted into the liquid medium, placed in a shaker f...

Embodiment 2

[0080] 1. Trichoderma aculeatus GDSF1009 is prepared into Trichoderma aculeatus parent powder through plate inoculation, activation culture, fermentation, centrifugation, and preparation drying.

[0081] 2. The Bacillus amyloliquefaciens ACCC11060 is plate-inoculated, activated, fermented and dried to prepare the Bacillus amyloliquefaciens parent powder.

[0082] 3. Mix the above-mentioned Trichoderma aculeatus mother powder and Bacillus amyloliquefaciens mother powder evenly according to the mass ratio of 1:8 to make a composite wettable powder mother powder, and then add 1% dihydrogen phosphate to the total mass of the mother powder Potassium + 1% crystalline magnesium sulfate + 1% sodium tetraborate decahydrate, or add 1% potassium humate + 1% crystalline magnesium sulfate, and mix thoroughly with a mixer to obtain a growth-promoting Trichoderma·Bacillus composite wettable powder.

[0083] 4. Indoor potted soil mixing test:

[0084] Disinfect a sufficient amount of cucumbe...

Embodiment 3

[0089] 1. Trichoderma aculeatus GDSF1009 is prepared into Trichoderma aculeatus parent powder through plate inoculation, activation culture, fermentation, centrifugation, and preparation drying.

[0090] 2. The Bacillus amyloliquefaciens ACCC11060 is plate-inoculated, activated, fermented and dried to prepare the Bacillus amyloliquefaciens parent powder.

[0091] 3. Mix the above-mentioned Trichoderma aculeatus mother powder and Bacillus amyloliquefaciens mother powder evenly according to the mass ratio of 1:8 to make a composite wettable powder mother powder, and then add 1% dihydrogen phosphate to the total mass of the mother powder Potassium + 1% crystalline magnesium sulfate + 1% sodium tetraborate decahydrate, or add 1% potassium humate + 1% crystalline magnesium sulfate, and mix thoroughly with a mixer to obtain a growth-promoting Trichoderma·Bacillus composite wettable powder.

[0092] 4. Indoor root irrigation test:

[0093] Test laboratory: Shanghai Jiaotong Universi...

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Abstract

The invention discloses preparation and application for growth-promoting trichoderma and bacillus composite wettable powder. The preparation adopts a trichoderma asperegillum GDSF1009 strain and bacillus amyloliquefaciens ACCC11060 as fermentation strains of a composite preparation, and the trichoderma asperegillum GDSF1009 strain and the bacillus amyloliquefaciens ACCC11060 are respectively usedto prepare mother powder of the trichoderma asperegillum and mother powder of the bacillus amyloliquefaciens; and salt auxiliary agents such as magnesium sulfate, borax, potassium dihydrogen phosphateand potassium humate are added, mixing is performed, and therefore the final product is prepared. The composite wettable powder disclosed by the invention can be used for soaking seeds, mixing soil,irrigating roots and performing foliar spraying, and can improve a germination rate and a seedling emergence rate of the seeds; the composite wettable powder can promote seedling growth, and increasea seedling fresh weight; the composite wettable powder can increase yield and quality of melons, fruits and vegetables; and the composite wettable powder can effectively prevent and control leaf diseases such as cucumber anthracnose and gray mold and other soil-borne diseases such as rice sheath blight and corn stalk rot.

Description

technical field [0001] The invention belongs to the field of microbial fertilizers, and in particular relates to a preparation method of a growth-promoting trichoderma and bacillus composite wettable powder and its application in aspects such as promoting the growth of crops and vegetables and preventing and controlling diseases. Background technique [0002] Trichoderma is a beneficial fungus commonly found in soil and rhizosphere ecosystems. After Trichoderma colonizes the roots of plants, it can improve the resistance of vegetables and crops to abiotic stress and the utilization and uptake of nutrients. At the same time, it can produce or secrete a variety of compounds. On the one hand, these substances can stimulate crop growth and increase yield. On the other hand, they can induce local or systemic disease resistance in plants, and have good control of various harmful fungi and microorganisms in the soil. effect. [0003] Bacillus amyloliquefaciens belongs to the genus...

Claims

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Application Information

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IPC IPC(8): A01N25/14A01N63/04A01N63/00A01P3/00A01P21/00C12N1/20C12N1/14C09K17/14C09K17/40C12R1/885C09K101/00
CPCA01N25/14A01N63/00A01N63/30C09K17/14C09K17/40C09K2101/00C12N1/14C12N1/20
Inventor 刘振阳刘佳陈捷
Owner SHANGHAI JIAO TONG UNIV
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