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Aggregation-induced emission magnetic fluorescent coding microsphere, preparation method and application thereof

A technology of aggregation-induced luminescence and fluorescence encoding, which is applied in the field of preparation of aggregation-induced luminescence magnetic fluorescent encoding microspheres, can solve problems that have not been seen yet, and achieve high stability, strong encoding ability, and good uniformity

Pending Publication Date: 2018-09-28
ZHEJIANG ORIENT GENE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Aggregation-induced luminescent material is a kind of coding material with excellent performance, but there is no report on its application in the preparation of fluorescent coding microspheres

Method used

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  • Aggregation-induced emission magnetic fluorescent coding microsphere, preparation method and application thereof
  • Aggregation-induced emission magnetic fluorescent coding microsphere, preparation method and application thereof
  • Aggregation-induced emission magnetic fluorescent coding microsphere, preparation method and application thereof

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Embodiment 1

[0025] figure 1 It is a preferred embodiment of the method for preparing aggregation-induced luminescence magnetic fluorescent coded microspheres provided by the present invention. The aggregation-induced luminescence magnetic fluorescent coded microspheres prepared in this example are HPS / Fe3O4 / PSMA magnetic fluorescent coded microspheres , The aggregation-inducing luminescent material is 1,1,2,3,4,5-hexaphenylsilole (HPS), the polymer is styrene-maleic anhydride copolymer (PSMA), and the preparation method is SPG film emulsification- Emulsion solvent evaporation method, the specific preparation steps are:

[0026] Dissolve 0.01 mg of 1,1,2,3,4,5-hexaphenyl silole (HPS) and 0.25 g of styrene-maleic anhydride copolymer (PSMA) in 4 mL of toluene to form a dispersed phase;

[0027] Dissolve 1 g of sodium dodecyl sulfate (SDS) in 200 mL of ultrapure water to form a continuous phase;

[0028] Using an SPG membrane with a pore size of 5μm, a uniform and stable oil-in-water emulsion can b...

Embodiment 2

[0032] A preferred embodiment of the method for preparing aggregation-induced luminescence magnetic fluorescent coded microspheres provided by the present invention. The aggregation-induced luminescence magnetic fluorescent coded microspheres prepared in this embodiment are HPS / Fe3O4 / PSMA magnetic fluorescent coded microspheres. The aggregation-inducing luminescent material is 1,1,2,3,4,5-hexaphenylsilole (HPS), the polymer is styrene-maleic anhydride copolymer (PSMA), and the preparation method is SPG film emulsification-emulsion Solvent evaporation method, the specific preparation steps are:

[0033] Dissolve 0.003mg of 1,1,2,3,4,5-hexaphenylsilole (HPS) and 0.25g of styrene-maleic anhydride copolymer (PSMA) in 4mL of toluene to form a dispersed phase;

[0034] Dissolve 1 g of sodium dodecyl sulfate (SDS) in 200 mL of ultrapure water to form a continuous phase;

[0035] Using an SPG membrane with a pore size of 3μm, a uniform and stable oil-in-water emulsion can be prepared under ...

Embodiment 3

[0039] figure 2 It is a preferred embodiment of the method for preparing aggregation-induced luminescence magnetic fluorescent coded microspheres provided by the present invention. The aggregation-induced luminescence magnetic fluorescent coded microspheres prepared in this example are TPE / Fe3O4 / MOTAS magnetic fluorescent coded microspheres , Where the aggregation-inducing luminescent material is tetrastyrene (TPE), the polymer is styrene-acrylic copolymer (MOTAS), the preparation method is SPG film emulsification-emulsion solvent evaporation method, the specific preparation steps are:

[0040] Dissolve 0.5 mg of tetrastyrene (TPE) and 0.25 g of styrene-acrylic acid copolymer (MOTAS) in 4 mL of toluene to form a dispersed phase;

[0041] Dissolve 1 g of sodium dodecyl sulfate (SDS) in 200 mL of ultrapure water to form a continuous phase;

[0042] Using an SPG membrane with a pore size of 5μm, a uniform and stable oil-in-water emulsion can be prepared under a nitrogen pressure of 3KP...

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Abstract

The invention discloses an aggregation-induced emission magnetic fluorescent coding microsphere. The coding microsphere comprises a polymer, an aggregation-induced emission material and magnetic nano-particles, the particle size of the coding microsphere is 0.3-20[mu]m, and the variation coefficient CV is less than 10%. The invention also discloses a preparation method of the aggregation-induced emission magnetic fluorescent coding microsphere, and discloses an application of the aggregation-induced emission magnetic fluorescent coding microsphere which can be used for detecting one or more target objects. The invention provides a fluorescent coding microsphere with high fluorescence intensity, high stability, good uniformity of fluorescent signals, strong coding capability and a large number of codes, and solves the problems of poor stability, poor anti-fluorescent bleaching capability, aggregation-caused quenching and the like of traditional organic fluorescent dyes, as well as the problems of re-absorption between fluorescent signals of different quantum dots and self-quenching under high concentration in a coding process of quantum dots, and the fluorescent coding microsphere has a very important significance and wide application prospect in liquid-phase biochip detection technology.

Description

Technical field [0001] The invention relates to the field of preparation and application of micro-nano materials, in particular to an aggregation-induced luminescence magnetic fluorescent coding microsphere, a preparation method and application thereof. Background technique [0002] The liquid-phase biochip technology based on coded microspheres developed in recent years is based on xMAP (flexiblemulti-analyte profiling) technology, which uses coded microspheres as the carrier of liquid phase reaction. According to the interaction rules of protein-protein, nucleic acid-nucleic acid, etc., Fast and high-throughput flow cytometry is used as an analysis method to perform rapid and high-throughput multiple quantitative detection of proteins and nucleic acids. The core technology is polymer microspheres with coding signals. Appropriate coded microspheres should have the following properties: good coding signal stability, good uniformity, large number of codes, and convenient decoding....

Claims

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Application Information

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IPC IPC(8): C09K11/06C09K11/02B01J13/12G01N21/64
CPCB01J13/125C09K11/025C09K11/06G01N21/6402
Inventor 李万万武卫杰
Owner ZHEJIANG ORIENT GENE BIOTECH
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