Preparation method and application of high temperature-resistant and salt-resistant kitchen waste decomposing compound bacteria agent

A compound bacterial agent and high-temperature-resistant technology, applied in the biological field, can solve the problems of narrow application range, unsatisfactory treatment effect, inability to survive and reproduce, etc., and achieve the effect of wide selection range, remarkable decomposing effect and strong application ability

Active Publication Date: 2018-09-28
黄山中科新佳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] 1. A stable micro-ecological system should be formed among the strains of the compound bacterial agent, and it can metabolize various substrates with comprehensive functions. Because the types of kitchen waste are complex, including sugar, protein, oil, cellulose, etc., if the bacteria The single function of the agent often has a narrow scope of application, and the treatment effect is not ideal
[0004] 2. The compound bacterial agent must be able to withstand high salt content, because the salt will accumulate during the process of food waste decomposing and dr

Method used

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  • Preparation method and application of high temperature-resistant and salt-resistant kitchen waste decomposing compound bacteria agent
  • Preparation method and application of high temperature-resistant and salt-resistant kitchen waste decomposing compound bacteria agent
  • Preparation method and application of high temperature-resistant and salt-resistant kitchen waste decomposing compound bacteria agent

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Effect test

Embodiment 1

[0059] Embodiment 1, high-temperature and salt-resistant kitchen decomposing compound bacterial agent, is made up of solid decomposing bacteria, composite bacterial liquid and enzyme preparation, and solid decomposing bacteria is made up of streptomyces sp. (Streptomyces sp.) and solid fermented material, and solid fermented material is A mixture of oats, rice bran, and bran; the compound bacterial solution is a mixture of Bacillus subtilis, Bacillus amyloliquefaciens, and Saccharomyces cerevisiae; the enzyme preparation is cellulase and protease , a mixture of amylase and lipase (the above enzymes are resistant to high temperature).

[0060] The preparation method of the high-temperature and salt-resistant kitchen decomposing composite bacterial agent (hereinafter referred to as composite bacterial agent) specifically comprises the following steps:

[0061] (1), preparation of solid decomposing bacteria:

[0062] Include the following steps:

[0063] (1.1), preparation meth...

Embodiment 2

[0083] Embodiment 2, the embodiment 1 step (1.2) oats, rice bran and bran are evenly mixed according to the ratio of 5:3:2 and changed to mix by volume ratio 3:1:4; Bacillus subtilis in step (2.4) The bacterial liquid, the Bacillus amyloliquefaciens bacterial liquid and the Saccharomyces cerevisiae bacterial liquid are mixed in a volume ratio of 6:3:1 to be mixed in a volume ratio of 3:6:1; and in step (3), cellulase, protease, The mixing ratio of amylase and lipase powder is changed from 4:2:2:2 to 1:4:4:1, and the rest are the same as in Example 1.

Embodiment 3

[0084] Embodiment 3, the embodiment 1 step (1.2) oats, rice bran and bran are evenly mixed according to the ratio of 5:3:2 and changed to mix by volume ratio 4:3:4; Bacillus subtilis in step (2.4) The bacterial liquid, the Bacillus amyloliquefaciens bacterial liquid and the Saccharomyces cerevisiae bacterial liquid are mixed in a volume ratio of 6:3:1 to be mixed in a volume ratio of 4:4:2; and in step (3), cellulase, protease, The mixing ratio of amylase and lipase powder is changed from 4:2:2:2 to 4:1:1:2, and the rest are the same as in Example 1.

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Abstract

The invention discloses a preparation method of a high temperature-resistant and salt-resistant kitchen waste decomposing compound bacteria agent. The preparation method comprises the following steps:respectively performing seed culture on streptomycete, bacillus subtilis, bacillus amyloliquefaciens and saccharomyces cerevisiae, so that a streptomycete mycelium pellet suspension, a bacillus subtilis bacteria solution, a bacillus amyloliquefaciens bacteria solution and a saccharomyces cerevisiae bacteria solution are respectively and correspondingly obtained; inoculating the streptomycete mycelium pellet suspension to a solid fermentation material for culture to obtain solid decomposing bacteria; mixing the other three bacteria solutions to obtain a compound bacteria solution; stirring anduniformly mixing the compound bacteria solution and the solid decomposing bacteria according to the proportion of 9-11 ml/100g, then drying at a low temperature, and finally adding an enzyme preparation for mixing to obtain the decomposing compound bacteria agent. The compound bacteria agent is used for decomposing kitchen wastes.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method and application of a high-temperature and salt-resistant kitchen decomposing compound bacterial agent. Background technique [0002] Organic material decomposing bacteria agents, especially those used for food waste treatment, need to overcome the following problems: [0003] 1. A stable micro-ecological system should be formed among the strains of the compound bacterial agent, and it can metabolize various substrates with comprehensive functions. Because the types of kitchen waste are complex, including sugar, protein, oil, cellulose, etc., if the bacteria The single function of the agent often has a narrow scope of application, and the treatment effect is not ideal. [0004] 2. The compound bacterial agent must be able to withstand high salt content, because the salt will accumulate during the process of food waste decomposing and drying in the processing cabi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N9/20C12N9/26C12N9/42C12N9/50B09B3/00C12R1/465C12R1/125C12R1/07C12R1/865
CPCB09B3/00C12N1/18C12N1/20C12N9/20C12N9/2411C12N9/2437C12N9/50C12Y301/01003C12Y302/01004
Inventor 李光超罗小艳王道胜
Owner 黄山中科新佳生物科技有限公司
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