Application of carbon fluorescent quantum dots in inhibiting Escherichia coli biofilm formation
A technology based on carbon fluorescent quantum dots and Escherichia coli, which is applied in the direction of microorganism-based methods, chemicals for biological control, food ingredients as anti-microbial preservation, etc., which can solve poor biocompatibility, complicated steps, unsuitable for industrialization, etc. question
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Embodiment 1
[0030] The preparation of embodiment 1 carbon fluorescent quantum dot (CDs-LP):
[0031] Lactobacillus plantarum LCC-605 preserved in glycerol tubes was inoculated into liquid MRS medium at a ratio of 5%, and cultured statically at 37°C for 24 hours, and the obtained fermentation broth was centrifuged at 8000rpm for 15 minutes to obtain bacterial cells for use. Suspend the above bacterium in 50mL water and place it in a 150mL hydrothermal reaction kettle. Put it in an oven at 240°C and react for 48h. After the reaction, the residue was removed, and the large particles were removed by centrifugation at 10,000 rpm for 12 minutes, and the remaining liquid was filtered with a 0.35 μm filter membrane to obtain carbon fluorescent quantum dots with multicolor luminescence and other properties.
Embodiment 2
[0032] The preparation of embodiment 2 carbon fluorescent quantum dots (CDs-LP):
[0033] Lactobacillus plantarum LCC-605 preserved in a glycerol tube was inoculated in a liquid MRS medium at a ratio of 1%, placed at 25°C for static culture for 12 hours, and the obtained fermentation broth was centrifuged at 5000rpm for 15 minutes to obtain bacterial cells for use. Take the above bacteria and suspend them in 20mL of water, and place them in a 100mL hydrothermal reaction kettle. Put it in an oven at 120°C and react for 18h. After the reaction, the residue was removed, and the large particles were removed by centrifugation at 8000 rpm for 15 minutes, and the remaining liquid was filtered with a 0.45 μm filter membrane to obtain carbon fluorescent quantum dots with multicolor luminescence and other properties.
Embodiment 3
[0034] The preparation of embodiment 3 carbon fluorescent quantum dots (CDs-LP):
[0035] Lactobacillus plantarum LCC-605 preserved in a glycerol tube was inoculated at a ratio of 3% in liquid MRS medium, placed at 31°C for static culture for 12 hours, and the obtained fermentation broth was centrifuged at 5000rpm for 10 minutes to obtain bacterial cells for use. Suspend the above-mentioned bacterium in 30mL water, and place it in a 100mL hydrothermal reaction kettle. Put it in an oven at 120°C and react for 12h. After the reaction, remove the residue, centrifuge at 12,000 rpm for 10 min to remove large particles, and filter the remaining liquid with a 0.22 μm filter membrane for later use.
[0036] The transmission electron microscope observation of the carbon fluorescent quantum dot that above-mentioned preparation method obtains:
[0037] The filtered carbon quantum dots were diluted 10 times with deionized water, and 10 μL was dropped on a 400-mesh copper grid, and obser...
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