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Electrostatic spinning method for extracellular matrix and electrostatic spinning fiber

An electrospinning and exogenous matrix technology, applied in the direction of electrospinning, fiber treatment, textiles and papermaking, etc., can solve the problems of electrospinning that cannot remove the cell matrix, and achieve the advantages of cell attachment, more bioactive components, and natural high degree of effect

Inactive Publication Date: 2018-10-12
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The invention provides an electrospinning method of extracellular matrix, aiming to improve the problem that the existing electrospinning technology cannot use decellularized matrix as the main material for electrospinning

Method used

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  • Electrostatic spinning method for extracellular matrix and electrostatic spinning fiber
  • Electrostatic spinning method for extracellular matrix and electrostatic spinning fiber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The extracellular matrix that has been decellularized is powdered with a powder machine. After the powder is finished, it is passed through a 30-mesh sieve to obtain an extracellular matrix powder with a relatively uniform particle size. The extracellular matrix selected in this example is pig-derived small intestinal mucosa The underlying extracellular matrix.

[0045] After sieving, weigh 3g of extracellular matrix powder, and measure 100mL of trifluoroethanol, and add the extracellular matrix powder into trifluoroethanol, mix the two to obtain a mixed solution, and stir the mixed solution for 2 days , so that the soluble powder in the extracellular matrix is ​​fully dissolved in the solution, and the insoluble powder is evenly suspended in the solution.

[0046] After the stirring, the mixed solution was placed in a 2mL centrifuge tube, steel balls were added, and then the centrifuge tube was put into a ball mill for ball milling for 10 min at -8°C and a frequency of...

Embodiment 2

[0050] The extracellular matrix that has been decellularized is powdered with a powder machine, and after the powder is finished, it is passed through a 50-mesh sieve to obtain an extracellular matrix powder with a relatively uniform particle size. The extracellular matrix selected in this example is pig-derived small intestinal mucosa The underlying extracellular matrix.

[0051] After sieving, weigh 4g of extracellular matrix powder, and measure 100mL of hexafluoroisopropanol, add the extracellular matrix powder into hexafluoroisopropanol, mix the two to obtain a mixed solution, and stir the mixed solution The time is 7 days, so that the soluble powder in the extracellular matrix is ​​fully dissolved in the solution, and the insoluble powder is uniformly suspended in the solution.

[0052] After the stirring, put the mixed solution into a 2mL centrifuge tube, add zirconia pellets, then put the centrifuge tube into a ball mill and mill for 60min at -12°C and 80HZ frequency. ...

Embodiment 3

[0056] The extracellular matrix that has been decellularized is powdered with a powder machine, and after the powder is finished, it is passed through a 40-mesh sieve to obtain an extracellular matrix powder with a relatively uniform particle size. The extracellular matrix selected in this example is porcine-derived peripheral nerve extracellular matrix.

[0057] After sieving, weigh 3.2g of extracellular matrix powder, and measure 100mL of hexafluoroisopropanol, and add the extracellular matrix powder into hexafluoroisopropanol, mix the two to obtain a mixed solution, and stir the mixed solution The stirring time is 4 days, so that the soluble powder in the extracellular matrix is ​​fully dissolved in the solution, and the insoluble powder is evenly suspended in the solution.

[0058] After the stirring, put the mixed solution into a 2mL centrifuge tube, add zirconia pellets, and then put the centrifuge tube into a ball mill for 20min at -11°C and 60HZ. Thoroughly break up t...

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Abstract

The invention relates to the technical field of electrostatic spinning, and provides an electrostatic spinning method for an extracellular matrix. The method comprises the operation steps that extracellular matrix powder with no cell and a fluorine-containing high-polarity solvent are mixed on the basis that the ratio is 0.03-0.04 g:1 mL, and a mixed solution is obtained; the mixed solution with no high polymer material is adopted as a spinning solution for electrostatic spinning to obtain electrostatic spinning fiber. The fiber obtained through the method has the performance of micro-nano multi-grade fiber, and due to the fact that no high polymer material is added into the spinning solution, cell adhesion, differentiation and functionalization are facilitated. The invention further provides electrostatic spinning fiber manufactured through the method, the fiber has the performance of the micro-nano multi-grade fiber, the fiber diameter distribution is wide, the fiber natural degree is high, and cell adhesion, differentiation and functionalization are facilitated.

Description

technical field [0001] The invention relates to the technical field of electrospinning, in particular to an electrospinning method of extracellular matrix and an electrospinning fiber. Background technique [0002] Tissue engineering (tissue engineering) is based on cell biology and material science, a multidisciplinary emerging science, and conducts research on the construction of tissues or organs in vitro or in vivo. Tissue engineering has proposed a new idea for tissue damage repair. The core of tissue engineering is to construct tissue engineering scaffolds with good biocompatibility to provide good carriers for cell transplantation and damage repair. [0003] The human body's natural tissue extracellular matrix has a nanofiber structure, which is conducive to the attachment of cells and can provide cells with physical signals to guide their functionalization. Based on bionic considerations, it is necessary for tissue engineering scaffolds to mimic the nanofibrous str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01D5/00
CPCD01D5/0007D01D5/003D01D5/0061
Inventor 全大萍杜钊夷陈诗浩孟月冯文娟饶子龙蔡绮幸
Owner SUN YAT SEN UNIV
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