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Phenothiazine derivative fluorescence carbon dots as well as preparation method and application thereof

A technology of phenothiazine derivatives and fluorescent carbon dots, applied in photodynamic therapy, chemical instruments and methods, nano-carbon, etc., can solve the problems of carcinogenic risk and DNA infection

Active Publication Date: 2018-10-19
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, phenothiazine dyes can infect DNA and cause cancer to the human body, so it is necessary to develop a safe and effective phenothiazine fluorescent carbon dot

Method used

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  • Phenothiazine derivative fluorescence carbon dots as well as preparation method and application thereof
  • Phenothiazine derivative fluorescence carbon dots as well as preparation method and application thereof
  • Phenothiazine derivative fluorescence carbon dots as well as preparation method and application thereof

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preparation example Construction

[0029] In a further specific embodiment, the preparation method of the present invention includes the following steps:

[0030] (1) preparing an aqueous solution of a phenothiazine derivative with a concentration of 0.5-5 mg / mL;

[0031] (2) Add passivating agent to the solution prepared in step (1), naturally cool to room temperature after hydrothermal reaction;

[0032] (3) The product obtained in step (2) is centrifuged, and the obtained supernatant is processed to obtain fluorescent carbon dots of phenothiazine derivatives.

[0033] Wherein, the passivating agent described in step (2) is 1-30 mg / mL PEG200-2000 or 0.1-5 mg / mL urea. In this description, the concentration of the passivating agent represents the concentration of the passivating agent in the reaction system.

[0034] On the other hand, step (2) refers to the hydrothermal reaction of phenothiazine derivatives in the presence of a passivating agent, and the conditions of this reaction can be defined according t...

Embodiment 1

[0038] Preparation of methylene blue carbon dots: Weigh 15 mg of methylene blue into a 25 mL beaker, add 15 mL of deionized water and stir to dissolve it, then add 150 μL PEG800 into it with a pipette gun, and stir evenly. The resulting solution was transferred into a 50ml reactor at 180°C for 12 hours. After the reaction, cool to room temperature in the air, centrifuge at 8000r / min for 20 minutes to obtain the methylene blue carbon dot solution, store it at around 4°C, and use it for the following performance test experiments 1-5.

[0039] (1) Performance Test Experiment 1: Morphology analysis of methylene blue carbon dots.

[0040] The methylene blue carbon quantum dot solution was taken, and the hydrated average particle size was measured by a Zeta potential and particle size analyzer to be 4.3nm, and the Zeta potential was -7.3V. Obtain methylene blue carbon dot TEM images (such as figure 1 ). figure 1 In the middle, the scale bar of the large picture is 20nm, and the i...

Embodiment 2

[0054] Preparation of toluidine blue (CAS registration number: 92-31-9) carbon dots: Weigh 15 mg of toluidine blue and place it in a 25 mL beaker, add 15 mL of deionized water and stir to dissolve it, then measure it with a pipette gun 200μL PEG800 was added and stirred evenly. The resulting solution was transferred into a 50ml reactor at 160°C for 8 hours of reaction. After the reaction, cool to room temperature in the air, and centrifuge at a speed of 8000r / min for 20 minutes to obtain a toluidine blue carbon dot solution, which is stored at about 4°C until use.

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Abstract

The invention discloses phenothiazine derivative fluorescence carbon dots as well as a preparation method and application thereof. The preparation method comprises a step of hydrothermal synthesis reaction, reaction raw materials are prepared from a phenothiazine derivative and a passivating agent; the phenothiazine derivative has a structure shown in a general formula I. The method has the advantages of simple, economic, green and environment-friendly synthesis process; the prepared phenothiazine derivative fluorescence carbon dots have the advantages of uniform particle size and stable structure, and have obvious adsorption and fluorescence emission in the visible light and near-infrared areas. The carbon dots can be used for cell fluorescence imaging, and singlet oxygen can be producedunder light irradiation of visible light and near-infrared wavelength and the carbon dots can be used for killing tumor cells, and the carbon dots are desired photosensitizers for photodynamic therapy. The formula (1) is shown in the description.

Description

technical field [0001] The invention relates to carbon nanomaterials, a preparation method thereof, and photodynamic therapy, in particular to a method for preparing fluorescent carbon dots using phenothiazine derivatives as raw materials and the prepared fluorescent carbon dots. technical background [0002] Carbon dots are a new type of carbon nanomaterial with a size of less than 10nm, which can emit fluorescence under ultraviolet light, and have excellent properties such as good water solubility, biocompatibility, low toxicity, photobleaching resistance, and photostability. Therefore, it has broad application prospects in the fields of bioimaging, fluorescent labeling, biosensing, and metal ion detection. [0003] Photodynamic therapy (Photodynamic Therapy, PDT) is an emerging cancer treatment method. When the tumor site is irradiated with light of a specific wavelength, the photosensitizer accumulated in the tumor tissue will be excited to an excited state, and then th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B32/15C09K11/65B82Y20/00A61K41/00A61P35/00
CPCA61K41/0057A61P35/00B82Y20/00C09K11/65C01B32/15
Inventor 杜健军谷泉泳樊江莉彭孝军
Owner DALIAN UNIV OF TECH
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