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Method for comprehensively utilizing waste tobacco leaves

A technology of waste tobacco leaves and tobacco leaves, applied in the field of bioengineering, can solve the problems of insufficient utilization of active ingredients, low nicotine purity, high nicotine content, etc., and achieve the effects of low cost, short production cycle, and high number of viable bacteria

Active Publication Date: 2018-10-23
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to provide a method for simultaneously preparing high-purity nicotine, solanesol, spices, rutin, chlorogenic acid and microbial fertilizer by using waste tobacco leaves, so as to solve the problem of insufficient utilization of active ingredients in the existing method, complex process, Low yield, high content of nicotine in spice extract, low purity of nicotine, solanesol, spices, rutin and chlorogenic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] (2) Preparation of spice extract and solanesol: Preparation of spice extract and solanesol: remove the ethanol in the extract, adjust the pH to 3, extract with petroleum ether, and extract for 3 times to obtain the extract I and raffinate I; the extract I was concentrated, dehydrated, distilled, and dried to obtain the crude spice extract; the crude spice extract was dissolved in 90% aqueous ethanol, chromatographed with a macroporous resin, and used 100% Elute with ethanol solution, combine and concentrate the flow-through components to dryness to obtain the essence of spice extract, combine and concentrate the eluted components to dryness, dissolve in ethanol, methanol, acetone or acetonitrile, and crystallize at 4°C to obtain solanesol;

[0032] (3) Preparation of rutin: after concentrating the raffinate I into 50% of the original volume, let it stand overnight, separate out the precipitate, and collect the precipitate by centrifugation or filtration; and recrystalliz...

Embodiment 1

[0055] Example 1. Determination of content of nicotine, solanesol, chlorogenic acid and flavor components in tobacco leaves

[0056] Weigh 10g of tobacco leaf sample, carry out Soxhlet extraction, extraction solvent is 70ml 90% ethanol, extraction temperature is 70 ℃, extraction time is 4 hours. Three experiments were carried out in parallel, and the average nicotine content measured was 31.5 mg / g tobacco leaves, the content of chlorogenic acid was 6.66 mg / g tobacco leaves, the neophytadiene content was 1.46 mg / g tobacco leaves, and the rutin content was 5.0 mg / g tobacco leaves. g tobacco leaves, add NaOH to the Soxhlet extract to a final concentration of 0.3M and saponify at 70°C for 30min, the content of solanesol in the saponified solution is 9.1mg / g tobacco leaves, nicotine, chlorogenic acid, solanesol, reed The contents of butyl and neophytadiene were 3.15%, 0.66%, 0.91%, 0.50% and 0.15%, respectively.

Embodiment 2

[0057] Embodiment 2. Preparation 1 of the crude extract of spices

[0058] Weigh 60g of tobacco leaf samples into a 1L round-bottomed three-neck flask, add 600mL of 90% ethanol aqueous solution, heat reflux at 50°C for 30 minutes, extract 3 times in total, and combine to obtain the reflux liquid and residue. GC and HPLC analysis showed that in the reflux liquid, the extraction yields of nicotine, solanesol, chlorogenic acid, chlorogenic acid, neophytadiene and rutin were 87.5%, 61.9%, 95.9%, 93.5%, respectively, 91.1% and 92%. The reflux solution was evaporated with a vacuum rotary evaporator to remove ethanol at a water bath temperature of 35°C, adjusted to pH 2 with dilute sulfuric acid, and extracted three times with an equal volume of petroleum ether to obtain petroleum ether extract and raffinate I, combined with petroleum ether for extraction After liquid, concentrate under reduced pressure in a 35°C water bath, and dry to obtain the spice extract. GC and HPLC analysis...

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PUM

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Abstract

The invention discloses a method for comprehensively utilizing waste tobacco leaves. The method comprises the steps of after using an organic solvent to extract the waste tobacco leaves, using a stepwise solvent extraction method to obtain condiments, chlorogenic acid, and precipitates of a nicotine extract and rutin; obtaining high-purity nicotine, condiments, solanesol, rutin and chlorogenic acid by using separation and purification means of solvent extraction, column chromatography, crystallization and the like; fermenting tobacco leaf residues of the extracted effective components to prepare a bacterial manure. The method provided by the invention can realize high-value utilization of effective components of multiple waste tobacoo leaves, is simple in process and low in cost, and meetsthe large-scale industrial production requirement; the yield and the purity of the effective components are high, the condiments do not contain the nicotine, and microbial fertilizer is short in production period and high in viable count, so that the method is a tobacco leaf comprehensive utilization technology with high efficiency, good economy and environment friendliness.

Description

technical field [0001] The invention relates to a method for comprehensive utilization of effective components in tobacco leaves, which can simultaneously prepare nicotine, solanesol, spices, rutin, chlorogenic acid and microbial fertilizer, and belongs to the field of bioengineering. Background technique [0002] my country's tobacco planting area and output are among the top in the world, but due to the limitations of planting management and processing technology, about 25% of tobacco leaves and leftovers are not fully utilized and discarded in the process of cultivation, transportation and cigarette making. Burning these waste tobacco leaves or discarding them at will is not only a waste, but also pollutes the environment. After research, more than 300 compounds have been identified in tobacco leaves, among which important compounds such as nicotine, solanesol, spices, rutin and chlorogenic acid are the main components in tobacco leaves, which are widely used in chemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C33/02C07C29/74C07C29/86C07C29/76C07C69/732C07C67/48C07C67/58C07H17/07C07H1/08C07D401/04C11B1/10C11B9/02
CPCC07C29/74C07C29/76C07C29/86C07C67/48C07C67/58C07D401/04C07H1/08C07H17/07C11B1/10C11B9/022C07C33/02C07C69/732
Inventor 董悦生王月李野修志龙戴建英
Owner DALIAN UNIV OF TECH
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