Method for separating amoxicillin from reaction products of enzymatic preparation of amoxicillin
A technology of amoxicillin and reaction products, which is applied in the field of preparation of amoxicillin, can solve the problems of poor fluidity and low product yield, and achieve the effects of good fluidity, improved product yield, and improved product quality
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Embodiment 1
[0054] This example is used to illustrate the method for separating amoxicillin from the reaction product of enzymatic preparation of amoxicillin provided by the present invention
[0055] (1) Add 70 g of immobilized penicillin acylase, 50 g of 6-APA, 49 g of p-hydroxyphenylglycine methyl ester, and 800 ml of purified water into the enzyme reactor, stir evenly, control the temperature at 20.0° C., and start the reaction. When the pH value stabilized and the 6-APA dropped to 1.0 mg / ml, it was judged that the reaction was over.
[0056] (2) Cool the reaction product to 6°C and separate the enzyme through a 80-mesh sieve. After the separation, wash the enzyme with 150ml of purified water pre-cooled to about 6°C for 3 times, and pass each enzyme washing water through an 80-mesh sieve. net to obtain the enzyme washing solution. Mix the reaction solution and the enzyme washing solution evenly, then transfer it to a dissolution tank, add 3N hydrochloric acid to dissolve it, and cont...
Embodiment 2
[0061] This example is used to illustrate the method for separating amoxicillin from the reaction product of enzymatic preparation of amoxicillin provided by the present invention
[0062] (1) Add 70 g of immobilized penicillin acylase, 50 g of 6-APA, 54 g of p-hydroxyphenylglycine methyl ester hydrochloride, and 800 ml of purified water into the enzyme reactor, stir evenly, control the temperature at 20.0° C., and start the reaction. When the pH value stabilized and the 6-APA dropped to 1.0 mg / ml, it was judged that the reaction was over.
[0063] (2) Cool the reaction product to 3°C, and separate the enzyme through a 80-mesh sieve. After the separation, wash the enzyme with 150ml of purified water pre-cooled to about 3°C for 3 times, and pass each enzyme washing water through an 80-mesh sieve. net to obtain the enzyme washing solution. Mix the reaction solution and the enzyme washing solution evenly, then transfer it to a dissolving tank, add 3N nitric acid to dissolve it...
Embodiment 3
[0068] This example is used to illustrate the method for separating amoxicillin from the reaction product of enzymatic preparation of amoxicillin provided by the present invention
[0069] (1) Add 70 g of immobilized penicillin acylase, 50 g of 6-APA, 49 g of p-hydroxyphenylglycine methyl ester, and 800 ml of purified water into the enzyme reactor, stir evenly, control the temperature at 20.0° C., and start the reaction. When the pH value stabilized and the 6-APA dropped to 1.0 mg / ml, it was judged that the reaction was over.
[0070] (2) Cool the reaction product to 1°C, and separate the enzyme through a 80-mesh sieve. After the separation, wash the enzyme with 150ml of purified water pre-cooled to about 1°C for 3 times, and pass each enzyme washing water through an 80-mesh sieve. net to obtain the enzyme washing solution. Mix the reaction solution and the enzyme washing solution evenly, then transfer it to a dissolving tank, add 6N hydrochloric acid to dissolve it, and cont...
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