Preparation method and application of photoelectric biosensor based on oxide nanoarray

A biosensor and nanoarray technology, applied in the fields of instruments, scientific instruments, material electrochemical variables, etc., can solve the problems of affecting the accuracy of detection results, low dye modification efficiency, and many non-specific adsorption, and achieve easy promotion and construction. Simple, affordable results

Active Publication Date: 2020-11-27
BEIJING CENT FOR PHYSICAL & CHEM ANALYSIS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has a relatively good detection effect, its operation process is relatively complicated, requiring high-temperature calcination, dye embedding and other links
There are disadvantages such as long time-consuming, low dye modification efficiency, and more non-specific adsorption, so it not only affects the accuracy of the detection effect, but also has certain risks

Method used

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  • Preparation method and application of photoelectric biosensor based on oxide nanoarray
  • Preparation method and application of photoelectric biosensor based on oxide nanoarray
  • Preparation method and application of photoelectric biosensor based on oxide nanoarray

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preparation example Construction

[0063] In a preferred embodiment of the present invention, the present invention provides a method for preparing an oxide nanoarray, which includes the following steps: pretreating ITO or FTO glass, and then planting the seed solution on the ITO or FTO glass, Next, the ITO or FTO glass containing the seeds is placed in the growth solution corresponding to the oxide to continue growing, so as to obtain the oxide nano-array electrode.

[0064] In a preferred specific embodiment of the present invention, the present invention provides a method for preparing a DNA-modified noble metal nanoparticle probe, which comprises the following steps: DNA1 and DNA2 are hybridized, and then the hybridized DNA double The strands are added to the noble metal nanoparticle solution, and centrifuged to obtain DNA-modified noble metal nanoparticle probes.

[0065] When adopting the photoelectric biosensor prepared by the present invention to carry out the test, the light source used in the photoele...

Embodiment 1

[0072] Example 1 Preparation of photoelectric biosensor

[0073] (1) Synthesis of DNA-modified gold nanoprobes (DNA@AuNPs):

[0074] a. 100ml of 0.01% (w / v) chloroauric acid solution was stirred and heated to boiling, then 1% (w / v) sodium citrate solution was quickly added to the chloroauric acid solution, when the solution turned wine red, stop Heating and cooling to obtain gold nanoparticles (AuNPs);

[0075] b. DNA1: 5′-SH-C6-ATCGTTTAGGATTTGGATGA-P-3′ and DNA2: 3′-GCAAATCCTAAAC are respectively configured into 10 -6 M solution, hybridization incubation at 37°C, after the reaction is complete, add the hybridized DNA double strands into 1mL nano-gold solution, stir at room temperature for 24h, slowly add 150μL 1M sodium chloride solution, and place it for 24h, The mixed solution was centrifuged at 10,000 rpm for 15 min, and the supernatant was skimmed off to obtain DNA@AuNPs.

[0076] (2) Preparation of zinc oxide nanoarrays

[0077] The ITO glass was ultrasonically clean...

Embodiment 2

[0089] Example 2 Preparation of photoelectric biosensor

[0090] (1) The synthesis operation of DNA-modified gold nano-probes (DNA@AuNPs) is the same as in Example 1;

[0091] (2) Preparation of titania nanoarrays

[0092] The FTO glass was ultrasonically cleaned in acetone, sodium hydroxide (1M) ethanol water (1:1v / v) solution, and water for 15 minutes, then placed in an oven at 90°C for 12 hours, and dried for later use;

[0093] With 35mL of diluted concentrated hydrochloric acid (concentrated hydrochloric acid: water = 1:1) as a solvent, add 1mL of tetrabutyl titanate dropwise under the condition of magnetic stirring, stir for 20min and then add it into a polytetrafluoroethylene reaction kettle, FTO The glass was placed vertically against the wall in a polytetrafluoroethylene reactor, and then the reactor was placed in a vacuum box at 150°C for 4 hours, and then the electrode was taken out, washed with water, and dried at 60°C to obtain a titanium oxide nano-array electro...

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Abstract

The invention relates to the field of material and analytical chemistry, and concretely relates to a photoelectric biosensor based on an oxide nano array material and a preparation method and an application thereof. The photoelectric biological sensor comprises a substrate, an oxide nano array electrode arranged at the substrate, a polypeptide layer arranged at the electrode, and a DNA-modified precious metal nanoparticle probe connected with the polypeptide layer, wherein the probe is connected with polypeptide through zirconium ions in a coordination mode. The method uses the one-dimensionalsemiconductor oxide nano array material as the substrate electrode to construct the photoelectric biological sensor, the photoelectric biological sensor is used for detecting protein kinase activity,the semiconductor oxide nano array has the characteristics of simple and safe synthesis, high specific surface area, and fast electron migration rate, the photoelectric biological sensor has the advantages of simple construction, economy and easy acquisition, safety, and easy popularization.

Description

technical field [0001] The invention relates to the field of materials and analytical chemistry, in particular to a photoelectric biosensor based on an oxide nano-array material and its preparation method and application. Background technique [0002] One-dimensional semiconductor oxide nanoarrays have large aspect ratio, large specific surface area, excellent mechanical length, excellent photoelectric properties and hot spot properties, etc. Wide range of applications. Since semiconductor oxide nanoarray materials can also effectively transport carriers, and have good biocompatibility and excellent stability, they also have great development potential in the field of biosensing. However, titanium oxide, zinc oxide, tin oxide, etc. are semiconductor materials with wide bandgap, which require high-energy ultraviolet light to be excited. Ultraviolet rays will affect the activity of biomolecules, thereby affecting the accuracy of experimental results. Therefore, it is necessa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/327
CPCG01N27/26G01N27/3278
Inventor 闫志勇邓平晔邹涛杨超杰
Owner BEIJING CENT FOR PHYSICAL & CHEM ANALYSIS
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