Unlock instant, AI-driven research and patent intelligence for your innovation.

Immunofluorescent chromatographic test strip for copper ion detection and application thereof

An immunofluorescence and chromatography test paper technology, applied in measurement devices, analytical materials, instruments, etc., can solve the problems of difficult to adapt to environmental pollution events, environmental quality screening and detection, long analysis time, low efficiency, etc., to achieve rapid and efficient detection, High sensitivity and easy to use effect

Inactive Publication Date: 2018-10-30
JINAN UNIVERSITY
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditional heavy metal detection methods mostly use physical / chemical methods, such as atomic absorption spectrometry (AAS), atomic fluorescence spectrometry (AFS), inductively coupled plasma spectrometry (ICP), etc., although the detection results are accurate and reliable , but requires large-scale equipment and professional operation, long analysis time, high cost, limited time and place, difficult to adapt to sudden environmental pollution incidents or large-scale environmental quality screening and detection
Existing detection methods for heavy metal copper are high in cost, low in efficiency and poor in accuracy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunofluorescent chromatographic test strip for copper ion detection and application thereof
  • Immunofluorescent chromatographic test strip for copper ion detection and application thereof
  • Immunofluorescent chromatographic test strip for copper ion detection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The preparation of embodiment 1 immunofluorescence chromatography test strip

[0053] Preparation and Identification of Complete Antigen for Copper Ion Detection

[0054] Coupling of Cu ions to the carrier protein OVA via the bifunctional chelator p-SCN-Bn-NOTA

[0055] Prepare pH8.9, 0.1M HEPES buffer solution, dissolve OVA protein powder in HEPES buffer solution to obtain OVA protein solution with a concentration of 10mg / ml; dissolve NOTA powder in DMSO solution to obtain NOTA with a concentration of 25mg / ml solution; add the NOTA solution dropwise to the OVA protein solution, stir and chelate in the ice box for 3 hours, and prepare the NOTA-OVA solution overnight at 4°C (the molar ratio of NOTA to OVA is about 70:1);

[0056] CuSO 4 ·5H 2 O powder was dissolved in ultrapure water to obtain a copper ion solution with a concentration of 1.0 mg / ml; the Cu 2+ The solution was added dropwise to the NOTA-OVA solution (Cu 2+ The molar ratio of Cu-NOTA-OVA to NOTA is ab...

Embodiment 2

[0067] The performance evaluation of the copper ion concentration in the detection sample with the immunofluorescence chromatography test strip in embodiment 1

[0068] Preparation of the standard curve: add a series of gradient concentrations (0, 0.8, 1.6, 3.12, 6.25, 12.5, 25, 50, 100ng / ml) competitor standard substance Cu-NOTA dropwise to 75ul per card. After 15 minutes, read the fluorescent signal on the machine. For each detection, 10 parallel groups were used to obtain the mean value, and the standard curve and inhibition rate (sensitivity) were calculated. Set the fluorescent signal T / C value of 0ng / ml standard well as B 0 , the T / C value of other wells is B, to detect the inhibition rate (B 0 -B) / B 0 ×100% is the ordinate, with Cu2+ Concentration log10 logarithm as the abscissa, draw the standard inhibition curve.

[0069] The photo after detecting copper ion in described immunofluorescence chromatography test strip product standard curve preparation process is as ...

Embodiment 3

[0097] Utilize the immunofluorescence chromatography test strip described in embodiment 1 to detect copper ion concentration in Chinese herbal medicine sample, soil sample, and water sample

[0098] 1. Microwave digestion recovery rate of Chinese herbal medicine and soil samples

[0099] Conditions of microwave digestion Accurately weigh 0.2000g of the sample, add 8ml of nitric acid, and pre-digest in a fume hood overnight or preheat the acid catcher at 130°C for 30 minutes; select the digestion program (climb at 130°C for 10 minutes, hold at 130°C for 3 minutes; ℃ for 5 minutes; 180 ℃ for 3 minutes, 180 ℃ for 40 minutes; cooling); the specific operation of coupling with the chelating agent after the microwave digestion of the sample described in the present invention: after the Chinese herbal medicine sample is digested, use a 10ml volumetric flask to make up the volume, and take 5ml for digestion Add 1-2 μl of 5 μg / μl single NOTA solution to the solution, mix and react for 1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides an immunofluorescent chromatographic test strip for copper ion detection and application of the immunofluorescent chromatographic test strip in detection of copper ion concentration in a sample, and belongs to the technical field of copper ion detection. The immunofluorescent chromatographic test strip comprises a glass fiber pad, a nitrocellulose membrane and absorbent paper which are lapped sequentially, a copper ion monoclonal antibody-fluorescent microsphere compound is sprayed on the glass fiber pad, and a detection line coated with a copper ion detection complete antigen and a quality control line coated with a second antibody are sequentially arranged on the nitrocellulose membrane. The immunofluorescent chromatographic test strip is convenient to use and highin sensitivity, the detection sensitivity (IC50) is 3.6ng / ml, the detection range (IC20-IC80) is 0.4-32.3ng / ml, and the detection limit (IC10) is 0.2ng / ml.

Description

technical field [0001] The invention belongs to the technical field of heavy metal immunodetection, and in particular relates to an immunofluorescence chromatography test strip for detecting copper ions and an application thereof. Background technique [0002] Heavy metals are widely distributed on the earth and will not cause harm to human living environment under normal circumstances. However, with the rapid expansion of cities and the rapid development of industries, a large amount of heavy metals have entered our living environment at an abnormal rate due to human activities. Or food chain enrichment and other ways seriously endanger the health of humans and animals. [0003] At present, the most serious heavy metals for environmental pollution include: lead, cadmium, mercury, chromium, arsenic (known as "five poisons"), in addition, it also includes nickel, zinc, copper, cobalt, tin, vanadium with relatively weak toxicity Wait. Although the toxicity of heavy metal co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/533
CPCG01N33/533G01N33/558G01N33/577
Inventor 向军俭
Owner JINAN UNIVERSITY