Pure white black fungus strain and application thereof

A technology of black fungus and bacterial strains, applied in the fields of application, fungi, fungal products, etc., can solve the problems of hard texture, low foaming rate, and low temperature resistance, and achieve good water absorption, improved texture, and high foaming rate Effect

Active Publication Date: 2018-11-02
泌阳县玉润农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the jade fungus still cannot change the following defects: (1) The texture is hard and brittle, without the texture of black fungus, and the taste is not as good as that of black fungus; (2) The back hair is long and not smooth enough
(3) The foaming rate is low, the foaming rate is only about 1:9
(4) Not resistant to low temperature

Method used

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  • Pure white black fungus strain and application thereof
  • Pure white black fungus strain and application thereof
  • Pure white black fungus strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Embodiment 1 pure white black fungus JAUH-W-591 cultivation method of the present invention

[0045] (1) Production of mother species, the mother species medium of pure white black fungus JAUH-W-591 is PDA medium: 200g of peeled potatoes, 20g of glucose, 20g of agar, 1000mL of water, pH=6.5~6.7, the peeled Potato cut 1cm 3 See square pieces, boil in boiling water for 15-20min, filter with 6 layers of gauze, boil the filtrate again, add agar and keep stirring, add glucose after the agar is completely dissolved, set the volume to 1000mL, put it into a test tube or Erlenmeyer flask, Rubber stoppers or cotton plugs are sealed and covered with aluminum foil, sterilized at 121°C for 30 minutes under high pressure, cooled to about 25°C, inserted into the ultra-clean workbench with JAUH-W-591 strains, and then placed in a 25°C constant temperature incubator. Cultivate in dark light for about 12 days, and the mother species can grow well;

[0046] (2) Pure white black fungus J...

experiment example 1

[0054] Experimental Example 1 Separation and purification of mutant strain "JAUH-W-01"

[0055] The separation and purification process of the white black fungus mutant strain "JAUH-W-01" of the present invention comprises the following steps:

[0056] 1) Natural drying white black fungus mutant strain, the specimens are preserved in the Engineering Research Center of Edible Fungus Ministry of Education, Jilin Agricultural University;

[0057] 2) Preparation of culture medium

[0058] PDA medium: 200g peeled potato, 20g glucose, 20g agar, 1000ml water, pH=6.5~6.7, cut the peeled potato into 1cm 3 See square pieces, boil in boiling water for 15-20min, then filter with 6 layers of gauze, boil the filtrate again, add agar and keep stirring, add glucose after the agar is completely dissolved, and dilute to 1000ml. Put it into a test tube or a Erlenmeyer flask, seal it with a rubber stopper or cotton stopper and cover it with aluminum foil. Sterilize under high pressure at 121°C...

experiment example 2

[0061] Experimental example 2 Isolation and purification of white black fungus strain "JAUH-W-02"

[0062] 1) Preparation of liquid culture medium

[0063] Put 10g of peptone, 10g of glucose, 5g of sodium chloride, and 5g of beef extract into a large beaker; add 1000ml of water, and adjust the pH to 6.8-7.5 after it is completely dissolved; divide it into a small triangle bottle (200ml), and plug the bottle Plug the kraft paper and tie it; the culture medium is sterilized and kept at 121°C for 15 minutes;

[0064] 2) Production of liquid strains

[0065] Uncover the plate cover with the cover clip, and clamp the plate with the clip supporting the plate; scrape off the aerial hyphae of JAUH-W-02 on the surface of the plate with an inoculation hook; use an inoculation knife to cut evenly at a distance of 2cm from the old inoculation block 3-5mm size JAUH-W-02 bacteria block; fix the plate in the clamp of the plate, put it on the tripod, burn the inoculation hook, put it on the...

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Abstract

The invention relates to a pure white black fungus strain and the application thereof and belongs to the technical field of edible mushrooms. The pure white black fungus strain JAUH-W-591, Auriculariaheimuer has a collection number of CGMCC No. 15374 in China General Microbiological Culture Collection Center, is collected on April 11, 2018, and has the collection address of 3#, Yard 1, Beichen West Road, in Chaoyang District of Beijing, in Institute of Microbiology, Chinese Academy of Sciences. The pure white black fungus strain disclosed by the invention has the advantages that the pure white black fungus strain JAUH-W-591 has the advantages of being white as polished jade in color, smooth, transparent and excellent in water-absorbing quality, has a high soaked rate of about 1 to 15, istender, smooth and sticky in texture after soaked, has a good mouth feel, and can be subjected to overwintering cultivation in the Northeast China Region. The commodity traits of auricularia fuscosuccinea such as texture, mouth feel and the like are effectively improved while the single original color of the black fungus is changed, so that the product has uniqueness and competitiveness in the market.

Description

technical field [0001] The invention relates to the technical field of edible fungi, in particular to a pure white black fungus strain JAUH-W-591 and its application. Background technique [0002] Black fungus (Latin name Auriculariaheimuer) belongs to Basidiomycota, Agaricomycetes, Auriculares, Auriculariaceae, and Auricularia. Also known as light fungus, black ear, cloud ear, black cabbage and so on. China is the first country in the world to cultivate fungus. The earliest record about the method of planting fungus is "boil the porridge and put it on the trees, and cover it with grass, that is, the fungus." mentioned. The artificial cultivation of black fungus originated in China around 600 AD, and is the first artificially cultivated edible fungus variety in the world. In 2017, the total output of black fungus in my country reached more than 6 million tons, making it the fourth largest cultivated edible fungus in the world. [0003] Black fungus is rich in nutrition a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G18/00C12Q1/6895C12N15/11A01H15/00A23L31/00A23L33/00
CPCA01H15/00A23L31/00A23L33/00A01G18/00C12Q1/6895C12N1/145C12R2001/645
Inventor 李晓
Owner 泌阳县玉润农业科技有限公司
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