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A method of inducing milk vetch tetraploid

A technology of vetch and tetraploid, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc.

Inactive Publication Date: 2020-04-10
南京理想农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of colchicine + trifluralin + Tween T-20 mixture as a mutagen

Method used

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  • A method of inducing milk vetch tetraploid
  • A method of inducing milk vetch tetraploid
  • A method of inducing milk vetch tetraploid

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Experimental program
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Embodiment 1

[0027] 1.1 Materials

[0028] The "Zidifeng" milk vetch variety is a diploid (2n=2x=16) milk vetch variety selected by Nanjing Ideal Agricultural Technology Co., Ltd.; colchicine and trifluralin are products of Shanghai Yuanye Biotechnology Co., Ltd. , colchicine purity 98%, white powder; trifluralin purity 97%, pink solid; Tween T-20 is a product of Guangzhou Zhongwan New Material Co., Ltd.

[0029] On September 19, 2016, the seeds were sown in plug trays (50 holes) equipped with seedling substrates (Huai'an Chaimihe Agricultural Science and Technology Development Co., Ltd.), and placed in an artificial climate chamber for cultivation. The optical density was controlled at 240 μmol m -2 ·s -1 , light hours 10h·d -1 , daytime temperature 25°C, night temperature 18°C, relative humidity 70% to 85%, regular ventilation [42] .

[0030] 1.2 Processing method

[0031] When the cotyledon petiole angle is 30°~50°, when the first true leaf is not out, the growth point is treated w...

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Abstract

The invention discloses a method for inducing an astragalus sinicus tetraploid. The method comprises the following step: by using a combined mutagenic agent, performing dropping treatment on a growingpoint when a cotyledon-petiole included angle of a diploid astragalus sinicus ranges from 30 degrees to 50 degrees and a first true leaf is not released, wherein after the treatment, the optical density is reduced to 180 [mu]mol.m<-2>.s<-1>, the illumination time is controlled to be 10 h.d<-1>, the day temperature is 18 DEG C, the night temperature is 15 DEG C, and the relative humidity is 75% to90%; the combined mutagenic agent comprises 0.1 to 0.2% of colchicine, 50 to 100 mg.L<-1> of trifluralin, and 20 [mu]l.L<-1> Tween T-20; the treatment using the combined mutagenic agent is performedfor 2 to 4 times. According to the method, colchicine and trifluralin at specific concentrations are used in a mixed manner, so that the tetraploid induction rate reaches 13.7% and is improved; in addition, the concentration of colchicine is reduced, so that environmental pollution is reduced.

Description

technical field [0001] The invention belongs to the field of molecular breeding and relates to a method for inducing tetraploid of milk vetch. Background technique [0002] Milk vetch is an annual or perennial herb of the genus Astragalus in the leguminous family. It is the main green manure crop in winter paddy fields in southern my country. After plowing and retting, it can reduce the use of chemical fertilizers, increase soil nutrients, increase rice yield, and improve rice quality. Using winter fallow fields to plant milk vetch green manure, and carrying out reasonable nutrient management, can maintain high rice yield while improving nitrogen use efficiency, which is of great significance for achieving high quality and high yield of rice, and is an effective measure for raising and fertilizing fields . The Fifth Plenary Session of the 18th CPC Central Committee proposed to explore the implementation of a pilot system for crop rotation and fallow, and formulated the "Pil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 周金泉周鸿章李艳艳杨阳司聪聪陈俊川曹寿根张蜀宁
Owner 南京理想农业科技有限公司
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