Preparation method of bionic phospholipid membrane with controllable shape

A technology of biomimetic phospholipids and phospholipids, which is applied in the direction of using substances to absorb and weigh, can solve the problems of consuming resources and increasing experimental procedures, and achieves the effects of saving resources, simple operation process, good stability and reproducibility

Inactive Publication Date: 2018-11-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This not only increases the experimental process, consumes more resources, but may also take more time

Method used

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  • Preparation method of bionic phospholipid membrane with controllable shape
  • Preparation method of bionic phospholipid membrane with controllable shape
  • Preparation method of bionic phospholipid membrane with controllable shape

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of a kind of biomimetic phospholipid membrane (phospholipid bilayer membrane) of the present embodiment, concrete preparation steps are as follows:

[0037] (1) DPPC (dipalmitoylphosphatidylcholine) and cholesterol were weighed and mixed at a molar ratio of 7:2, and dissolved in chloroform. The organic solvent was evaporated on a rotary evaporator to obtain a phospholipid film. Add Tris buffer solution without metal cations, and hydrate at 65°C for 1h. Sonicate for about 30 min to obtain a vesicle suspension. The resulting vesicle suspension was extruded 11 times through a polycarbonate track-etched membrane (pore size 100 nm) using a LiposoFast handheld liposome extruder to obtain a phospholipid vesicle solution.

[0038] (2) Use a hydration solution (Tris buffer solution without metal cations) to dilute the vesicle solution to a phospholipid concentration of 0.05 g / L. The diluted vesicle solution was flowed over the surface of a gold chip (a gold ch...

Embodiment 2

[0041]The preparation of a biomimetic phospholipid membrane (supported vesicle membrane with a vesicle particle size in the range of 100-200nm) of this embodiment, the specific preparation steps are as follows:

[0042] (1) DPPC (dipalmitoylphosphatidylcholine) and cholesterol were weighed and mixed in a molar ratio of 4:1, and dissolved in chloroform. The organic solvent was evaporated on a rotary evaporator to obtain a phospholipid film. Add 0.5mol / L NaCl in Tris buffer solution, and hydrate at 65°C for 1h. Sonicate for about 30 min to obtain a vesicle suspension. The resulting vesicle suspension was extruded 11 times through a polycarbonate track-etched membrane (pore size 100 nm) using a LiposoFast handheld liposome extruder to obtain a phospholipid vesicle solution.

[0043] (2) Dilute the vesicle solution to a phospholipid concentration of 0.1 g / L using a hydration solution (0.5 mol / L NaCl in Tris buffer solution). The diluted vesicle solution flowed over the surface ...

Embodiment 3

[0046] The preparation of a biomimetic phospholipid membrane (supported vesicle membrane with a vesicle particle size in the range of 200-800nm) in this embodiment, the specific preparation steps are as follows:

[0047] (1) DMPC (dimyristoylphosphatidylcholine), DPPC (dipalmitoylphosphatidylcholine) and cholesterol were weighed and mixed in a molar ratio of 10:1:3, and dissolved in chloroform. The organic solvent was evaporated on a rotary evaporator to obtain a phospholipid film. Add 5mmol / L MgCl 2 ·H 2 O aqueous solution, hydrated at 65°C for 1h. Sonicate for about 30 min to obtain a vesicle suspension. The resulting vesicle suspension was extruded 11 times through a polycarbonate track-etched membrane (pore size 100 nm) using a LiposoFast handheld liposome extruder to obtain a phospholipid vesicle solution.

[0048] (2) Use hydration solution (5mmol / L MgCl 2 ·H 2 O in water) to dilute the vesicle solution to a phospholipid concentration of 0.08 g / L. The diluted vesi...

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Abstract

The invention belongs to the technical field of preparation of phospholipid membranes and discloses a preparation method of a bionic phospholipid membrane with a controllable shape. The preparation method comprises the following steps: after mixing phospholipid and cholesterol, dissolving with an organic solvent; carrying out rotary evaporation to remove the organic solvent to obtain a phospholipid thin film; carrying out hydration and homogenization treatment on the obtained phospholipid thin membrane in sequence to obtain a vesicle solution with the grain diameter range of 50 to 150nm; afterdiluting the obtained vesicle solution by utilizing a hydration solution, enabling the vesicle solution to flow through the surface of a gold sheet, so as to enable phospholipid vesicles to be adsorbed on the surface of the gold sheet to reach saturation; then washing a vesicles adsorption layer on the surface of the gold sheet by utilizing the hydration solution, so as to obtain the bionic phospholipid membrane, wherein the hydration solution is a Tris buffering solution or a water solution which contains or does not contain metal cations. The phospholipid membranes with different shapes areprepared on the same surface through controlling types of the metal cations in the solution, and resources are saved; a preparation process has good stability and repeatability; an operation processis simple and the prepared bionic phospholipid membrane has a few of defects.

Description

technical field [0001] The invention belongs to the technical field of phospholipid membrane preparation, and in particular relates to a preparation method of a bionic phospholipid membrane with controllable morphology. Background technique [0002] Biofilm is an important material basis for cells to carry out life activities, and is closely related to cell energy conversion, protein synthesis, material transportation, information transmission, and cell movement. The biomimetic phospholipid film has a similar structure to the biological film, and the phospholipid film formed on the surface of the solid substrate is the most important surface analysis technique such as quartz crystal microbalance (QCM-D), atomic force microscopy (AFM), surface plasmon resonance (SPR) and surface plasmon resonance (SPR). The application of elliptically polarized light provides a platform, which provides the possibility and convenience for the study of the structure and function of biological m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N5/02
CPCG01N5/02
Inventor 邓春林洪佳丹
Owner SOUTH CHINA UNIV OF TECH
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