Method for Extraction and Detection of Vitamin A and E in Blood

Abstract

The invention discloses a method for extracting and detecting vitamins A and E in blood, comprising the following steps: S1. Collect a serum sample, add an extraction reagent to the serum sample, place it in a vortex oscillator for mixing, centrifuge with a high-speed centrifuge, absorb the upper liquid and place Inject the sample into the sample bottle for analysis; S2, select 5 serum samples of known concentrations of vitamin A and E as quality control products, add extraction reagents to the quality control products, and inject samples for analysis; S3, take the quality control products Utilize high-performance liquid chromatography to measure, and analyze the measurement results to form a standard curve; S4. The blood sample is also measured by high-performance liquid chromatography, and the measurement results are used to calculate the concentration of vitamin A and vitamin E in the serum sample to be tested according to the standard curve. The present invention extracts and injects samples directly with an organic solvent, the extraction solvent selected for the organic layer is not easy to volatilize, reduces the inaccurate concentration caused by solvent volatilization, can ensure the detection accuracy of vitamin A and E, shortens the detection time, and improves the detection efficiency .

Description

technical field [0001] The invention relates to the technical field of blood detection, in particular to a method for extracting and detecting vitamins A and E in blood. Background technique [0002] Vitamin A can promote growth and maintain the normal functions of epithelial tissues such as skin, mucous membrane, cornea, etc., and participate in the synthesis of rhodopsin; participate in multiple oxidation processes in the body, especially the oxidation of unsaturated fatty acids. Lipid metabolism is affected, which can increase the secretion of gonadotropic cells in the anterior pituitary gland, promote the production and activity of sperm, increase follicles, increase ovarian function, increase corpus luteum cells and enhance the effect of progesterone. Traditional detection of serum vitamin A and Method E, whether it uses n-hexane extraction or methanol acetonitrile to precipitate serum protein, blows the solvent dry with nitrogen blower, and then dissolves the sample wi...

AI Technical Summary

Problems solved by technology

[0002] Vitamin A can promote growth and maintain the normal functions of epithelial tissues such as skin, mucous membrane, cornea, etc., and participate in the synthesis of rhodopsin; participate in multiple oxidation processes in the body, especially the oxidation of unsaturated fatty acids. Lipid metabolism is affected, which can increase the secretion of gonadotropic cells in the anterior pituitary gland, promote the production and activity of sperm, increase follicles, increase ovarian function, increase corpus luteum cells and enhance the effect of progesterone. Traditional detection of serum vitamin A and Method E, whether using n-hexane extraction or methanol acetonitrile to precipitate the serum protein, dry the solvent with a nitrogen blower, and then use the mobile phase to dissolve the sample, the operation is cumbersome and not suitable for rapid clinical testing

Images