Preparation method of protein and sporopollenin-containing oral microcapsule

A technology of sporopollenin and microcapsules, which is applied in the field of preparation of protein oral microcapsules, which can solve the problems of inability to enter the intestinal tract for absorption, and achieve the effects of short cycle, good safety and complete form

Active Publication Date: 2018-11-20
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the harsh environment of the stomach, especially the influence of pepsin, most prot

Method used

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  • Preparation method of protein and sporopollenin-containing oral microcapsule
  • Preparation method of protein and sporopollenin-containing oral microcapsule
  • Preparation method of protein and sporopollenin-containing oral microcapsule

Examples

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Embodiment 1

[0028] 1. Preparation of sporopollenin

[0029] 1) Acid hydrolysis: weigh 2 g of Lycopodium spores, dissolve in 15 mL of 85% (w / v) phosphoric acid solution, stir slowly to form a uniform suspension, and then stir for 3 h, 5 h, 10 h, and 20 h at 70°C , After 30h, the spores after acid hydrolysis were collected by filtration.

[0030] 2) Washing: the acid hydrolysis-treated spores were washed 6 times with water, 2 times with acetone, 1 time with 2M hydrochloric acid, 6 times with water, 1 time with acetone, and 3 times with ethanol.

[0031] 3) Drying the washed spores to constant weight at 60°C.

[0032] Through the above treatment, the spore content is decomposed and dissolved, leaving only the sporopollenin outer wall of the cavity structure. The particle size and surface morphology of sporopollenin were observed by electron microscope scanning. The results showed that the particle size distribution of sporopollenin after acid hydrolysis for 10 h and 20 h was uniform, and t...

Embodiment 2

[0050] 1) Preparation of sporopollenin: dissolving lycopodium spores in 70% (w / v) phosphoric acid solution, stirring for 20 h to carry out acidolysis, after filtering, washing with water 6 times, acetone 4 times, 1M hydrochloric acid 2 times, ethanol Washing twice to remove the spore content, leaving only the sporopollenin with a cavity structure, and then drying the sporopollenin;

[0051]2) Drug loading: Dissolve sporopollenin and lactase in 15 times the weight of water together, the weight ratio of sporopollenin and lactase is 5:1, stir at room temperature and under vacuum conditions for embedding, so that lactase embedded within the cavity structure of the sporopollenin, then filtered and the solid dried;

[0052] 3) Encapsulation of the outer wall of the sporopollenin: dissolving the sporopollenin loaded with drugs in a carboxymethylpachysan solution with a weight concentration of 3%, blocking the cavity structure of the sporopollenin with a polymer polysaccharide, and th...

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Abstract

The invention discloses a preparation method of a protein-containing oral microcapsule. The preparation method comprises the following steps: 1), acidolyzing lycopodium spores, then washing by using water, an organic solvent and acid so as to remove spore contents and leave only sporopollenin adopting a cavity structure, and then drying the sporopollenin; 2), dissolving the sporopollenin and protein together in water for embedding so as to embed the protein into the cavity structure of the sporopollenin, then filtering and drying a solid; 3), dissolving the medicine-loaded sporopollenin in a high molecular polysaccharide solution, and blocking the cavity structure of the sporopollenin by using high molecular polysaccharide, then adding a crosslinking agent for crosslinking the high molecular polysaccharide, solidifying, finally filtering, and drying the solid. Degradation and release of the therapeutic protein in the stomach are reduced, so that the protein can be smoothly absorbed through the stomach; the prepared microcapsule has a small particle size, uniform distribution and complete morphology, and is a novel medicinal dosage form with a good therapeutic effect, good safety, stable nature and reliable quality.

Description

technical field [0001] The invention relates to a preparation method of protein oral microcapsules, in particular to a preparation method of protein oral microcapsules using lycopollenin as a capsule material. Background technique [0002] Microcapsules are a new type of preparation widely used in cosmetics, medicine and food industries, and can be used for oral or topical administration, controlled release or taste masking and other fields. Despite the extensive experience gained in recent decades through extensive research into drug microencapsulation, how to rapidly and inexpensively manufacture microcapsules of uniform size, porosity, and structure, especially for oral administration of therapeutic proteins However, there are still many problems in microcapsules. [0003] In recent years, therapeutic proteins have been widely used in the treatment of human and animal diseases, occupying an increasing share in the pharmaceutical market, but due to the irritation and pain...

Claims

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Application Information

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IPC IPC(8): A61K9/52A61K47/46A61K47/36A61K38/47
CPCA61K9/5036A61K9/5063A61K38/47C12Y302/01108
Inventor 李斌邓紫玙梁宏闪李晶李艳
Owner HUAZHONG AGRI UNIV
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