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Multicellular system for regulating products by changing ventilating condition and fermenting method

A fermentation method and multi-cell technology, applied in the field of bioengineering, to achieve the effects of strong tolerance to complex substrates, reduced raw material costs, and good fermentation stability

Pending Publication Date: 2018-11-27
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing microbial flora products are relatively stable and only target one target product

Method used

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  • Multicellular system for regulating products by changing ventilating condition and fermenting method
  • Multicellular system for regulating products by changing ventilating condition and fermenting method
  • Multicellular system for regulating products by changing ventilating condition and fermenting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Enrichment and screening of domesticated 1,3-propanediol-producing multicellular system DUT08 from petroleum wastewater

[0043] The sample was collected from a petroleum wastewater treatment plant in Dalian, and 10 mL was inserted into the seed medium, and cultivated at 37°C and 200 r / min until more than half of the substrate was consumed (about 30 hours) to obtain a primary culture. Transfer the primary culture to another seed medium at a 5% inoculum size, cultivate until more than half of the substrate is consumed (about 16.5 hours) to obtain a secondary culture, and continue to transfer the secondary culture at a 5% inoculum size, The transfer time was shortened to 12 hours, and then the 12-hour subculture cycle was continuously subcultured to the 35th generation, and a multi-cell system with stable fermentation performance was obtained. Depend on figure 1 It can be seen from A and 1B that when the multi-cell system is subcultured to the middle and late s...

Embodiment 2

[0047] Example 2 Multicellular system DUT08 microaerobic / anaerobic fermentation metabolic characteristics and substrate tolerance

[0048] After culturing the multicellular system DUT08 obtained in Example 1 with the seed medium, it was inoculated into a 5L fermenter with 2L fermentation medium at a volume ratio of 10% for cultivation, and batch fermentation was carried out at an initial glycerol concentration of 40-140g / L (crude glycerol 53-187g / L), the results are shown in figure 2 ( figure 2 A:0.00vvm, figure 2 B: 0.02vvm). Fermentation results showed that the multicellular system DUT08 was 2 Under the condition of feed rate of 0.00vvm, the highest tolerance is 120g / L glycerol, and the fermentation products are mainly 1,3-propanediol and lactic acid. When the initial glycerol concentration was 120g / L, the concentration of 1,3-propanediol reached 43.2g / L, the concentration of lactic acid was 26.38g / L, and the total mass conversion rate of 1,3-propanediol and lactic a...

Embodiment 3

[0049] Example 3 Multicellular system DUT08 in different N 2 Growth and Metabolism at Influx

[0050] After the multicellular system DUT08 obtained in Example 1 was cultivated in the seed medium, it was inoculated into a 5L fermentor with 2L fermentation medium by 10% volume ratio for cultivation, the initial substrate concentration was 120g / L, and the ventilation rate was 0-0.2vvm, aeration for 1h and 2h before and after inoculation, see fermentation results image 3 . The fermentation results showed that with the increase of the ventilation rate, the ability of the multi-cell system DUT08 to ferment and produce 1,3-propanediol gradually increased. When the ventilation rate was 0.1vvm, the highest yield of 1,3-propanediol was 61.80g / L. It is 0.55g / g, and the production intensity is 2.12g / (L·h). When the ventilation rate increased to 0.2vvm, the output of 1,3-propanediol was 61.49g / L, the conversion rate was 0.55g / g, and the production intensity increased to 2.46g / (L·h). Wi...

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Abstract

The invention discloses a multicellular system for regulating products by changing the ventilating condition and a fermenting method. The multicellular system comprises anaerobic clostridium strains,facultative klebsiella pneumonia strains and escherich's bacillus strains, the total number of effective living bacteria is 97 percent or above that in the multicellular system, wherein the ratio of effective living bacteria of the anaerobic clostridium strains, facultative klebsiella pneumonia strains and escherich's bacillus strains is (60-90):(5-40):(1-10). The multicellular system has stable fermenting performance and strong tolerance under micro-aerobic / anaerobic condition, and can be used for efficiently transforming crude glycerine into 1,3-propylene glycol, lactic acid and butyric acid, different multicellular factories can be constructed from cell sizes by flexible combination of different cells of microorganisms, and the material flow and energy flow of cellular metabolism of microorganisms can be re-distributed by manually controlled recombination and optimization, so that platform compounds can be efficiently prepared.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method that uses crude glycerol as a substrate and flexibly regulates the composition of a multicellular system by changing aeration conditions, thereby realizing the regulation and production of different products. Background technique [0002] Biorefinery is a new industrial model for producing energy and chemical products from bio-renewable resources, and is considered to be the most promising technology for creating new bio-based industries. Cell factories are the basis for biochemical transformations. However, any microorganism in nature has limited types of enzymes, limited conversion efficiency and capacity, and cannot meet the needs of industrial production. To transform microorganisms into cell factories for biorefinery, it is necessary to use the latest developments in genomics, systems biology, and high-speed computing technology to analyze the es...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12P7/52C12P7/18C12R1/145C12R1/22C12R1/19
CPCC12P7/18C12P7/52C12P7/56
Inventor 孙亚琴郑亚峰周瑾洁修志龙
Owner DALIAN UNIV OF TECH
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