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A kind of broad-spectrum botanical fungicide and its preparation method and application

A technology for fungicides and phytopathogenic fungi, which is applied in the fields of botanical equipment and methods, fungicides, plant growth regulators, etc., and can solve the problems of low bactericidal activity, narrow target, and no broad-spectrum bactericidal activity.

Active Publication Date: 2020-04-28
HAINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many plant extracts have been proven to have fungicidal activity against phytopathogenic fungi, such as thyme, garlic, cnidium, dandelion, knotweed, etc., but some of these known natural products have low fungicidal activity or target specific The target is relatively narrow, does not have broad-spectrum bactericidal activity, the separation procedure is complicated, and the cost is relatively high

Method used

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  • A kind of broad-spectrum botanical fungicide and its preparation method and application
  • A kind of broad-spectrum botanical fungicide and its preparation method and application
  • A kind of broad-spectrum botanical fungicide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Study on the Activity of the Crude Extract from the Stem of Shepodia

[0049] 1) Preparation

[0050]Weigh an appropriate weight of the stem of Shepod, dry it in a blast drying oven at 50°C, grind it into powder, extract it with methanol, ethyl acetate, acetone, dichloromethane and water, mix and soak each 100g powder with 1L solvent After soaking for 24 hours, the extract was collected and filtered with filter paper, and evaporated to dryness with a rotary evaporator at 50° C. to obtain five kinds of crude extracts of the stems of Snakewort with bactericidal activity.

[0051] 2) In vitro antifungal activity test

[0052] The bactericidal activity of the crude extracts of the above five phases was detected by the mycelium growth rate method.

[0053] A certain weight of the five extract phases was dissolved in DMF (0.1 mL), and then mixed with potato dextrose agar (PDA; 9.9 mL), and finally formulated to the required concentration. The crude extract was te...

Embodiment 2

[0075] Embodiment 2 has the separation of bactericidal activity compound and activity measurement

[0076] 1) Preparation

[0077] Get the dichloromethane crude extract of the stem of Serpentia chinensis prepared in Example 1, carry out initial separation with a silica gel column of 200-300 mesh silica gel, select sherwood oil / acetic acid solvent system for use, use eluents of different volume ratios respectively ( 100 / 0, 95 / 5, 90 / 10, 50 / 50, 0 / 100) the crude extract was separated to obtain 5 fractions, denoted as F1, F2, F3, F4, F5. Through activity screening, the F5 fraction showed the highest bactericidal activity.

[0078] Take F5, use Sephadex LH-20 gel column, use methanol as eluent isocratic elution, carry out further separation to F5, every 10mL is a cut, monitor by thin-layer chromatography TLC, finally combine and obtain 11 cuts, record Fr1, Fr2, Fr3, Fr4, Fr5, Fr6, Fr7, Fr8, Fr9, Fr10, Fr11. Among them, Fr4 and Fr7 showed the highest bactericidal activity.

[007...

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Abstract

The present invention provides a broad-spectrum plant source fungicide, and a preparation method thereof and an application thereof. The provided broad-spectrum plant source fungicide comprises: a waltheria indica stem extract. The waltheria indica stem extract, especially a dichloromethane crude extract and isolated and purified compounds of antidesmone and waltherione C, has advantages of beinghighly efficient, low in toxicity, low in residue, free of public hazard, low in cost, strong in sterilization activity, broad in antimicrobial spectrum, simple in separation processes, etc.; the sterilization activity and broad spectrum of the broad-spectrum plant source fungicide are much higher than those of commercial plant source fungicides of cnidium lactone, carvacrol and eugenol; and the broad-spectrum plant source fungicide does not produce cross-resistance with other fungicides, has a special effect on carbendazim-resistant sclerotinia sclerotiorum, has a certain negative cross-resistance relationship with carbendazim, and can be used as the potential fungicide for fungal diseases of farmland plants.

Description

technical field [0001] The invention relates to the technical field of fungicides, in particular to a broad-spectrum botanical fungicide and its preparation method and application. Background technique [0002] For decades, phytopathogenic fungi have caused serious damage to crop production. There are more than 8,000 known species of fungi that can cause plant diseases. my country is a large agricultural country. Diseases caused by plant pathogenic fungi are an important factor restricting my country's agricultural production. These diseases often occur on a large scale and cause huge losses to my country's agricultural production. Currently, chemically synthesized pesticides are mainly used in agriculture. Our country needs to produce and use more than 800,000 tons of chemical fungicides and pesticides every year. The use of these pesticides has greatly reduced the losses caused by diseases, but the large-scale use of chemical pesticides has also caused A series of proble...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/42A01N43/90A01P3/00C07D215/24C07D491/18
CPCA01N43/42A01N43/90C07D215/24C07D491/18
Inventor 孙然锋梁财朱新月丁晓帆陈碧伟宋文
Owner HAINAN UNIV