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Vitamin D releasing agent and its preparation method and application

A release agent and vitamin technology, which is applied in the field of vitamin D release agent and its preparation, can solve the problems of complex components of the release agent, cumbersome preparation process, and many operation steps

Active Publication Date: 2021-09-10
德康润生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The first object of the present invention is to provide a vitamin D release agent to solve the technical problems of the prior art that the composition of the release agent is relatively complex, there are many operating steps and the processing time is long
[0006] The second object of the present invention is to provide a method for preparing the above-mentioned vitamin D release agent, so as to solve the technical problems in the prior art that the composition of the release agent is relatively complex and the preparation process is cumbersome

Method used

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  • Vitamin D releasing agent and its preparation method and application
  • Vitamin D releasing agent and its preparation method and application

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preparation example Construction

[0061] The present invention also provides a preparation method of the above-mentioned vitamin D releasing agent, comprising the following steps:

[0062] Preparation of the first reagent: take each raw material in the formula amount and stir evenly;

[0063] Preparation of the second reagent: take each raw material in the formulated amount, dissolve it, obtain a stock solution, and freeze-dry the stock solution.

[0064] The preparation method of the vitamin D releasing agent provided by the present invention is simple to operate, and by adopting a freeze-drying process, it can effectively relieve dithiothreitol from being oxidized by air, and can also effectively obtain bovine serum albumin and sodium caseinate. Protection, thereby prolonging the shelf life of the second reagent and ensuring a good release effect.

[0065] In a preferred embodiment, the freeze-drying process comprises the following steps:

[0066] (a) Freeze the stock solution at -80°C for 2 hours;

[006...

Embodiment 1

[0081] This embodiment provides a vitamin D releasing agent, including a first reagent and a second reagent; the first reagent includes 1 part of Tween-80, 100 parts of methanol and the balance of phosphate buffer in 1000 parts by weight;

[0082] The second reagent is mainly prepared from the following raw materials in parts by weight: 0.0115 parts of dithiothreitol, 0.75 parts of bovine serum albumin and 0.15 parts of sodium caseinate.

Embodiment 2

[0084] This embodiment provides a vitamin D releasing agent, including a first reagent and a second reagent; the first reagent includes 2 parts of Tween-80, 200 parts of methanol and the balance of phosphate buffer in 1000 parts by weight;

[0085] The second reagent is mainly prepared from the following raw materials in parts by weight: 0.023 parts of dithiothreitol, 1.5 parts of bovine serum albumin and 0.75 parts of sodium caseinate.

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Abstract

The invention relates to the technical field of in vitro diagnostic immunoassay reagents, in particular to a vitamin D releasing agent and its preparation method and application. The vitamin D release agent includes a first reagent and a second reagent; the first reagent includes 1 to 5 parts of Tween-80, 100 to 300 parts of methanol and a balance of phosphate buffer saline in 1000 parts by weight; the second reagent It is mainly prepared from the following raw materials in parts by weight: 0.0115-0.0462 parts of dithiothreitol, 0.75-2.25 parts of bovine serum albumin and 0.15-1.2 parts of casein sodium. The composition of the reagent of the present invention is relatively simple, the processing time is short, the time cost brought by the long-time processing is avoided, the complexity of the detection process is reduced, and the operation steps are fewer at the same time, the probability of introducing systematic errors is reduced, and the result is accurate. The controllability is stronger, and it is more conducive to popularization and use in clinical applications.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic immunoassay reagents, in particular to a vitamin D releasing agent and its preparation method and application. Background technique [0002] Vitamin D plays a very important role in human bone metabolism. 25-Hydroxyvitamin D is the main form of vitamin D in the body. Studies have shown that the content of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 can characterize the level of vitamin D in the human body. Clinically, these two items are combined to indicate the content of total vitamin D. [0003] The quantitative detection methods for vitamin D are roughly divided into: high performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS) and immunoassay. Among them, the immunoassay has been adopted by a large number of in vitro diagnostic reagent manufacturers, including Diasorin, Roche, Abbott, etc. Products related to the project were launched. [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N33/531
CPCG01N33/53G01N33/531
Inventor 杨玮王昊董常慧陶然王慧张锦萍
Owner 德康润生物科技(北京)有限公司