3D printed Ti-PDA-BMP-2 bone defect repair tissue engineering scaffold and preparation method thereof

A tissue engineering scaffold, BMP-2 technology, applied in tissue regeneration, additive processing, medical science and other directions, can solve the problems of unguaranteed biosafety, high price, tumorigenicity, etc., to improve the carrying efficiency, improve The effect of hydrophilicity and biocompatibility

Inactive Publication Date: 2018-12-07
南京冬尚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biologically active factors have the possibility of immune rejection, pathogen transmission, allergic reaction, potential tumorigenicity, and teratogenicity, and biological safety cannot be guaranteed, and clinical-grade biological factors such as rhBMP-2 and TGF-β often need to be imported, and The price is expensive, and the purchase price per milligram reaches nearly a thousand dollars

Method used

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  • 3D printed Ti-PDA-BMP-2 bone defect repair tissue engineering scaffold and preparation method thereof
  • 3D printed Ti-PDA-BMP-2 bone defect repair tissue engineering scaffold and preparation method thereof
  • 3D printed Ti-PDA-BMP-2 bone defect repair tissue engineering scaffold and preparation method thereof

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Experimental program
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Embodiment 1

[0037] 1. Preparation of 3D printed Ti scaffolds

[0038] The Ti6Al4V powder is sintered by laser to form a shaped fiber bundle, and the 3D printed Ti scaffold is obtained by stacking and splicing the fiber bundle at 0-90° or 0-60-120°, such as Image 6 shown.

[0039] 2. Preparation of 3D printed Ti-PDA scaffold

[0040] After the stent structure is constructed by 3D printing, the surface is modified using PDA. The specific steps include:

[0041] 1) preparation concentration is the hydrochloric acid (HCL) solution of 0.5mol / L;

[0042] 2) Weigh 0.61g Tris and add it into 500ml water to dissolve, after stirring, add the prepared 0.5mol / L HCL solution drop by drop to adjust to pH=8.5, and prepare Tris-HCL solution for later use;

[0043] 3) Dopamine (DA) was dissolved in the above 150ml Tris-HCL solution, stirred and dissolved, and prepared as 2% DA-Tris-HCL solution;

[0044] 4) Add the 3D printed Ti bracket to the above solution, stir in the dark and unsealed state, 800-...

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Abstract

The invention discloses a 3D printed Ti-PDA-BMP-2 bone defect repair tissue engineering scaffold and a preparation method thereof. The 3D printed Ti-PDA-BMP-2 bone defect repair tissue engineering scaffold is prepared from Ti6Al4V powder through a 3D printing technology and has a controllable shape, a pore microstructure and controllable physicochemical properties. A biological factor BMP-2 with bone repair ability is bonded to the engineering scaffold through polydopamine on the fiber surfaces and is used for repair and treatment on bone defects. The engineering scaffold has the advantages ofsimple and reliable structure, controllable shape and microstructure, simple and efficient surface modification, reliable mechanical property, high biological activity and safety, convenient implantation, small trauma and low cost.

Description

technical field [0001] The invention belongs to the field of bone tissue repair and reconstruction, and relates to a 3D printed Ti-PDA-BMP-2 bone defect repair tissue engineering scaffold and a preparation method thereof. Background technique [0002] The repair and reconstruction of bone defects is one of the clinical problems in orthopedics. It is well known that autogenous bone is considered the gold standard for bone grafting, but its source is limited and may lead to complications such as necrosis of the supply site, postoperative chronic pain, allergic reaction, and infection. While allograft bone and xenograft bone are widely used because they come from a wide range of sources and do not require additional surgical operations, but there are also complications such as slow integration and remodeling, immune rejection, and disease transmission, especially reducing the osteoinductive and osteoconductive. [0003] At present, scaffold materials include bioactive calcium...

Claims

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Application Information

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IPC IPC(8): A61L27/34A61L27/06A61L27/50A61L27/54A61L27/56B33Y10/00B33Y70/00
CPCA61L27/06A61L27/34A61L27/50A61L27/54A61L27/56A61L2300/414A61L2300/606A61L2420/02A61L2430/02B33Y10/00B33Y70/00B33Y80/00C08L79/02
Inventor 姚庆强王黎明徐燕于一帆
Owner 南京冬尚生物科技有限公司
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