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Culture medium for clean production of riboflavin and method

A technology of clean production and culture medium, applied in the field of biopharmaceuticals, can solve the problems of losing market share, not optimizing process control, not innovating, etc.

Inactive Publication Date: 2019-03-01
NINGXIA QIYUAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the next few years or ten years, if the current process route is maintained without innovation and process control is not optimized, the existing market share will be lost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Seed preparation: Inoculate the activated seed solution into the sterilized seed medium for cultivation, and the seed inoculation amount is 2%.

[0023] The composition of the seed medium:

[0024] Glucose 1.0%, betaine 0.2%, yeast extract powder 0.035%, ammonium sulfate 0.25%, urea 0.8%, magnesium sulfate 0.015%, light calcium carbonate 0.6%, and the rest is water. Prepare according to the weight-volume ratio, pressurize at 118±2°C for 15 minutes, insert the activated seed solution, stir at 39°C, ventilation rate 1:0.4v / v / min, and cultivate for 18hr to obtain the seed solution.

[0025] 2. Fermentation: Transfer the above seed liquid into the fermentation medium for cultivation, and the inoculum amount is 5%.

[0026] Fermentation medium composition:

[0027] Glucose 2.0%, betaine 0.35%, yeast extract powder 0.08%, urea 1.2%, ammonium sulfate 0.25%, magnesium sulfate 0.015%, light calcium carbonate 0.6%, dipotassium hydrogen phosphate 0.15%, the rest is water, by ...

Embodiment 2

[0038] 1. Seed preparation: Inoculate the activated seed liquid into the sterilized seed medium for cultivation, and the seed inoculation amount is 5%.

[0039] The composition of the seed medium:

[0040] Glucose 1.2%, betaine 0.3%, yeast extract powder 0.045%, ammonium sulfate 0.25%, urea 0.8%, magnesium sulfate 0.015%, light calcium carbonate 0.6%, and the rest is water. Press for 15 minutes, insert the activated seed solution, stir at 39°C, ventilation rate 1:0.4v / v / min, and cultivate for 15hr to obtain the seed solution.

[0041] 2. Fermentation: Transfer the above seed liquid into the fermentation medium for cultivation, and the inoculum amount is 5%.

[0042] Fermentation medium composition:

[0043] Glucose 2.2%, betaine 0.45%, yeast extract powder 0.10%, urea 1.2%, ammonium sulfate 0.25%, magnesium sulfate 0.015%, light calcium carbonate 0.6%, dipotassium hydrogen phosphate 0.15%, the rest is water, by weight volume Ratio preparation, 118±2°C pressure hold for 15mi...

Embodiment 3

[0053] 1. Seed preparation: Inoculate the activated seed solution into the sterilized seed medium for cultivation, and the seed inoculation amount is 8%.

[0054] The composition of the seed medium:

[0055] Glucose 1.2%, betaine 0.35%, yeast extract powder 0.05%, ammonium sulfate 0.25%, urea 0.8%, magnesium sulfate 0.015%, light calcium carbonate 0.6%, and the rest is water. Press for 15 minutes, insert the activated seed solution, stir at 39°C, ventilation rate 1:0.4v / v / min, and cultivate for 20 hours to obtain the seed solution.

[0056] 2. Fermentation: Transfer the above seed liquid into the fermentation medium for cultivation, and the inoculum amount is 10%.

[0057] Fermentation medium composition:

[0058] Glucose 2.5%, betaine 0.50%, yeast extract powder 0.12%, urea 1.2%, ammonium sulfate 0.25%, magnesium sulfate 0.015%, light calcium carbonate 0.6%, dipotassium hydrogen phosphate 0.15%, the rest is water, by weight volume Ratio preparation, 118±2°C pressure hold f...

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Abstract

The invention relates to a culture medium for clean production of riboflavin and a method. Molasses is replaced with glycine betaine, the matching proportion of glucose to molasses is regulated and controlled in basic materials and a fermentation process supplementary material culture medium, residues are reduced, and clean production is achieved. Meanwhile, traditional organic nitrogen source corn steep liquor and yeast powder are replaced with a novel organic nitrogen source baker's yeast extractum, metabolism regulation and bioengineering optimization are carried out, therefore, the biomassis increased, the fermentation period is shortened, and continuous beneficiating is achieved. By means of the method, fast growth of thalluses can be promoted, the specific growth rate can be increased, the thalluses are prompted to synthesize riboflavin efficiently, the fermentation culture time is shortened, and is shortened by 10 h to 20 h compared with a conventional process, the fermentationlevel is improved, in-tank residues are reduced (amino nitrogen is reduced by 52% to 68%), and the light-previous degree of fermented mash is improved by 17% to 20%; the aim of clean production is achieved, the production comprehensive cost is reduced, and the fine regulation and control are achieved.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, in particular to a culture medium and method for clean production of riboflavin. Background technique [0002] Riboflavin is vitamin B2, a water-soluble B vitamin with the molecular formula C 17 h 20 N 4 o 6 , Appearance is yellow to orange-yellow fine needle-like crystals, odorous, slightly bitter; unstable, easy to decompose; riboflavin plays an important role in respiration and biological oxidation. Riboflavin is often used in medicine, food fortification, animal feed additive (2-8mg / kg), and a small amount is used as an active ingredient in nutritional or therapeutic cosmetics. [0003] At present, the industrial production methods of riboflavin include chemical semi-synthesis and microbial fermentation. The production of riboflavin by microbial fermentation has a history of 60 years. After 1974, with the successful breeding of riboflavin high-yielding bacteria and the continu...

Claims

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Application Information

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IPC IPC(8): C12P25/00C12N1/20C12R1/125
CPCC12N1/20C12P25/00
Inventor 崔莉盛雪李学兵张志
Owner NINGXIA QIYUAN PHARMA
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