Method for preparing 3-acetyl ginsenoside F1

Abstract

The invention discloses a method for preparing 3-acetyl ginsenoside F1. The method comprises the following steps: 1, taking ginseng flower buds, crushing the ginseng flower buds, performing hot refluxing extraction by using 95% ethanol, and concentrating the obtained solution to form a paste which is total extract; 2, dissolving the total extract in 25% ethanol to adjust the concentration to 0.2 gcrude drug / mL, filtering the obtained solution, adding 0.8 BV of the filtered solution into an LX-68 macro-porous adsorption resin column with a column bed having a diameter-to-length ratio of 1:9, removing impurities by using 3 BV of 40% ethanol, then performing elution with 9 BV of 75% ethanol, collecting 8-9 BV of 75% ethanol eluate, performing reduced pressure concentration until no alcohol smell appears, and lyophilizing the obtained concentrate to obtain a crude lyophilized powder; and 3, performing high-speed countercurrent separation with the solvent system being n-butanol / ethyl acetate / methanol / water / chloroform having a volume ratio of 1:18:1:18:0.5, collecting an elution fraction corresponding to the 3-acetyl ginsenoside F1 according to obtained chromatogram, and concentrating the elution fraction until the elution fraction is dry in order to obtain the 3-acetyl ginsenoside F1.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to a method for preparing ginsenoside F1 derivatives. The ginsenoside F1 derivatives include 3-acetyl ginsenoside F1, 6'-acetyl ginsenoside F1 and 6'-malonylformyl Ginsenoside F1. Background technique [0002] Previous studies have found that 3-acetyl ginsenoside F1, 6'-acetyl ginsenoside F1, and 6'-malonylformyl ginsenoside F1 have excellent tyrosinase inhibitory activity and can be used to prepare whitening cosmetics. From the chemical structure point of view, 3-acetyl ginsenoside F1, 6'-acetyl ginsenoside F1, and 6'-malonylformyl ginsenoside F1 are derivatives of ginsenoside F1, but ginsenoside F1 is a derivative of tyrosine The inhibitory activity of the enzyme is extremely weak, far less than that of its derivatives. [0003] At present, the separation and preparation of 3-acetyl ginsenoside F1, 6'-acetyl ginsenoside F1, and 6'-malonylformyl ginsenoside F1 must rely on re...

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Problems solved by technology

From the chemical structure point of view, 3-acetyl ginsenoside F1, 6'-acetyl ginsenoside F1, and 6'-malonylformyl ginsenoside F1 are derivatives of ginsenoside F1, but ginsenoside F1 is a derivative of tyrosine Enzyme inhibitory activity is extremely weak, far inferior to its derivatives

[0003] At present, the separation and preparation of 3-acetyl ginsenoside F1, 6'-acetyl ginsenoside F1, and 6'-malonylformyl ginsenoside F1 must rely on repeated silica gel column chromatography, however, repeated silica gel column chromatography dead adsorption Strong, large loss, not suitable for large-scale preparation at all

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