Method for enzymatic preparation of ammonium gluconate from potatoes

Abstract

The invention relates to a method for enzymatic preparation of ammonium gluconate from potatoes. The method comprises the following steps: with potatoes as a raw material, carrying out enzymatic liquefaction and saccharification at first to obtain a potato saccharification solution; then with the saccharification solution as a raw material and ammonia water as a neutralizing agent, carrying out enzymatic conversion to obtain a crude ammonium gluconate product; and then subjecting the crude ammonium gluconate product to concentration and drying so as to obtain pure ammonium gluconate. As a mainfood crop, the acre yield of the potatoes is much higher than the acre yield of corn, so the source of the raw material is guaranteed, and cost is low; the potatoes are directly applied to production, the processes of raw material treatment and starch extraction are reduced, preparation process is simple, and raw material processing cost is greatly reduced; the prepared crude ammonium gluconate product is used for soil treatment, so a good carbon source and ammonium nitrogen are provided, other nutrients in the potatoes are brought in, and the proliferation of soil microorganisms and the improvement of soil ecology are benefited; and the prepared pure ammonium gluconate can be used as a component for a chelating agent, a disinfectant, an emulsifier, a skin-care product and the like.

Description

technical field [0001] The invention relates to a method for enzymatically preparing ammonium gluconate using potatoes as raw materials, and belongs to the technical field of microbial enzymes. Background technique [0002] Ammonium gluconate is a white crystalline powder with a weak ammonia smell, with a relative molecular mass of 213.2, soluble in water, insoluble in ethanol, ether, benzene and other organic solvents, easily soluble in humid air, and changes color when exposed yellow. Ammonium gluconate can be used as an acidifier for fabric printing, an industrial chelating agent, a disinfectant component, an ingredient in skin care products and an emulsifier for food preparation such as cheese. Since the ammonium gluconate molecule has both a microbially degradable carbon source and an ammonium salt required for crop growth, it has potential application prospects in soil remediation and governance. At present, the reported ammonium gluconate production method has two k...

AI Technical Summary

Problems solved by technology

Preparation of ammonium gluconate by fermentation of Aspergillus niger has a long fermentation period, and the purity of the product obtained is low; in the process of chemical preparation of ammonium gluconate, although the palladium catalyst has high activity and good selectivity, the various products generated in the reaction process A variety of impurities, coke, etc. will poison the palladium catalyst, and the activity and selectivity will decrease until there is no catalytic activity. During the use of the palladium catalyst, the activity will also decrease due to the particle aggregation and lattice changes of the catalyst itself. Deactivated catalysts are reactivated

[0004] At present, the existing production methods all use glucose or starch hydrolyzate as raw material to produce ammonium gluconate, and the cost of raw material is high, which limits the application of ammonium gluconate in soil remediation and management.

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