Compound bismerthiazol paddy rice disease prevention and control composition
A compound equatazole, rice disease technology, applied in the directions of biocides, animal repellents, plant growth regulators, etc., can solve problems such as decreased control effect and the like
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[0015] 1. In vitro antibacterial activity experiment
[0016] (1) Test method
[0017] Adopt turbidity method, tested the inhibitory activity of each experimental drug to rice bacterial blight (Xanthomonasoryza epv.oryzae (Xoo)), concrete operation steps are as follows:
[0018] A. Add 1000mL sterilized distilled water into a 2000mL beaker, add 5.0g peptone,
[0019] Yeast powder 1.0g, glucose 10.0g, beef extract 3.0g, after stirring evenly, adjust the pH to neutral (7.2±0.2) with sodium hydroxide aqueous solution;
[0020] B. Clean and sterilize the test tubes and put them on the test tube rack. Use a pipette gun to pipette 4.0mL of the solution in step A into each test tube and then add a rubber stopper. Pack each 6 test tubes once. Use a sterilizing pot at 121 Sterilize at ℃ for 20 minutes before use;
[0021] C. Weigh 0.00375-0.0042g of the sample to be tested in a centrifuge tube, dissolve it with 150 μL DMSO, pipette 80 μL and 40 μL into sterilized numbered centrifuge...
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