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Nanoparticle enhanced type hydrogel as well as preparation method and application thereof

A nanoparticle-enhanced technology, which is applied in the field of nanoparticle-enhanced hydrogel and its preparation, can solve problems such as excessive degradation speed and insufficient mechanical strength, and achieve great promotion and use value, low cost, and expanded application range Effect

Inactive Publication Date: 2018-12-21
HUNAN GUOSHENG GRAPHITE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hydrogel prepared from the natural material sodium alginate has good biocompatibility, easy degradation, and low toxicity to cells and organisms. However, its mechanical strength is insufficient and the degradation speed is too fast, which is not conducive to its application in tissue engineering. application in

Method used

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  • Nanoparticle enhanced type hydrogel as well as preparation method and application thereof
  • Nanoparticle enhanced type hydrogel as well as preparation method and application thereof
  • Nanoparticle enhanced type hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Dissolve 120mg of sodium alginate powder in 6ml of deionized water, stir with a magnetic stirrer until it is completely dissolved, then suspend the P-S nanoparticles in the sodium alginate solution at 5% of the mass of sodium alginate; stir evenly, and remove it from the solution by ultrasonication for 30s The small air bubbles were added to the 24-well cell culture plate according to the volume of 2ml per well.

[0029] According to Ca 2+ Molar ratios to -COOH f=0.27, 0.36, 0.54 Weigh 9.4mg, 12.4mg, 18.6mg of graphene oxide powder respectively, suspend each in 0.5ml deionized water, and then add it into the sodium alginate solution in the orifice plate , Blow evenly with the tip of a pipette, and the solution is white turbid and milky at this time.

[0030] Weigh 23.2mg, 30.6mg, and 46.4mg of gluconolactone according to different f values, respectively dissolve them in 200ul deionized water, add them to the corresponding solutions containing sodium alginate and graphe...

Embodiment 2

[0032] Dissolve 120mg of sodium alginate powder in 6ml of deionized water to obtain a 2% sodium alginate solution, stir with a magnetic stirrer until completely dissolved; then suspend the P-S nanoparticles in the sodium alginate solution according to 5% of the mass of sodium alginate In the solution, stir evenly, sonicate for 30s to remove small air bubbles in the solution, and then add it into a 24-well cell culture plate according to the volume of 4ml per well.

[0033] According to Ca 2+ The molar ratio to -COOH f=0.54 Weigh 18.6mg of graphene oxide powder, suspend each in 0.5ml of deionized water, then add it to the sodium alginate solution in the orifice plate, and blow it evenly with the tip of a pipette. At this time, the solution is White cloudy milky.

[0034] Weigh 92.8 mg of gluconolactone, dissolve it in 200ul of deionized water, then add it into the solution containing sodium alginate and graphene oxide, and stir slightly with a pipette tip to make the GDL solut...

Embodiment 3

[0036] Dissolve 120mg of sodium alginate powder in 6ml of deionized water to obtain a 2% sodium alginate solution, stir with a magnetic stirrer until completely dissolved; then mix equal amounts of P-S and P-P nanoparticles according to 5% of the mass of sodium alginate Suspended in sodium alginate solution, stirred evenly, ultrasonicated for 30s to remove small air bubbles in the solution, and then added to a 24-well cell culture plate at a volume of 4ml per well.

[0037] According to Ca 2+ The molar ratio to -COOH f=0.54 Weigh 18.6mg of graphene oxide powder, suspend each in 0.5ml of deionized water, then add it to the sodium alginate solution in the orifice plate, and blow it evenly with the tip of a pipette. At this time, the solution is White cloudy milky.

[0038] Weigh 92.8 mg of gluconolactone, dissolve it in 200ul of deionized water, then add it into the solution containing sodium alginate and graphene oxide, and stir slightly with a pipette tip to make the GDL solu...

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Abstract

The invention relates to nanoparticle enhanced type hydrogel as well as a preparation method and application thereof. The nanoparticle enhanced type hydrogel is prepared by a preparation method comprising the following steps: S1, preparing sodium alginate modified PLGA (Poly(lactic-co-glycolic acid)) nanoparticles; S2: preparing a sodium alginate water solution; adding the PLGA nanoparticles obtained by S1; then adding a graphene oxide suspension solution into the mixed solution; uniformly stirring to obtain a mixed solution for later use; S3: preparing a gluconolactone water solution and adding the gluconolactone water solution into a mixed solution obtained by S2; slightly stirring and placing for some time, so as to obtain the nanoparticle enhanced type hydrogel. According to the nanoparticle enhanced type hydrogel, a nanoparticle surface group and calcium ions are specifically combined and an electrostatic action force between the nanoparticles is adopted, so that a new network isformed in a hydrogel network, and the mechanical property of the hydrogel is enhanced to the greatest extent. Materials of the hydrogel provided by the invention have good biocompatibility and no toxin on organisms; the preparation method is simple and low in cost and does not need large-size equipment; the preparation method has relatively great popularization and application value.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a nanoparticle-enhanced hydrogel and its preparation method and application. Background technique [0002] Sodium Alginate (SA for short) is a polysaccharide extracted from natural brown algae, approved by the US Food and Drug Administration (FDA) for use in the field of biomedical materials. As a non-toxic, non-irritating, natural polymer material with good biocompatibility, sodium alginate can hydrolyze a large number of -COO- ions in aqueous solution, exhibit polyanionic behavior, and have certain adhesion. It is often used as a drug carrier in clinical treatment. The presence of a large number of carboxyl groups enables it to quickly combine with divalent metal ions to form a gel. The hydrogel prepared from the natural material sodium alginate has good biocompatibility, easy degradation, and low toxicity to cells and organisms. However, its mechanical strength is insuffi...

Claims

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Application Information

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IPC IPC(8): C08L67/04C08L5/04C08K3/04C08J3/075A61L27/20A61L27/18A61L27/52A61L27/50A61K9/06A61K47/36A61K47/34
CPCC08J3/075A61K9/06A61K47/34A61K47/36A61L27/18A61L27/20A61L27/50A61L27/52C08J2305/04C08J2367/04C08J2405/04C08J2467/04C08K3/042C08L5/04C08L67/04
Inventor 林前锋
Owner HUNAN GUOSHENG GRAPHITE TECH CO LTD
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