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A new strain of Klebsiella pneumoniae and its isolation method and application

A technology for Klebsiella pneumoniae and pneumonia, which is applied in the field of new Klebsiella pneumoniae strains and their isolation, can solve the problems of large harmful effects, hazards of residents' diet irrigation, and extensive pollution of phenol-containing industrial wastewater, and achieves high effect of tolerance

Active Publication Date: 2022-02-22
XIAN LONGHUA ENVIRONMENTAL PROTECTION TECHCO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phenolic industrial wastewater pollutes a wide area and has great harmful effects, which will cause serious harm to residents' diet, fish farming and crop irrigation

Method used

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  • A new strain of Klebsiella pneumoniae and its isolation method and application
  • A new strain of Klebsiella pneumoniae and its isolation method and application
  • A new strain of Klebsiella pneumoniae and its isolation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation and Identification of the strain of the present invention

[0030] (1) Sampling: Directly select the paper sewage treatment plant aeration tank activated sludge, bring the active sludge to the laboratory, take the mud mixture of the active sludge to inject sterilization and phenol as a culture solution of the only carbon source. , Add a bacterial liquid in a cone bottle;

[0031] (2) Enrichment: Configure 1000ml MPYE medium: protein 胨 3g, yeast paste 3g, 1M MGCL 2 1.6ml, 1M CaCl 2 1.6ml, the margin is pure water; each 500ml MPYE medium is mounted to 500ml blue cover glass bottle, sealing the cover, put the YAMATO high pressure sterilization pan 121 ° C high pressure sterilization for 30 min; take out after being put into the ultra-clean net workbench After 30 min, the bacterium step 1 was inoculated with 10% inoculation to a 250 ml conical bottle containing 100 ml of MPYE culture solution, placed in 35 ° C, 200R / min shaker, and observed the observatio...

Embodiment 2

[0039] Example 2: New strain ZS-01 performance

[0040] (1) Strength of phenol degradation characteristics

[0041] Phenol content determination and standard curve were made of 4-aminonaminicin method.

[0042] Phenol degradation rate is calculated by the following formula:

[0043] Phenol degradation rate (%) = (a 0 -A s ) / A 0 × 100%

[0044] Among them, a 0 - The corresponding phenol content in the blank reference test

[0045] A s - Phenol content of sample liquid to be tested in the strain

[0046] (2) The optimal degradation conditions of phenol Zs-01 strain ZS-01

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Abstract

The invention discloses a new strain of Klebsiella pneumoniae and its isolation method and application. The new strain is isolated and screened from the activated sludge in the aeration tank of the papermaking sewage treatment plant, and is preserved in the China Common Microorganisms Collection Management Center, the deposit name is ZS‑01, the deposit number is 16041, and the deposit date is June 30, 2018. The characteristics and performance of the strain: (1) characteristics: the colonies formed on the phenol MedA solid medium are relatively short and thick bacilli with a size of 0.5~0.8×1~2 μm, arranged alone or in pairs, forming light yellow colonies with a texture Uniform and opaque; large off-white viscous colonies formed on MPYE solid medium; without flagella, with capsules, the strain belongs to Enterobacteriaceae, and it is a Gram-negative stubby bacillus. (2) Performance: It has certain phenol degradation ability; it has high tolerance to phenol. The advantage is that it can degrade higher concentration of phenol and provide a new bacterial source for effectively treating higher concentration of phenol-containing wastewater.

Description

Technical field [0001] The present invention belongs to the field of bioengineering, involving a new strain of Chratcheltra, and its separation method and application thereof. Background technique [0002] Phenol is in various industrial wastewater such as refining plants and petrochemical manufacturing. Phenol is also a coal gasification and liquefaction process, and the main organic components in the condensed oil flow. Pharmaceuticals, logs, paper mills, paper mills, plastic plants, etc., are also phenol. Unprocessed phenol industrial wastewater cannot be discharged to open waters, otherwise the water and food processing water will have an odor. The Environmental Protection Agency has developed an emission standard: the content of phenol in surface water is less than 1 billion. Phenolic industrial wastewater pollution is wide, harmful, and will cause serious harm to resident diet, fish farming and crop irrigation. The phenol concentration in the water body is more than 50 ppb ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/22
CPCC12N1/02C12N1/20C02F3/34C02F2101/345C12R2001/22C12N1/205
Inventor 张安龙赵呈馨王雪青王欣奇罗清房玉婷王哲毅陈朵
Owner XIAN LONGHUA ENVIRONMENTAL PROTECTION TECHCO LTD