Modified fibroin

Abstract

A modified fibroin includes a domain sequence indicated by formula 1: [(A)nmotif-REP]m. The domain sequence has an amino acid sequence having a reduced (A)nmotif content compared to naturally-derivedfibroin, said reduction being equivalent to the deletion of at least one (A)nmotif. [In formula 1, (A)nmotif indicates an amino acid sequence comprising 4-20 amino acid residues. The number of alanineresidues relative to the total number of amino acid residues in (A)nmotif is at least 83%. REP indicates an amino acid sequence comprising 10-200 amino acid residues. m indicates an integer between 8and 300. A plurality of (A)nmotif etc., may be the same amino acid sequence or different amino acid sequences.]

Description

Technical field [0001] The present invention relates to the modification of fibroin. More specifically, the present invention relates to (A) n The modified fibroin with reduced motif content. In addition, the present invention also relates to a nucleic acid encoding the modified fibroin, an expression vector containing the nucleic acid sequence, a host transformed with the expression vector, and a product made from the modified fibroin. Background technique [0002] Fibroin is one of fibrous proteins and contains up to 90% of glycine residues, alanine residues, and serine residues related to the formation of β-sheets (Non-Patent Document 1). As silk fibroin, proteins (silk fibroin, bee silk protein, and spider silk protein) constituting silk produced by insects and spiders are known. [0003] Silk protein has excellent mechanical properties, moisture absorption properties and deodorizing properties, and is a raw material widely used as a raw material for clothes. In addition, si...

AI Technical Summary

Problems solved by technology

However, these production systems have a slow production rate and are not suitable for mass production corresponding to commercial levels (Patent Documents 4 and 5)

As a production system capable of mass production, a lot of recombinant silk fibroin production by organisms such as yeast, mould, gram-negative bacteria, and gram-positive bacteria have also been reported and some results have been achieved, but it has not yet been achieved. Industrial mass production of recombinant silk fibroin excellent in elongation and tensile strength (Patent Document 5)

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