Skin injury repair material and preparation method thereof

A technology for repairing materials and skin damage, applied in the field of repair, can solve problems such as no reports of skin accessory organ regeneration, and achieve excellent repair effect and skin damage repair effect.

Inactive Publication Date: 2018-12-25
SICHUAN NEO LIFE STEM CELL BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there are no reports on the regeneration of skin appendages in the articles and patents on the use of tissue-engineered skin to repair full-thickness skin damage.

Method used

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  • Skin injury repair material and preparation method thereof
  • Skin injury repair material and preparation method thereof
  • Skin injury repair material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 Preparation of material of the present invention and repair thereof

[0037] 1. Preparation of restoration materials

[0038] S1. The adipose tissue obtained by liposuction from healthy volunteers was taken, washed with injection-grade normal saline, and then digested;

[0039] Specifically, the adipose tissue obtained by liposuction was washed with injection-grade saline for 3-5 times; the washed adipose tissue was mixed with digestive juice at a volume ratio of 1:1, and placed in a constant temperature shaker at 37°C at a speed of 150rpm for digestion. 2h. The digestive solution is 0.2% type II collagenase solution.

[0040] S2. The mixed solution after the digestion is completed is centrifuged to collect the precipitate to obtain the vascular matrix component SVF.

[0041] Specifically, the digested adipose tissue fragments in S1 were centrifuged at 900g for 5-10min, and the lower layer was removed to obtain the vascular matrix component SVF, which co...

Embodiment 2

[0062] Embodiment 2 Preparation of material of the present invention and repair thereof

[0063] 1. Preparation of restoration materials

[0064] S1. The adipose tissue obtained by liposuction from healthy volunteers was taken, washed with injection-grade normal saline, and then digested;

[0065] Specifically, the adipose tissue obtained by liposuction was washed with injection-grade saline for 3-5 times; the washed adipose tissue was mixed with digestive juice at a volume ratio of 1:1, and placed in a constant temperature shaker at 37°C at a speed of 150rpm for digestion. 2h. The digestive solution is 0.2% type I collagenase solution.

[0066] S2. The mixed solution after the digestion is completed is centrifuged to collect the precipitate to obtain the vascular matrix component SVF.

[0067] Specifically, the digested adipose tissue fragments in S1 were centrifuged at 900g for 5-10min, and the lower layer was removed to obtain the vascular matrix component SVF, which con...

experiment example 1

[0088] Experimental example 1 The repair experiment of material of the present invention

[0089] 1. Modeling of skin injury animal models

[0090] The animal model of skin injury was established by a two-step method. The first step was to create a rat diabetic model through high-fat feeding and streptozotocin STZ injection. The second step was to cut two diameters on the left and right sides of the back of the rat with surgical scissors. A full-thickness skin defect of 2 cm indicates successful modeling.

[0091] Specifically, 12-week-old clean-grade or SPF-grade SD rats were selected, with a body weight of about 200 g. After passing the quarantine, they were fed for one week to adapt to the environment, and then fed with high-fat feed for 3 weeks. Inject streptozotocin STZ at 30 mg / kg body weight from the tail vein of rats. One week after the injection, the rat tail vein blood collection can be detected with a blood glucose meter, and the blood sugar of the rats is higher t...

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Abstract

The invention discloses a skin injury repair material. A small intestinal mucosa lower membrane serves as a substrate and is compounded with adipose mesenchymal stem cells to form the repair material.The repair material is good in repair effect and clinical application prospect.

Description

technical field [0001] The invention relates to the repair field, in particular to a repair material for skin damage and a preparation method thereof. Background technique [0002] The skin is the largest organ of the human body, a natural barrier between the human body and the outside world, and has the ability to protect various tissues and organs in the body from physical, chemical, and microbial invasion. In addition, the hair follicles, sweat glands, sebaceous glands and other accessory organs growing on the skin perform functions such as beauty, perspiration, and oil secretion and lubrication. The skin can heal itself after a certain amount of damage, but clinically common large-area burns, wounds, diabetic foot ulcers, etc. are often difficult to heal after a long period of time, or the skin heals in the form of connective tissue hyperplasia to form scars, which affects the appearance and normal function of the skin. Clinically, autologous or allogeneic skin transpla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/60
CPCA61L27/3629A61L27/3687A61L27/3834A61L27/60A61L2300/412A61L2430/40
Inventor 范兆心解慧琪汪祝乐陈强龙能吉黄益洲
Owner SICHUAN NEO LIFE STEM CELL BIOTECH
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