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Method for extracting spectinomycin from fermentation culture

A spectinomycin, fermentation and cultivation technology, applied in the direction of organic chemistry, can solve the problems of long extraction period, low yield, a large amount of acid-base wastewater, etc.

Active Publication Date: 2018-12-25
SHANDONG LUKANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the traditional extraction method of spectinomycin is usually resin adsorption analysis method, the yield is low, and the extraction cycle is long, resulting in a large amount of acid-base wastewater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Take 5L of spectinomycin fermentation liquid (the content of spectinomycin is 3780u / mL), adjust the pH value to 3.5 with hydrochloric acid with a concentration of 2mol / L, separate the solid from the liquid, and collect 4.8L of the filtrate; Sodium oxide solution to adjust the pH value to 6.5, add 1 L of ethyl acetate solution of propylnaphthalenesulfonic acid and ethyl acetate at a volume ratio of 1:4, and carry out ionization under stirring conditions. Exchange for 30min, stand for stratification, collect the organic phase containing spectinomycin; add 200mL of ammonium chloride solution with a concentration of 2wt.%, carry out water extraction for 30min under stirring conditions, stand for stratification, collect the organic phase containing spectinomycin Water phase 450mL; add activated carbon for decolorization, filter, the obtained decolorized solution is vacuum concentrated at 70°C, and when crystals are precipitated, perform cooling treatment (uniformly cool down ...

Embodiment 2

[0052] Get 100L of spectinomycin fermented liquid (the content of spectinomycin is 3960u / mL), adjust the pH value to 4.0 with sulfuric acid with a concentration of 2mol / L, separate the solid and liquid, and collect 97L of the filtrate; the filtrate is oxidized with 10wt.% hydrogen Potassium solution to adjust the pH value to 7.0, add nonylnaphthalenesulfonic acid n-butanol solution (prepared from nonylnaphthalenesulfonic acid and ethyl acetate at a volume ratio of 1:4) 20L, and carry out ion exchange under stirring conditions 30min, standing for stratification, collecting the organic phase containing spectinomycin; adding 4L of hydrochloric acid solution with a concentration of 2wt.%, carrying out water extraction for 30min under stirring conditions, standing for stratification, collecting 9L of aqueous phase containing spectinomycin Add active carbon for decolorization, filter, and vacuum-concentrate the obtained decolorized solution at 75° C., and carry out cooling treatment ...

Embodiment 3

[0054] Get 500L of spectinomycin fermented liquid (the content of spectinomycin is 4060u / mL), adjust the pH value to 3.0 with sulfuric acid with a concentration of 2mol / L, separate the solid and liquid, and collect 480L of the filtrate; the filtrate is 10wt.% ammonia solution Adjust the pH value to 5.0, add nonylnaphthalenesulfonic acid butyl acetate solution (prepared from nonylnaphthalenesulfonic acid and ethyl acetate at a volume ratio of 1:4) 96L, carry out ion exchange under stirring conditions for 30min, Stand for stratification, collect the organic phase containing spectinomycin; add 20L of sodium chloride solution with a concentration of 2wt.%, carry out water extraction for 30min under stirring conditions, stand for stratification, and collect 46L of aqueous phase containing spectinomycin Add active carbon for decolorization, filter, and vacuum-concentrate the obtained decolorized solution at 65°C, and carry out cooling treatment (uniformly cooling to 5°C after 4h) whe...

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Abstract

The invention provides a method for extracting spectinomycin from fermentation culture. A liquid ion exchanger is adopted to extract spectinomycin, sulfonic group in the liquid ion exchanger has highcombining ability with spectinomycin, so that spectinomycin can be selectively extracted quickly and accurately from a system containing a lot of impurities. By adopting the method, spectinomycin yield can be up to higher than 65%, purity can reach 98%, extracted spectinomycin is high in quality and meets EP and USP quality standards, and the method has the advantages of short period, little sewage discharge and low production cost.

Description

technical field [0001] The invention relates to the technical field of spectinomycin extraction, in particular to a method for extracting spectinomycin from fermentation culture. Background technique [0002] Spectinomycin is an aminoglycoside antibiotic produced by Streptomyces spectabilis through fermentation and extraction. It has antibacterial activity against most Gram-positive bacteria and Gram-negative bacteria. The popular isolates of Neisseria gonorrhoeae are highly effective, and the penicillin- and tetracycline-resistant gonorrhoeae are also highly sensitive to it. In the clinical treatment of infections caused by Neisseria gonorrhoeae, it has outstanding curative effect, less side effects and is widely used Safety and other characteristics have become one of the first-selected drugs for the treatment of gonorrhea in my country. In addition, the low toxicity and broad-spectrum antibacterial properties of spectinomycin make it more and more widely used in fields suc...

Claims

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Application Information

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IPC IPC(8): C07D493/04
CPCC07D493/04
Inventor 朱维忠陈东刘江来王宏轩诗峰赵宏强汪晨
Owner SHANDONG LUKANG PHARMA
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