Graphene-doped enzyme biosensor and preparation thereof and application in detecting phenol
A biosensor, graphene technology, applied in the field of electrochemical biosensors, can solve the problems of damage and high cost, and achieve the effect of low detection limit, wide detection range, increase of fixed amount and stability
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Embodiment 1
[0045] A preparation method based on a graphene-doped phenolase biosensor, comprising the following steps:
[0046] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder was polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, and then the glassy carbon electrode was placed in 10 mL of potassium ferricyanide solution (5 mmol / L K 3 Fe(CN) 6 +0.2mol / L KCl) at 0-0.8V, use cyclic voltammetry to scan 6 circles for electrode activation, take it out, wash it with distilled water, and dry it at room temperature to obtain a pretreated glassy carbon electrode;
[0047] (2) 100 mg of graphene oxide was ultrasonically stripped in 50 mL of deionized water for 4 h (ultrasonic power was 100 W), and 0.5 mL of aniline was added, followed by ultrasonication for 30 min, and 5 mL of 1.25 g of ammonium persulfate (APS) wa...
Embodiment 2
[0053] A preparation method of a graphene-doped phenolase biosensor, comprising the following steps:
[0054] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder was polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, and then the glassy carbon electrode was placed in 10 mL of potassium ferricyanide solution (5 mmol / L K 3 Fe(CN) 6 +0.2mol / L KCl) at 0-0.8V, use cyclic voltammetry to scan 6 circles for electrode activation, take it out, wash it with distilled water, and dry it at room temperature to obtain a pretreated glassy carbon electrode;
[0055] (2) After 100 mg of graphene oxide was ultrasonically stripped in 50 mL of deionized water for 4 h (ultrasonic power is 100 W), 0.5 mL of aniline was added and ultrasonication was continued for 30 min, and 5 mL of 1.25 g of ammonium persulfate ( APS) ...
Embodiment 3
[0064] A preparation method of a graphene-doped phenolase biosensor, comprising the following steps:
[0065] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder was polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, and then the glassy carbon electrode was placed in 10 mL of potassium ferricyanide solution (5 mmol / L K 3 Fe(CN) 6 +0.2mol / L KCl) at 0-0.8V, use cyclic voltammetry to scan 6 circles for electrode activation, take it out, wash it with distilled water, and dry it at room temperature to obtain a pretreated glassy carbon electrode;
[0066] (2) After 100 mg of graphene oxide was ultrasonically stripped in 50 mL of deionized water for 4 h (ultrasonic power is 100 W), 0.5 mL of aniline was added and ultrasonication was continued for 30 min, and 5 mL of 1.25 g of ammonium persulfate ( APS) ...
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