Method for rapidly detecting heavy metal ions in water-milled glutinous rice flour based on microsphere crystal cluster fluorescent system

Abstract

The invention belongs to the technical field of heavy metal detection, and particularly relates to a method for rapidly detecting heavy metal ions in water-milled glutinous rice flour based on a microsphere crystal cluster fluorescent system, which comprises the following steps: synthesizing up-conversion fluorescent nanoparticles, carrying out ultra-grain assembly on the up-conversion fluorescent nanoparticles to form microsphere crystal clusters, then modifying amino groups on the exteriors of the microsphere crystal clusters, and carrying out fluorescence detection to obtain the heavy metal ions in the water-milled glutinous rice flour. And adding an aptamer corresponding to the heavy metal, connecting the aptamer to the microsphere crystal cluster, and then putting into a polydopamine solution to obtain the detection system. Adding heavy metal standard solutions with different concentrations into the system, then detecting fluorescence intensity signal characteristic values of the solutions, and drawing a standard curve about the relationship between the heavy metal concentration and the fluorescence intensity according to the change of the fluorescence intensity. And the heavy metal concentration in an actual sample with unknown heavy metal content can be calculated by using the standard curve. The invention provides a novel heavy metal detection method, which realizes rapid and sensitive detection of heavy metals in water-milled glutinous rice flour.

Description

technical field [0001] The invention belongs to the technical field of heavy metal detection, in particular to a method for rapidly detecting heavy metal ions in water-milled glutinous rice flour based on a microsphere crystal cluster fluorescence system. Background technique [0002] Heavy metal pollution exists widely in food. Due to the existence of excessive heavy metal ions in some foods, oral intake of these foods will lead to chronic heavy metal poisoning. Rapid and accurate detection of heavy metals in environmental samples plays a very important role in timely detection and prevention of their proliferation. The existing commonly used methods for detecting heavy metals include atomic fluorescence spectrometry, cold vapor atomic absorption spectrometry, inductively coupled plasma mass spectrometry, potassium permanganate-potassium persulfate digestion method, and dithizone spectrophotometry. However, these methods all require professional testing personnel with prof...

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Problems solved by technology

However, these methods require professional inspectors to have professional operation skills, and dangerous chemicals such as sulfuric acid and potassium borohydride need to be used when processing samples, which will also pose a certain threat to the health of inspectors.

Moreover, the steps of these detection methods are cumbersome, time-consuming, require the use of professional instruments, expensive equipment, high maintenance costs, and high detection costs

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