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A preparation method and application of polypeptide-mediated biomimetic silica nanoparticles

A technology of silica and nanoparticles, which is applied in the field of inorganic materials, can solve the problems that functional templates cannot be used repeatedly, the preparation process is complicated, and the cost of implementation is increased, achieving important industrial application value, simple equipment, and convenient operation.

Active Publication Date: 2021-06-29
HUAQIAO UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the functional template used cannot be used repeatedly, so the preparation process of the silica nanoparticles is relatively complicated, which increases the cost of its realization.

Method used

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  • A preparation method and application of polypeptide-mediated biomimetic silica nanoparticles
  • A preparation method and application of polypeptide-mediated biomimetic silica nanoparticles
  • A preparation method and application of polypeptide-mediated biomimetic silica nanoparticles

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Embodiment Construction

[0036] Embodiments of the present invention will be described below with reference to the drawings. It should be noted that representation and description of components and processes that are not related to the present invention and known to those of ordinary skill in the art are omitted from the drawings and descriptions for the purpose of clarity.

[0037] The invention provides a rapid preparation method of polypeptide-mediated biomimetic silica nanoparticles, which comprises:

[0038] Step 1: Prepare high-purity elastin-like polypeptide ELP40 by ITC purification method; as a specific example, the process specifically includes:

[0039] Preparations: Clean up the ultra-clean workbench, sterilize with ultraviolet light for 20 minutes; add ampicillin sodium at a ratio of 1:1000 (ampicillin sodium:medium, v / v), such as adding 10 μL to 10 mL of medium; glycerol bacteria at a ratio of 1:100 ( The inoculation amount of strain: medium (v / v) ratio was added to LB liquid medium, su...

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Abstract

A preparation method and application of polypeptide-mediated biomimetic silica nanoparticles, by adopting an improved scheme (increasing the renaturation treatment of precipitation) to achieve rapid acquisition of elastin-like polypeptide ELP40; for elastin-like polypeptide ELP40-mediated In the precipitation of silica particles, the silicon source solution is improved so that it is ultrasonically mixed with the discrete graphite solution to achieve sufficient mixing of the particles, and then bionic silica nanoparticles with a regular spherical structure (and more concentrated particle size distribution) can be obtained. , the particle diameter is between 70nm and 400nm), the reaction conditions are mild, the equipment is simple, the operation is convenient, and it is easy to reach an industrial scale. It has important industrial application value and huge application potential. In industry, it can be applied to the field of immobilized enzymes; in biomedicine, it can be used as a good drug carrier, especially slow-release drugs.

Description

technical field [0001] The invention relates to the artificial synthesis of inorganic materials with specific nanostructures by means of biological methods, in particular to the preparation of biomimetic silica nanoparticles, especially the preparation method based on polypeptide-mediated biomimetic silica nanoparticles. Background technique [0002] There is a very common phenomenon in nature - biomineralization. In the phenomenon of biomineralization, silica accounts for a large part, which widely exists in sponges, bacteria, unicellular algae, higher plants and protozoa, etc. After in-depth research, scientists have found that, compared with the silica produced by artificial synthesis technology, these biological silicas have specific functions and complex structures that are difficult for the former to achieve. In addition, this biomineralization process is a very typical green and low-energy synthesis technology, because it is formed under relatively mild conditions an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C07K1/30C07K1/14C01B33/18B82Y30/00
CPCB82Y30/00C01B33/18C07K14/78
Inventor 张光亚林源清邱岳葛慧华
Owner HUAQIAO UNIVERSITY
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