Tyrosine-derived amygdalin-loaded hydrogel preparation method and application

A technology of amygdalin and hydrogel, which is applied to medical preparations containing no active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc., which can solve traumatic brain injuries that cannot be directly affected and are difficult to achieve curative effect and other problems, to achieve good anti-inflammatory effect, low cost and easy injection

Active Publication Date: 2019-01-04
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Laetrile has a good anti-inflammatory pharmacological effect, but because the traditional Chinese medicine has a certain half-life in the human body, and can not directly act on the site of traumatic brain injury, it is difficult to achieve the corresponding curative effect, so a kind of New ways to enhance its healing properties

Method used

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  • Tyrosine-derived amygdalin-loaded hydrogel preparation method and application
  • Tyrosine-derived amygdalin-loaded hydrogel preparation method and application
  • Tyrosine-derived amygdalin-loaded hydrogel preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Weigh 5.0 mg / mL of Fmoc-Tyr-OH solid powder, dissolve it in 100 μL of dimethyl sulfoxide, and ultrasonically disperse it for 30 seconds to completely dissolve it to prepare a Fmoc-Tyr-OH stock solution with a concentration of 50 mg / mL; weigh Take 5.0 mg of amygdalin in a centrifuge tube, add 1.0 mL of ultrapure water to dissolve it, and prepare a stock solution of amygdalin with a concentration of 5 mg / mL.

[0038] Take 50μL of Fmoc-Tyr-OH stock solution and add it to a washed and dried 5.0mL screw bottle, add 970μL of amygdalin stock solution, shake it up quickly, and let it stand for 3 minutes to form a clear and transparent tyrosine derivative Hydrogel loaded with amygdalin.

[0039] SEM experimental steps:

[0040]Silicon wafers were ultrasonically cleaned with piranha solution (concentrated sulfuric acid (v):hydrogen peroxide (v)=7:3) for 15 minutes, then ultrasonically cleaned with ethanol solution for 15 minutes, and finally cleaned with ultrapure water for 15 m...

Embodiment 2

[0043] Weigh 5.0 mg / mL of Fmoc-Tyr-OH solid powder, dissolve it in 100 μL of dimethyl sulfoxide, and ultrasonically disperse it for 30 seconds to completely dissolve it, and prepare a Fmoc-Tyr-OH stock solution with a concentration of 100 mg / mL; weigh Take 16.0 mg of amygdalin in a centrifuge tube, add 1.0 mL of ultrapure water to dissolve it, and prepare a stock solution of amygdalin with a concentration of 16 mg / mL.

[0044] Take 30μL of Fmoc-Tyr-OH stock solution and add it to a washed and dried 5.0mL screw bottle, add 950μL of amygdalin stock solution, shake it up quickly, and let it stand for 3 minutes to form a clear and transparent tyrosine-loaded amygdalin Hydrogel of amygdalin.

[0045] Fourier transform infrared test experimental procedure: After drying the sample in a freeze dryer for 12 hours, weigh 0.2g of potassium bromide powder and then add 2mg of the sample, put it into a mortar and grind it, mix the two thoroughly, and then put The mixture is put into a mold...

Embodiment 3

[0047] Weigh 5.0 mg / mL Fmoc-Tyr-OH solid powder, dissolve it in 100 μL dimethyl sulfoxide, and disperse it ultrasonically for 60 seconds to completely dissolve it to prepare a Fmoc-Tyr-OH stock solution with a concentration of 75 mg / mL; weigh Take 1.0 mg of amygdalin in a centrifuge tube, add 1.0 mL of ultrapure water to dissolve it, and prepare a stock solution of amygdalin with a concentration of 1 mg / mL.

[0048] Take 40μL of Fmoc-Tyr-OH stock solution and add it to a cleaned and dried 5.0mL screw bottle, add 960μL of amygdalin stock solution, shake it up quickly, and let it stand for 3 minutes to form a clear and transparent tyrosine-loaded amygdalin Amygdalin hydrogel.

[0049] Rheological test experimental procedure: use AR2000 rheological instrument to test its rheological behavior, use a parallel plate with a diameter of 50mm, and set the gap to 0.2mm, test conditions: temperature is 25°C, intensity is 2%, frequency is 0.1— 100Hz.

[0050] Test results such as Figu...

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Abstract

The invention discloses a tyrosine-derived amygdalin-loaded hydrogel preparation method and application. The method includes: dissolving Fmoc-Tyr-OH solid powder into dimethyl sulfoxide, and ultrasonically dispersing to obtain Fmoc-Tyr-OH stock solution; dissolving amygdalin solution with ultrapure water to obtain amygdalin stock solution; well mixing Fmoc-Tyr-OH stock solution and amygdalin stocksolution, and standing to obtain tyrosine-derived amygdalin-loaded hydrogel. The tyrosine-derived amygdalin-loaded hydrogel has anti-inflammatory characteristics, the problem of failure in direct application of amygdalin monomer to traumatic brain injured parts can be solved, bioactivity of amygdalin is kept, and evident treatment effects are achieved in application to treatment of inflammatory reaction caused by traumatic brain injuries.

Description

technical field [0001] The invention relates to a preparation method and application of a tyrosine-derived hydrogel loaded with amygdalin, and belongs to the technical field of supramolecular hydrogels. Background technique [0002] Amygdalin, also known as vitamin B17, is a cyanide with the chemical formula C 20 h 27 NO 11 , the molecular weight is 457.42. Laetrile widely exists in many plants, especially in the seeds of Rosaceae plants, such as almonds, peach kernels, cherries, plums, etc. Amygdalin can be hydrolyzed and generate annattonitrile and mandelonitrile, and finally decompose into benzaldehyde and hydrocyanic acid under the action of glucosidase. Many studies have shown that amygdalin has cough and asthma, anti-atherosclerosis, inhibition of renal interstitial fibrosis, inhibition of pulmonary fibrosis, anti-hyperbaric oxygen-induced lung injury, immunosuppression, immune regulation, anti-tumor, anti-inflammatory and anti-ulcer role. Laetrile is used to tre...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K31/7028A61K47/18A61P25/00A61P29/00
CPCA61K9/06A61K31/7028A61K47/183A61P25/00A61P29/00
Inventor 王杨杨曌宇唐涛范荣罗杰坤崔寒尽
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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