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Rear spectrophotometric pupil laser differential confocal mass-spectrum microscopic imaging method and device

A differential confocal and microscopic imaging technology, applied in the field of confocal microscopic imaging technology and mass spectrometry imaging, can solve the problems of large laser focusing spot, low spatial resolution of mass spectrometry detection, long mass spectrometry imaging time, etc., to expand the application field , to achieve versatility, improve the effect of spatial resolution

Inactive Publication Date: 2019-01-11
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1) Due to the use of simple laser focusing to desorb and ionize the sample, there are still problems such as large laser focusing spot and low spatial resolution of mass spectrometry detection;
[0006] 2) The time required for mass spectrometry imaging is long, and the axial position of the focused spot of the laser mass spectrometer often drifts relative to the sample to be measured

Method used

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  • Rear spectrophotometric pupil laser differential confocal mass-spectrum microscopic imaging method and device
  • Rear spectrophotometric pupil laser differential confocal mass-spectrum microscopic imaging method and device
  • Rear spectrophotometric pupil laser differential confocal mass-spectrum microscopic imaging method and device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Such as figure 1 As shown, the collection pupil 11 is placed on the pupil plane of the detection objective lens 13 . The light source system 1 selects a point light source, and the excitation beam emitted by the point light source passes through the collimator lens 2, the compressed focusing spot system 4, the beam splitter 5, the dichroic mirror A6 and the measuring objective lens 7, and converges on the measured sample 8, and the computer 34 Control the precise three-dimensional workbench 10 to drive the measured sample 8 to move up and down near the focus of the measuring objective lens 7, and the light reflected by the sample is reflected by the dichroic mirror A6, reflected by the dichroic prism 5 and passed through the D-type collector in the D-type rear pupil 11 The pupil 12, the detection objective lens 13, and the relay magnifying lens 14 converge on the two-quadrant detector 16, and the first detection quadrant 17 and the second detection quadrant 18, which ar...

Embodiment 2

[0050] Such as figure 2 As shown, in the rear split-pupil laser differential confocal mass spectrometry microscopic imaging device, the compressed focus spot system 4 is replaced by a vector beam generation system 35 and a pupil filter 36, and the D-type rear pupil 11 can be replaced by a circular rear The pupil 37 is replaced, and the two-quadrant detector is replaced by a CCD detector 39, wherein the first micro-area of ​​the Airy disc and the second micro-area of ​​the Airy disc detected by the CCD detector are symmetrical about the optical axis.

[0051] The remaining imaging methods and processes are the same as in Example 1.

[0052] Example, 3

[0053] Such as image 3 As shown, in the post-divided pupil laser differential confocal mass spectrometer imaging device, the point light source 1 can be replaced by a pulse laser 40, a condenser lens 41, and a light-transmitting optical fiber 42 at the focal point of the condenser lens 41, and the light beam emitted by the l...

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Abstract

The invention discloses a rear spectrophotometric pupil laser differential confocal mass-spectrum microscopic imaging method and device, and belongs to the technical field of confocal microscopic imaging and mass-spectrum imaging measurement technologies. According to the method and the device in the invention, a rear spectrophotometric pupil laser differential confocal microscopic imaging technology and a mass-spectrum detection technology are combined; mass-spectrum detection on sample microzonal charged molecules, atoms and the like is carried out by utilization of a mass-spectrum detectionsystem; laser mass-spectrum detection and rear spectrophotometric pupil differential confocal detection structures are fused; and thus, high spatial resolution and high-sensitive imaging and detection of sample microzonal composition information and morphological parameters are realized. The invention can provide a brand-new effective technology way for morphological imaging and material composition detection in the fields, such as biomedicines, material sciences, mineral products and micro and nano-fabrication.

Description

technical field [0001] The invention belongs to the field of confocal microscopic imaging technology and mass spectrometry imaging technology, and combines post-divided-pupil laser differential confocal microscopic imaging technology with mass spectrometry imaging technology, and relates to a post-divided-pupil laser differential confocal mass spectroscopic microscopic imaging method and Devices have broad application prospects in biomedicine, materials science, physical chemistry, minerals, micro-nano manufacturing and other fields. Background technique [0002] Mass Spectrometry (Mass Spectrometry) is to ionize the components in the sample, so that the generated charged atoms, molecules or molecular fragments with different charge-to-mass ratios are respectively focused under the action of an electric field and a magnetic field to obtain a sequence of mass-to-charge ratios. Spectrograph instrument. Mass spectrometry imaging is to perform mass spectrometry analysis on mult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64G01N21/63G01N21/01
CPCG01N27/64G01N21/01G01N21/63
Inventor 赵维谦邱丽荣王允
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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