A high-throughput screening and analysis method for organic pollutants in blood

A technology for organic pollutants and analysis methods, which is applied in the field of high-throughput screening and analysis of organic pollutants in blood, can solve problems such as cumbersome processing methods, and achieve the advantages of less sample demand, good application value, and simplified extraction and purification steps. Effect

Active Publication Date: 2020-06-09
NANJING UNIV
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  • Claims
  • Application Information

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Problems solved by technology

[0008] Aiming at the technical problems that the existing detection of organic pollutants in blood is limited to the types of standard substances and blood sample pretreatment methods are cumbersome, the present invention provides a high-throughput screening and analysis method for organic pollutants in blood, which can be accurate, rapid and comprehensive Extraction and purification of organic pollutants in a small amount of blood samples, and obtain screening results of more pollutants that are not limited to standard sample types

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  • A high-throughput screening and analysis method for organic pollutants in blood
  • A high-throughput screening and analysis method for organic pollutants in blood
  • A high-throughput screening and analysis method for organic pollutants in blood

Examples

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Embodiment 1

[0065] In order to verify whether the method for building a library used in the present invention can accurately detect the organic pollutants to be detected, the polluted blood is simulated in Example 1, and different types of organic pollutants are added to the clean blood in a specific amount. After the method was identified, the accuracy of the method was verified by the spectrum of the standard substance.

[0066] The blood sample containing organic pollutants in this example is as follows: take 3 parts of 0.5mL blood samples in a 15mL centrifuge tube, add 50 μL of 30 kinds of organic pollutants with a concentration of 100ppb, vortex and mix them and let stand for 12h, 30 kinds of organic pollutants Substances are common substances widely used in industries such as pesticides, pharmaceuticals, and flame retardants (Table 1).

[0067] A high-throughput screening and analysis method for organic pollutants in blood according to the present invention:

[0068] (1) Extraction...

Embodiment 2

[0100] (1) Extraction of pollutants in blood: take 16 parts of 0.5mL fish blood samples in a 15mL centrifuge tube, add 0.3gMgSO 4 -NaCl mixture (used as SPE) and 1.5mL acetonitrile were mixed on a vortex for 30s (to ensure the infiltration of the SPE material), sonicated for 30min and then centrifuged; the supernatant was transferred to another clean centrifuge tube, and the solid residue was washed with 1.5 mL acetonitrile Repeat the above extraction step twice, and combine the supernatants.

[0101] (2) Concentration and constant volume: Concentrate the extract and slowly purge the solvent with high-purity nitrogen; use 100 μL acetonitrile for constant volume, if there is a small amount of white solid at the bottom after constant volume (during the supernatant transfer process Part of the water phase may move into it, in which saturated MgSO is dissolved 4 and NaCl), it needs to be centrifuged again, and finally the supernatant is transferred to a brown sampling vial and st...

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Abstract

The invention discloses a high-throughput screening analysis method for an organic pollutant in blood, and belongs to the field of chemical analysis. The high-throughput screening analysis method comprises the steps of (1) pollutant extraction; (2) concentration and capacity determination; (3) database building; (4) upper computer detection, in which complete scanning is performed by a high performance liquid chromatography-flight time mass spectrometer under a primary mass spectrum mode and a secondary mass spectrum mode; and (5) substance identification. A pre-treatment method for high-throughput screening the organic pollutant in the blood is built, the compound type extracted under the condition of reducing matrix interference is complete, a few amount of blood samples can be accurately and rapidly extracted and purified by the high performance liquid chromatography-flight time mass spectrometer, a few samples are needed, a primary mass spectrogram and a secondary mass spectrogramof the organic pollutant in the blood are simultaneously, rapidly and continuously scanned, screened and identified in separate assay determination in one time, a standard substance spectrogram is notdepended, the screened organic pollutant is rich in types, many organic pollutants are screened, and the high-throughput screening analysis method has favorable application value.

Description

technical field [0001] The invention belongs to the technical field of environmental analysis chemistry, and in particular relates to a high-throughput screening analysis method for organic pollutants in blood. Background technique [0002] In recent years, with the increasing number of newly synthesized organic compounds, their pollution to the environment has become more and more serious, such as organochlorine pesticides, pharmaceuticals and personal care products (PPCPs), endocrine disruptors (EDCs) and so on. They are ubiquitous in the environment and can enter the human body through breathing, eating, skin contact, etc., and have potential impacts on human health. Blood is the main biological sample for understanding human body contamination caused by chemical factors, so it is extremely important to identify organic pollutants in blood. [0003] However, due to the complex types of organic pollutants, large quantities, different physical and chemical properties, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/08G01N30/72
CPCG01N30/06G01N30/08G01N30/72G01N30/88G01N2030/8813G01N2030/884G01N2030/8845
Inventor 韦斯毛佳迪杜乐天于红霞于南洋叶婷
Owner NANJING UNIV
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