A kind of arbuscular mycorrhizal fungus culture substrate
A technology for arbuscular mycorrhizal fungi and culture substrates, which is applied in the field of microbial cultivation, can solve the problems of high preparation costs and unsatisfactory effects, and achieve the effects of reducing preparation costs and excellent cultivation effects
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Embodiment 1
[0030] An arbuscular mycorrhizal fungus culture substrate, the arbuscular mycorrhizal fungus culture substrate is bentonite pottery sand with a particle size of 0.5mm, and the bentonite pottery sand has been modified, and the modification treatment step is: making the bentonite pottery sand Immerse in the modifying solution, ultrasonically vibrate for 20min under the low pressure condition of 0.3atm, the frequency of ultrasonic oscillation is 30kHz, then take it out and dry it at 75℃ for 3h;
[0031] The above modified solution contains: ferrous sulfate 70mg / L, manganese sulfate 0.1mg / L, copper nitrate 0.03mg / L, zinc nitrate 0.03mg / L, vitamin B 2 10mg / L, vitamin B 3 0.8mg / L, vitamin B 6 0.5mg / L, sucrose 9500mg / L, glucose 2200mg / L, lysine 135mg / L, edetate disodium 150mg / L, sodium molybdate 0.001mg / L and cobalt chloride 0.0001mg / L.
Embodiment 2
[0033] An arbuscular mycorrhizal fungus culture substrate, the arbuscular mycorrhizal fungus culture substrate is bentonite pottery sand with a particle size of 4.5mm, and the bentonite pottery sand has been modified, and the modification treatment step is: making the bentonite pottery sand Immerse in the modifying solution, ultrasonically vibrate for 30min under the low pressure condition of 0.3atm, the frequency of ultrasonic oscillation is 20kHz, then take it out and dry it at 85℃ for 2h;
[0034] The above modified solution contains: ferrous sulfate 80mg / L, manganese sulfate 0.15mg / L, copper nitrate 0.05mg / L, zinc nitrate 0.08mg / L, vitamin B 2 12mg / L, vitamin B 3 1.2mg / L, vitamin B 6 0.9mg / L, sucrose 11000mg / L, glucose 2800mg / L, lysine 170mg / L, edetate disodium 180mg / L, sodium molybdate 0.002mg / L and cobalt chloride 0.0002mg / L.
Embodiment 3
[0036] An arbuscular mycorrhizal fungus culture substrate, the arbuscular mycorrhizal fungus culture substrate is bentonite pottery sand with a particle size of 2.0mm, and the bentonite pottery sand has been modified, and the modification treatment step is: making the bentonite pottery sand Immerse in the modifying solution, ultrasonically vibrate for 25min at a low pressure of 0.3atm, and the frequency of ultrasonic oscillation is 25kHz, then take it out and dry it at 80°C for 2.5h;
[0037] The above modified solution contains: ferrous sulfate 75mg / L, manganese sulfate 0.12mg / L, copper nitrate 0.035mg / L, zinc nitrate 0.05mg / L, vitamin B 2 10.5mg / L, vitamin B 3 1.05mg / L, vitamin B 6 0.65mg / L, sucrose 9800mg / L, glucose 2350mg / L, lysine 150mg / L, edetate disodium 155mg / L, sodium molybdate 0.0015mg / L and cobalt chloride 0.00013mg / L.
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