Fermentation method for producing vitamin B2
A fermentation method and vitamin technology, applied in the field of bioengineering, can solve the problems of high vitamin B2 production, energy saving requirements, insufficient vitamin B2 production, etc., and achieve the effects of reducing fermentation costs, improving fermentation levels, and stabilizing production levels
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Embodiment 1
[0017] Embodiment 1 Configuration of culture medium
[0018] (1) Prepare slant medium, weigh 1.5g of glucose, 0.6g of yeast powder, 0.6g of peptone, 11g of agar powder, prepare 500ml, pH=7.17 before elimination, sterilize at 121°C for 22min, place the slant, and set aside.
[0019] (2) To prepare the liquid medium in the shake flask, weigh 2.2g of glucose, 2g of corn steep liquor, 1.2g of yeast extract, 4g of waste molasses, 0.05g of magnesium sulfate heptahydrate, 0.3g of ammonium chloride, and 0.1g of potassium dihydrogen phosphate, Dipotassium hydrogen phosphate 0.2g, prepare 100ml medium, adjust pH to 7.2, constant volume, dispense into 250ml Erlenmeyer flasks, 18ml per bottle, and sterilize at 121°C for 25min.
[0020] (3) Preparation of seed medium in the primary seed tank, weighing 44g of glucose, 40g of corn steep liquor, 24g of yeast extract, 80g of waste molasses, 1g of magnesium sulfate heptahydrate, 6g of ammonium chloride, 2g of potassium dihydrogen phosphate, dih...
Embodiment 2
[0023] Embodiment 2 A kind of fermentation method of producing vitamin B2
[0024] A fermentation method for producing vitamin B2, comprising the steps of:
[0025] (1) Slant culture: inoculate Bacillus subtilis on the slant medium, and culture in the incubator at 36.5°C for 48 hours;
[0026] (2) Mother bottle culture: Scrape the two-ring bacteria from the slant medium cultivated in step (1) and inoculate it in a shaker flask. The liquid medium in the shaker flask is 100ml. The culture conditions are 210rpm, 37.5°C, and 24h;
[0027] (3) Primary seed cultivation: When the OD value in the shake flask in step (2) has a net increase of 13.6, the inoculum size is 1% into the primary seed tank for cultivation, and the cultivation conditions are tank pressure 0.04Mpa, vvm0.7, tank The temperature is 36.5°C, the rotation speed is 160rpm, and the culture period is 20h;
[0028] (4) Secondary seed cultivation: When the OD value of the first-grade seeds in step (3) has a net increas...
Embodiment 3
[0031] Embodiment 3 A kind of fermentation method of producing vitamin B2
[0032]A fermentation method for producing vitamin B2, comprising the steps of:
[0033] (1) Slant culture: inoculate Bacillus subtilis on the slant medium, and cultivate in a 35°C incubator for 46 hours;
[0034] (2) Mother bottle culture: Scrape the two-ring bacteria from the slant medium cultivated in step (1) and inoculate it in a shake flask with 100ml of liquid medium in the shake flask. The culture conditions are 210rpm, 36.5°C, and 25h;
[0035] (3) Primary seed culture: When the OD in the shake flask in step (2) increases by 12, transfer the inoculum size to 0.8% into a primary seed tank for cultivation, and the cultivation conditions are tank pressure 0.03Mpa, vvm0.6, tank temperature The temperature is 36.5°C, the rotation speed is 150rpm, and the culture period is 21h;
[0036] (4) Secondary seed cultivation: When the OD value of the first-grade seeds in step (3) increases by 12, transfer...
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