Film coating liquid containing aspergillus flavus antagonistic bacteria and preparation method and application thereof

A technology of antagonistic bacteria and coating liquid, which is applied in the field of coating liquid containing antagonistic bacteria of Aspergillus flavus, can solve the problems of reduced efficacy, short duration of effect, contamination of Aspergillus flavus, etc., and achieve the effect of improving prevention

Pending Publication Date: 2019-01-25
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aspergillus flavus is widely distributed and is susceptible to contamination by Aspergillus flavus during peanut planting, harvesting, transportation and storage; most Aspergillus flavus can produce aflatoxin during growth and reproduction; peanuts contaminated with aflatoxin Not only cause harm to economic animals and human body, but also cause waste of resources
[0003] At present, there are many methods of detoxification, detoxification, and degradation of aflatoxin; the coating is to form a protective film on the surface of peanuts, which can effectively isolate exogenous Aspergillus flavus, thereby preventing peanuts from mildewing or being infected by Aspergillus flavus. Toxin pollution; At present, studies have shown that adding some effective plant active ingredients to the coating solution can improve the effect of preventing peanut mildew or contamination by aflatoxin; such as citral, perillaldehyde or garlic essential oil; these active ingredients It has the characteristics of being volatile and short-lasting; microorganisms that antagonize Aspergillus flavus have a good inhibitory effect on the growth, reproduction and toxin production of Aspergillus flavus. However, there are many problems in applying these microorganisms to the biological control of Aspergillus flavus For example: ① apply antagonistic microorganisms to the storage process of agricultural products, and inhibit the infection of Aspergillus flavus by the growth and reproduction of antagonistic microorganisms. During the growth and reproduction of antagonistic microorganisms, they also need to decompose nutrients and consume energy. The agricultural products to be stored may Nutrients have been decomposed by antagonistic microorganisms before being infected by Aspergillus flavus
②In order to prevent the occurrence of the above phenomenon, most studies use the crude extracts of microorganisms to prevent and control Aspergillus flavus. However, most of these microbial crude extracts are active enzymes. Over time, it will be gradually inactivated and its efficacy will be reduced, eventually failing to achieve the effect of preventing and controlling the infection of Aspergillus flavus

Method used

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  • Film coating liquid containing aspergillus flavus antagonistic bacteria and preparation method and application thereof
  • Film coating liquid containing aspergillus flavus antagonistic bacteria and preparation method and application thereof
  • Film coating liquid containing aspergillus flavus antagonistic bacteria and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0029] The preparation of embodiment 1 aspergillus flavus antagonistic bacteria spore

[0030] Aspergillus niger spores: Aspergillus niger AS3.3928 (purchased from Shanghai Beinuo Biotechnology Co., Ltd.) was inoculated on Czapek's agar medium, cultured at 28°C for 5 days, and then the spores on the medium were dipped with cotton swabs in a sterile In 0.1% Tween-80, use an oscillator to oscillate evenly, and then use a hemocytometer to count and adjust its concentration to 10 10 A / mL spare.

Embodiment 2

[0031] The preparation of embodiment 2 Aspergillus flavus antagonistic bacteria extract

[0032] Using Aspergillus flavus antagonistic bacteria Aspergillus niger AS3.3928, Bacillus subtilis, and Saccharomyces cerevisiae as strains, fermented liquid was obtained through seed culture, expanded culture and fermentation production, wherein the number of effective viable bacteria in the fermented liquid was greater than 10 9 cfu / mL;

[0033] Centrifuge the above 400mL fermentation broth, discard the supernatant, and collect the bacteria; wash several times with sterile water, and finally collect the bacteria by centrifugation; add 40mL sterile PBS solution to the bacteria, mix well; add PMSF to the bacteria (1:500 scale). Place in an ultrasonic breaker to crush the bacteria with a power of 70%, break for 3s, stop for 5s, and 7min; centrifuge the broken bacteria at 10,000r / min for 20min, dissolve them with methanol, filter them with suction, and evaporate them to dryness by rotary ...

Embodiment 3

[0034] The inhibitory action of embodiment 3 antagonistic bacteria extracts to Aspergillus flavus

[0035] Sterilize the peanut meal by high-pressure steam, after cooling, divide it into 6 groups, each group has 3 parallels, and each parallel is 50g; add sterilized water to it, and make its water content reach 50%; add Aspergillus flavus to it Spore (ATCC 28539, purchased from Beijing Bei Nachuanglian Biotechnology Research Institute), the addition amount is 10 7 each / 50g; antagonistic bacteria extracts were added to the above six groups, and the added amounts were 0%, 0.05%, 0.1%, 0.3%, 0.5% and 1.0%. After mixing evenly, ferment at 30°C for 72 hours, stirring and keeping moist during the test; after the test, the total amount of Aspergillus flavus is determined as follows: figure 1 shown.

[0036] Depend on figure 1 It can be seen that the number of Aspergillus flavus in peanut meal without adding antagonistic bacteria extract increased slightly, and the number of Aspergi...

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Abstract

The invention discloses a film coating liquid containing aspergillus flavus antagonistic bacteria and a preparation method and application thereof, and belongs to the technical field of food safety and processing. The film coating liquid containing aspergillus flavus antagonistic bacteria is prepared by adding spores of antagonistic bacteria and / or crude extract of antagonistic bacteria into a film coating agent solution, wherein the antagonistic bacteria is selected from at least one of aspergillus niger, bacillus subtilis and saccharomyces cerevisiae; the adding amount of the spores of antagonistic bacteria is 106 to 108 spores per liter; the adding amount of crude extract of antagonistic bacteria is from 0.1% to 0.5%. The film coating liquid has the advantages that the pollution to peanut by aflatoxin can be effectively prevented; under the synergistic function of aspergillus niger spores and extract of antagonistic bacteria, the effect of preventing the pollution by aspergillus flavus toxin is realized.

Description

technical field [0001] The invention belongs to the technical field of food safety and processing, and in particular relates to a coating solution containing an antagonistic bacteria of Aspergillus flavus, a preparation method and application thereof. Background technique [0002] Peanut is an important oil crop and economic crop. The planting area of ​​peanut in my country is 5 million hectares, and the annual output of peanut is 14 million tons, accounting for more than 40% of the world's total peanut production. Aflatoxins are mainly secondary metabolites produced by Aspergillus flavus (A.flavus) and Aspergillus parasiticus (A.parasiticus), which are derivatives of dihydrofuranocoumarins, and were approved by the World Health Organization (WHO) in 1993. Cancer Research Institute delineated as a class I carcinogen. Aspergillus flavus is widely distributed and is susceptible to contamination by Aspergillus flavus during peanut planting, harvesting, transportation and stora...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B9/14A23B9/28A23B9/26A23B9/24
CPCA23B9/14A23B9/24A23B9/26A23B9/28A23V2002/00
Inventor 朱英莲杨庆利赵海燕唐娟于春娣
Owner QINGDAO AGRI UNIV
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