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Application and products of manganese-type high-stable superoxide dismutase in improving autism

A high-stability, superoxide technology, applied in the medical field, can solve the problem of ASD without a treatment plan, and achieve the effect of alleviating and improving autism behavior, improving ecological imbalance, and improving oxidative stress

Active Publication Date: 2022-04-05
HANGZHOU REDOX PHARMATECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no better treatment plan for ASD. Such a severe situation is a great burden to the family and the society.

Method used

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  • Application and products of manganese-type high-stable superoxide dismutase in improving autism
  • Application and products of manganese-type high-stable superoxide dismutase in improving autism
  • Application and products of manganese-type high-stable superoxide dismutase in improving autism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the preparation of manganese type high stability superoxide dismutase (MS-SOD)

[0029] Using the genome of thermophilic bacteria HB27 (purchased from ATCC cell bank in the United States, the preservation number is ATCC BAA-163) as a template, the target gene was amplified with the following primer sequence: Forward primer: 5'-agaattcatgccgtacccgttcaagct-3' (SEQ ID NO: 1) reverse primer: 5'-ctgtcgactcaggccttcttgaagaac-3' (SEQ ID NO: 2); the amplified product was recovered with a recovery kit (purchased from Sangon Bioengineering Shanghai (Share) Co., Ltd.), and the enzymes EcoRI and Sal I double enzyme digestion, and connected to the plasmid vector pET28a (+) (purchased from Sangon Bioengineering Shanghai (Co., Ltd.)) with the same enzyme double enzyme digestion, and the recombinant plasmid was transformed into competent E. coli BL21 (DE3) (purchased from Sangon Bioengineering Shanghai (Stock) Co., Ltd.), the strain with the correct MS-SOD nucleotide seque...

Embodiment 2

[0033] Embodiment 2: Construction of autism mouse model

[0034] (1) Source of mice: Adult C57 female mice and male mice were purchased from Guangdong Provincial Experimental Animal Center. After feeding for 8 weeks, feed 30 female mice (20-25g) and male mice (25-30g) under the conditions of periodic light (7:00AM~7:00PM), constant temperature 25°C and constant humidity 55%. Allow it to acclimatize for a few days. Then they were caged overnight at a ratio of 1:1 for males and females. On the next morning, the female mice whose vaginal plugs were detected were recorded as E1 (the first day of the embryo, embryoic 1), and then separated into cages and reared individually. The pregnant female mice were randomly divided into groups.

[0035] (2) Autism mouse model:

[0036] VPA (sodium valproate) modeling is one of the recognized modeling methods, which is often used in the research and treatment of epilepsy and other neuropsychological disorders.

[0037] VPA model (sodium v...

Embodiment 3

[0039] Embodiment 3: test experiment

[0040] 1. Grouping of MS-SOD intervention experiments:

[0041] The mice used in this experiment (the offspring of the above-mentioned pregnant mice) were randomly divided into the following groups (10 mice in each group) after three weeks of weaning, and the intervention was continued for 4 weeks, and feces were collected every week. The eighth week began to detect behavior (SuperMaze animal behavior experiment analysis software). After the test was completed, the mice were anesthetized with chloral hydrate and dissected, and the intestinal tissue and brain tissue were collected.

[0042] Group A: normal mice (NC group): drinking water freely;

[0043] Group B: normal mice + MS-SOD intervention group (NC + MS-SOD group): drinking MS-SOD solution (30U / 10g body weight);

[0044] Group C: autistic mice (ASD group): free drinking water;

[0045] Group D: autistic mice + MS-SOD intervention group (ASD + MS-SOD group): drinking MS-SOD soluti...

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Abstract

The present invention provides the application of the manganese-type high-stable superoxide dismutase whose sequence is shown as SEQ ID NO: 4 in the treatment of autism. The manganese-type high-stable superoxide dismutase used in the present invention can improve the ecological imbalance of the intestinal flora, regulate the expression of intestinal shielding-related proteins, thereby improving the oxidative stress of the intestinal tract and brain, and relieve and improve autism Behavioral purposes offer new avenues for the treatment of autism.

Description

technical field [0001] The invention belongs to the medical field and relates to the improvement and treatment of autism symptoms. Background technique [0002] Autism spectrum disorder (ASD) is a pervasive developmental disorder characterized by varying degrees of social and communication impairments, repetitive and stereotyped behaviors. The severity of the disease varies from individual to individual, and it is often combined with one or several other diseases, including mental retardation, anxiety, and gastrointestinal symptoms. The etiology of ASD is unclear, and currently proposed mechanisms include [1-8] 1) Gene mutations cause polymorphisms in some specific genes; 2) Epigenetic variation in gene expression; 3) Environmental pollution; 4) Pathogen infection during pregnancy; 5) Gastrointestinal metabolic dysfunction; 6 ) Immunoregulation; 7) Imbalances in gut-brain connectivity; 8) Oxidative stress; 9) Mitochondrial dysfunction; 10) Neuroinflammation during developm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/44A61P25/00A23L33/10
CPCA61K38/446A61P25/00A23L33/10C12Y115/01001A23V2002/00A23V2200/32A23V2200/322
Inventor 孟凡国
Owner HANGZHOU REDOX PHARMATECH CO LTD
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