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Industrial utilization method for stevia, stevioside and chlorogenic acid

A technology of steviol glycosides and stevia, applied in chemical instruments and methods, separation/purification, and application of carboxylic acid esters, can solve problems such as inability to obtain stevia chlorogenic acid, affect the quality of glycoside products, and fail to meet market requirements , to achieve effective separation, improve purity and quality, improve efficiency and life

Active Publication Date: 2019-02-01
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition to glycosides and flavonoids in the alcohol extract, some low-polarity impurities will also be extracted at the same time, and the impurities are not removed before the resin is purified, which will lead to rapid pollution of the resin, and the stevia alcohol extract contains some low-polarity impurities. Impurities, the analysis process of low-alcohol solution can only remove high-polarity impurities. When high-alcohol solution is used for analysis, low-polarity impurities enter the glycoside analysis solution, affecting the quality of glycoside products, which cannot meet market requirements, and the patent cannot obtain stevia green Ortho acid

Method used

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  • Industrial utilization method for stevia, stevioside and chlorogenic acid

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Embodiment 1

[0057] This embodiment relates to a method for industrialized utilization of stevia, comprising the following steps:

[0058] (1) Take 1 kg of stevia powder by weighing, use 85% ethanol aqueous solution as extracting liquid, repeat extraction 3 times to the powder of described stevia under the condition of 50 ℃, the solid-liquid ratio in 3 extraction processes is respectively 1 : 5 / 3.5 / 3.5, the 1st extraction time is 1.5h, the 2nd and 3rd extraction time is 1h, and the combined filtrate is used as extracting solution;

[0059] (2) Concentrate the extract to 1 / 10 of the original volume in a water bath at 60°C and vacuum-0.08MPa, add an equal volume of n-hexane to the concentrate for liquid-liquid extraction for 3 times, take the water layer to obtain Extraction solution with low polarity impurities;

[0060] (3) Adjust the pH of the extract to remove low-polar impurities to 10.0, and adjust the solid content to 10%, then adsorb it through 1.5L of T28 resin, and adjust the flow...

Embodiment 2

[0063] This embodiment relates to a method for industrialized utilization of stevia, comprising the following steps:

[0064] (1) Take by weighing 1 kg of stevia powder, use 95% methanol aqueous solution as extracting liquid, repeat extraction 2 times to the powder of described stevia under the condition of 50 ℃, the solid-liquid ratio in 2 extraction processes is respectively 1 : 6 / 4.5, the 1st extraction time is 1.5h, the extraction time of the 2nd time is 1h, and the combined filtrate is used as extractant;

[0065] (2) Concentrate the extract to 1 / 10 of the original volume in a water bath at 60°C and a vacuum of -0.08MPa, add an equal volume of diethyl ether to the concentrate for liquid-liquid extraction for 3 times, take the water layer, and obtain the low Extraction of polar impurities;

[0066] (3) Adjust the pH of the extract to remove low-polarity impurities to 9.5, and adjust the solid content to 8%, which is adsorbed by 1.5L of 201-H resin, and the flow rate of th...

Embodiment 3

[0069] This embodiment relates to a method for industrialized utilization of stevia, comprising the following steps:

[0070] (1) Take by weighing 1 kg of stevia powder, use 75% propanol aqueous solution as extracting liquid, repeat extraction 2 times to the powder of described stevia under the condition of 50 ℃, the solid-liquid ratio in 2 extraction processes is respectively 1:6 / 4.5, the extraction time for the first time is 1.5h, the extraction time for the second time is 1h, and the combined filtrate is used as the extraction solution;

[0071] (2) Concentrate the extract to 1 / 10 of the original volume in a water bath at 60° C. and a vacuum of -0.08 MPa, add an equal volume of cyclohexane to the concentrate for liquid-liquid extraction for 3 times, and take the water layer to obtain Extraction solution to remove low-polarity impurities;

[0072] (3) Adjust the pH of the extract to remove low-polarity impurities to 9.0, and adjust the solid content to 6%. It is adsorbed by...

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Abstract

The invention relates to an industrialization method for comprehensive utilization of stevia. The method is mainly characterized in that the stevia is extracted with a high-concentration alcohol solution, then the obtained extract is purified by using an organic solvent, and the extract is adjusted to be alkaline according to the acidic characteristic of chlorogenic acid to make the chlorogenic acid form a salt and have an increased polarity in order to realize effective separation of the chlorogenic acid and a glucoside component in the adsorption link. The method allows the high-quality stevioside and chlorogenic acid to be obtained, significantly improves the comprehensive utilization rate of stevia, reduces the waste of natural stevia resources, reduces the resource consumption in theproduction process, reduces waste discharge, is a high-benefit green production process, and can greatly advance the industry.

Description

technical field [0001] The invention relates to the field of extracting active components in plants, in particular to an industrialized method for the comprehensive utilization of chlorogenic acid and stevioside in stevia rebaudiana. Background technique [0002] Stevia rebaudiana (Stevia rebaudiana) is a perennial herb of Compositae, native to Paraguay and Brazil in South America. It is one of the known sugar plants with high sweetness and has become the third natural sugar after sucrose and beet sugar. source. At present, China is the world's largest producer and supplier of steviol glycosides, accounting for more than 80% of the global total. In addition to steviol glycosides, stevia also contains 3-6% chlorogenic acid (HPLC), of which isochlorogenic acid accounts for nearly 80%, and isochlorogenic acid has important biological activities, such as anti-inflammatory and anti-viral , anti-oxidation, lowering blood pressure, lowering blood lipids, etc. It has been used as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H15/256C07C67/48C07C69/732
CPCC07C67/48C07H1/08C07H15/256C07C2601/14C07C69/732C13K13/007A23L27/30C07C67/56C07H1/00C07H15/24
Inventor 徐美利连运河田洪高伟徐亚超
Owner CHENGUANG BIOTECH GRP CO LTD
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