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Preparation method of ultralow molecular weight yak collagen peptide

A technology of molecular weight and collagen peptide, which is applied in the field of preparation of ultra-low molecular weight yak collagen peptide, can solve the problems of ultrasonic extraction method, high mass production of chromatography method, large sewage discharge, etc., and achieve green process and high molecular weight. The effect of low cost and simple process

Inactive Publication Date: 2019-02-01
西藏银美科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Typically, for example, the process of a preparation method of yak collagen (application number CN 106148466A) is to crush yak tissue, enzymatic hydrolysis, and then use chromatography to separate and purify collagen peptides from the interstitial fluid obtained by enzymatic hydrolysis, although it can be obtained Collagen, but the high cost of high-volume production of chromatography in industry is higher; A kind of technical process in the fractional extraction process (application number CN107446981A) of bovine collagen peptide is that ultrasonic wave carries out once extraction solid-liquid separation, obtains filtrate, The filter residue is enzymatically hydrolyzed once to obtain a primary enzymatic hydrolysis solution to kill enzymes, separate insolubles, and remove impurities to obtain a refined solution that is concentrated, sterilized, and dried to obtain primary protein peptide powder; Separation of insoluble matter and removal of impurities to obtain secondary refined liquid Concentration treatment for sterilization and drying to obtain secondary protein peptide powder. Although the process used is free of acid and alkali pollution and emissions, it is difficult to realize the ultrasonic extraction method in industry
As mentioned above, in the existing collagen peptide extraction process, there are generally problems such as cumbersome extraction process and / or large amount of sewage discharge. Preparation process developed for environmental concerns

Method used

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  • Preparation method of ultralow molecular weight yak collagen peptide
  • Preparation method of ultralow molecular weight yak collagen peptide
  • Preparation method of ultralow molecular weight yak collagen peptide

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Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1: the depilatory effect comparison of yak skin

[0034] Adopt and compare the hair removal effect of chemical hair removal and this patent hair removal method, the method is as follows: (1) Referring to the chemical hair removal method in patent 201710089525.0, according to the ratio of 100 parts of water, 12 parts of sodium sulfide and 30 parts of slaked lime are mixed to prepare a depilatory cream, and then the yak The skin is laid flat on the ground, with the skin facing down and the hair facing up, and the depilatory cream film prepared is applied on the hair surface. After stacking for 5 hours, the hair is manually pushed and rinsed; (2) in this patent, the yak skin is decontaminated and cleaned. Repeated cleaning, soaking with 0.1-1% (w / v) NaOH for 1 hour; repeated cleaning after soaking. The processed yak skin was processed under 0.1MP high temperature and high pressure conditions for 1 hour, and after processing, it was filtered while hot to remove h...

Embodiment 2

[0037] Embodiment 2: Preparation of yak collagen peptide

[0038] Take the yak skin and wash it repeatedly, soak it with 0.5% (w / v) NaOH for 2 hours, and wash it repeatedly after soaking, then treat the cleaned yak skin at 0.08mP high pressure and 115°C for 3 hours. Heat filtration, refrigerate overnight at 4°C, filter with gauze to remove grease, collect the filtrate, add 2g of bromelain per 25g of tissue, enzymatically hydrolyze at 35°C for 6 hours, then add 2g of papain per 25g of tissue, and enzymolyze at 35°C for 6h, enzymatically After the hydrolysis, heat to 80°C to inactivate the enzyme, centrifuge, concentrate, and dry. And the molecular weight of the prepared collagen peptide was detected. See the test results figure 1 and Table 2. The results showed that the content of collagen peptides with molecular weight less than 300d accounted for 75%.

[0039] Table 2 GPC detection results of yak collagen peptide

[0040]

Embodiment 3

[0042] Take the yak skin and wash it repeatedly, soak it with 0.1% (w / v) NaOH for 3 hours, wash it repeatedly after soaking, and treat the cleaned yak skin at a high pressure of 0.09 mP and a high temperature of 120°C for 5 hours. Heat filtration, refrigerate overnight at 4°C, filter with gauze to remove grease, collect the filtrate, add 1g of bromelain per 10g of tissue, enzymolyze at 30°C for 8 hours, then add 1g of papain per 10g of tissue, enzymolyze at 30°C for 8h, enzymatically After the hydrolysis, heat to 80°C to inactivate the enzyme, centrifuge, concentrate, and dry. Obtain ultra-low molecular weight yak collagen peptide.

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Abstract

Belonging to the technical field of nutritional health products, the invention discloses a preparation method of an ultralow molecular weight yak collagen peptide. The preparation method of the ultralow molecular weight yak collagen peptide includes: conducting dilute alkali soaking, high temperature and high pressure pulping, filtration, enzymolysis, concentration, spray drying and other processes on yak hide to obtain yak hide collagen peptide hydrolysate, the ultralow molecular weight collagen peptide (less than 300D) content of which is greater than 75%. The ultralow molecular weight yak collagen peptide prepared by the method provided by the invention can combine with a mannose receptor on macrophage to stimulate macrophage proliferation and enhance immunity.

Description

technical field [0001] The invention relates to a preparation method of ultra-low molecular weight yak collagen peptide, which belongs to the technical field of nutrition and health care products. Background technique [0002] Yak is a unique breed of cattle on the Qinghai-Tibet Plateau. Studies have shown that yak skin collagen has many advantages such as high content and strong biological activity. In particular, ultra-low molecular weight peptides have been proven to pass directly through the intestinal wall and enter the blood circulation without decomposition, so that they can be directly and completely absorbed by the human body, so the effect of eating is very good. Utilizing yak skin to produce collagen or collagen peptide, especially the production of ultra-low molecular weight collagen peptide, can realize high-value utilization of yak skin. [0003] The main process of the traditional collagen peptide preparation method is depilation-degreasing-cooking-mincing-...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/34
CPCC07K14/78C12P21/06
Inventor 范小剑闻献蒋敏史劲松许正宏
Owner 西藏银美科技股份有限公司
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