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Preparation method and application of biological seedling raising substrate of chaetomium globosum

A seedling-raising substrate and the technology of Chaetomium globosa, applied in the field of agricultural microorganisms, can solve the problems of reduced control effect, high cost of chemical control, low risk, etc., achieve huge economic and social benefits, promote healthy development, and use simple methods Effect

Active Publication Date: 2019-02-12
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because there are few varieties of high-quality, high-yield and disease-resistant vegetables in the production of facility vegetables, and the breeding cycle of disease-resistant varieties is long, so agronomic measures such as grafting, chemical control and biological control are often used to control Fusarium wilt
Grafting cultivation and soil chemical treatment can effectively prevent and control the occurrence of vegetable wilt, but with the large-scale promotion of grafting cultivation in China, the decline of rootstock resistance and improper management measures have increased the incidence of grafted seedlings Fusarium wilt year by year (Zhang Lihua , 2008); the cost of chemical agent control is high and labor intensive, and long-term use can easily cause pathogenic bacteria to develop drug resistance, leading to a decline in the control effect, and at the same time causing pesticide residues and air pollution (Faheem M, 2015); biological control methods are environmentally friendly, The risk is low, but the potential has not been fully utilized (Tikhonovich IA, 2011). Some biocontrol bacteria with high control effects on soil-borne diseases such as fusarium wilt and damping-off that were screened under laboratory conditions are affected by the environment in the field. Larger, the number of colonization is often reduced, and the effect of disease prevention is significantly weakened (Wu Xiaoqing, 2017)

Method used

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  • Preparation method and application of biological seedling raising substrate of chaetomium globosum
  • Preparation method and application of biological seedling raising substrate of chaetomium globosum
  • Preparation method and application of biological seedling raising substrate of chaetomium globosum

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preparation example Construction

[0054] In a typical embodiment of the present invention, a kind of preparation method of Chaetomium globosa biological seedling-raising matrix is ​​provided, and described method comprises:

[0055] After the primary fermentation of the agricultural waste, the Chaetomium globosa ND35 sexual spore powder is inoculated therein for secondary fermentation to obtain the Chaetomium globosa biological seedling substrate.

[0056] Wherein, the agricultural waste includes but not limited to bark, sawdust, straw, etc.;

[0057] In yet another typical embodiment of the present invention, the agricultural waste is a mixture of pulverized poplar bark, poplar wood chips and corn stalks, and the mass ratio of the poplar bark, poplar wood chips and corn stalks is 6-6. 10:6~10:1~3 (preferably 7:8:2); Studies have shown that the use of agricultural waste in the above mixing ratio range can significantly improve the activity of Chaetomium globosa ND35 in the secondary fermentation process, Not ...

Embodiment 1

[0081] Embodiment 1 nursery substrate formula screening

[0082] 1. Raw materials

[0083] Fresh poplar bark, poplar sawdust, crop straw such as wheat and corn.

[0084] 2. Raw material screening

[0085] (1) Single raw material screening According to the basic indicators of raw materials and their effects on the growth of vegetables such as cucumbers and tomatoes, determine the main raw materials of the seedling substrate, and prepare substrates suitable for the growth of cucumber and tomato seedlings.

[0086] (2) Screening of substrate formula Determine the main raw materials on the basis of the previous step, and configure seedling substrates with different formulas by adding different auxiliary materials, and observe their effects on the growth of cucumber and tomato.

[0087] 3. Plant growth indicators

[0088] When the plants had 5 leaves and 1 heart, the morphological indicators such as plant height, stem diameter, root-to-shoot ratio, and physiological and biochemi...

Embodiment 2

[0093] The preparation method of embodiment 2 Chaetomium globosa biological seedling-raising matrix

[0094] 1. Raw materials

[0095] Fresh poplar bark, poplar wood chips, corn stalks, humic acid, brown sugar.

[0096] 2. Primary fermentation of raw materials

[0097] Stack fresh poplar bark, poplar wood chips, and corn stalks (the mass ratio of the three is 7:8:2) into a fermentation pile of 5×5m (the stacking area can be adjusted according to the actual situation), and cover it with a film to maintain a relatively closed environment . The fermentation humidity of the stockpile is maintained by adding an aqueous solution of brown sugar (red sugar content is 0.5%), the fermentation temperature is controlled at 60°C-80°C, the fermentation humidity is 55%-65%, and the fermentation is continued for 24 days.

[0098] 3. Secondary fermentation of raw materials

[0099] The raw materials obtained by primary fermentation are sterilized. Pass steam into the primary fermentation ...

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Abstract

The invention provides a preparation method and application of a biological seedling raising substrate of chaetomium globosum, and belongs to the technical field of agricultural microorganisms. The method comprises the steps that after preliminary fermentation is performed on agricultural waste, the agricultural waste is inoculated with chaetomium globosum ND35 sexual conidial powder to perform secondary fermentation, so that a functional flora taking chaetomium globosum as the main microorganisms in the substrate, and the biological seedling raising substrate of chaetomium globosum is obtained. The agricultural waste comprises, but is not limited to, bark, wood chips, straw and the like. The preparation method and application of the biological seedling raising substrate of chaetomium globosum have the advantages of being simple, being capable of being directly applied to the seedling raising stages of tomatoes, cucumbers and other plants, planting chaetomium globosum ND35 bacterial strains into the plants more directly and quickly, and being beneficial to the plants perpetually; the biological seedling raising substrate of chaetomium globosum is an excellent seedling raising substrate, can be applied to industrialized seedling raising of various plants, fills in the blank of domestic excellent substrates, replaces imported substrates, promotes the healthy development of industrialized seedling raising of vegetables, and has great economic and social benefits.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and relates to a preparation method and application of a chaetomium globosum biological seedling-raising substrate, in particular to a preparation method of a biological seedling-raising substrate using ascospores of the Chaetomium globosum ND35 strain as active ingredients . Background technique [0002] The statements in this section merely provide background information related to the present disclosure and do not necessarily constitute prior art. [0003] Chaetomium globosa (C.globosum) ND35 strain is an endophytic fungus isolated from healthy Populus tomentosa, which belongs to Ascomycota, Sclerotinia, Globosum, Nigeriaceae, and Chaetomycetes. Existing test results show that Chaetomium globosa ND35 strain can effectively colonize vegetable plants such as cucumber, tomato, kidney bean and field crops such as sweet potato, wheat, potato, tobacco, ginger, and has a strong e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G24/20A01G24/23A01G24/22A01G24/28
CPCA01G24/20A01G24/22A01G24/23A01G24/28
Inventor 高克祥张蕊田叶韩刘晓光何邦令
Owner SHANDONG AGRICULTURAL UNIVERSITY
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