Fuzhuan tea polyphenol and polysaccharide composite instant tea and preparation method and application thereof

A Fuzhuan brick tea, instant tea technology, applied in the application, tea, tea extraction, etc., can solve the problems of difficult to remove tea caffeine, different separation and purification methods, low extraction rate of tea polysaccharides, etc., to achieve excellent solubility, improve purity, The effect of increasing the extraction rate

Inactive Publication Date: 2019-02-15
SHAANXI NORMAL UNIV
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Problems solved by technology

[0006] At the same time, in the existing tea polyphenols or tea polysaccharide preparation methods, it is difficult to remove the caffeine contained in tea, and the extraction rate of tea polysaccharides is low; polysaccharides are hydrophilic macromolecules, and the separation and purification method of small molecules Different, and different extraction methods for the same plant, the obtained polysaccharides will not only have different structures, but also their biological activities will change, so it is necessary to use a suitable method to separate and purify Fuzhuan tea polyphenols and tea polysaccharides

Method used

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  • Fuzhuan tea polyphenol and polysaccharide composite instant tea and preparation method and application thereof
  • Fuzhuan tea polyphenol and polysaccharide composite instant tea and preparation method and application thereof
  • Fuzhuan tea polyphenol and polysaccharide composite instant tea and preparation method and application thereof

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preparation example Construction

[0055] The invention discloses a composite instant tea of ​​Fuzhuan tea polyphenols and polysaccharides and a preparation method thereof. The Fuzhuan tea is prepared by compounding tea polyphenols and tea polysaccharides obtained after purification. The preparation process of the Fuzhuan tea includes the following steps:

[0056] (1) Preparation of Fuzhuan Tea Polyphenols

[0057] (1-1) Pulverize Fuzhuan tea leaves, add them into ethanol with a mass concentration of 70-85% according to the ratio of solid to liquid: 1g: 50mL, stir well and then leaching for 1.5h to obtain mixture A;

[0058] (1-2) filtering the mixture A obtained in step (1-1), taking the filtrate to obtain filtrate B;

[0059] (1-3) Repeat step (1-2) 2 to 3 times, combine filtrate B and then sterilize the filtrate to obtain filtrate C; sterilization includes pasteurization and other sterilization methods.

[0060] (1-4) Plate and frame press filtration filtrate C further removes impurities to improve the extr...

Embodiment 1

[0078] For the compound tea prepared in Example 1, the monosaccharide composition of the polysaccharide component of Fuzhuan brick tea was determined by HPLC-UV detection technology, and its elution curve and related data analysis results are shown in Table 1 respectively. figure 2 It can be seen that the 10 kinds of monosaccharides and Fuzhuan tea polysaccharide samples can maintain a good baseline separation within 40 minutes. Comparing the retention time of the monosaccharide standard in the elution curve can determine the monosaccharide composition in Fuzhuan brick tea polysaccharide, and the mole percentage of each monosaccharide in the sample can be calculated according to the peak area of ​​each elution peak, of which mannose (14.71%), rhamnose (1.13%), glucuronic acid (9.87%), galacturonic acid (5.49%), glucose (25.69%), xylose (2.42%), galactose (37.52%), Arabinose (3.17%).

[0079] Table 1. Monosaccharide composition and corresponding mole percentage of Fuzhuan bri...

Embodiment 2

[0093] (1) Preparation of Fuzhuan Tea Polyphenols

[0094] Pulverize the Fuzhuan tea leaves, after the Fuzhuan tea leaves pass through a 60-mesh standard inspection sieve, add them into ethanol with a mass concentration of 82% according to the material-to-liquid ratio of 1:50, fully stir and extract for 1.5 hours; filter the extract and take the filtrate; Repeat the extraction twice, combine the filtrate, and heat the filtrate to 80°C for pasteurization; the sterilized liquid is further removed by plate and frame press filtration to further remove impurities, and then ultrafiltration is used to remove macromolecules with Mw>10000Da, and the filtrate is collected to obtain filtrate D; The collected filtrate was subjected to double-effect concentration and then freeze-dried to obtain crude tea polyphenols; after the crude tea polyphenols were dissolved in distilled water, the resulting solution was chromatographed with a macroporous resin with a mass ratio of 1:1, and the loading...

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Abstract

The invention discloses Fuzhuan tea polyphenol and polysaccharide composite instant tea and a preparation method and application thereof. The instant tea contains the following components in percentage by mass of 30-85% of tea polyphenols and 15-70% of tea polysaccharide, wherein the tea polysaccharide can promote absorption of the tea polyphenols in human bodies and strengthen the physiological activity of the tea polyphenols, and the utilization rate of the tea polyphenols is increased. The tea polyphenols in the Fuzhuan tea polyphenol and polysaccharide composite instant tea disclosed by the invention are obtained through separation and purification of Fuzhuan tea leaves, Fuzhuan tea leaves are used as raw materials, and pressure filtration is performed through a sheet frame to remove impurities efficiently, so that the refining degree is increased; through macroporous resin chromatography, low-activity phenolic acid compounds generated during extraction of the tea polyphenols are removed, and besides, impurities having potential side effects, including caffein and the like, are removed, so that the Fuzhuan tea polyphenol and polysaccharide composite instant tea is also suitablefor crowds suffering from insomnia easily while being suitable for common crowds. The preparation method disclosed by the invention is simple and convenient, low in cost and high in added value, andis suitable for popularization and usage.

Description

【Technical field】 [0001] The invention belongs to the technical field of tea products and food engineering, and in particular relates to a compound instant tea made of Fuzhuan tea polyphenols and polysaccharides and its preparation method and application. 【Background technique】 [0002] Tea originated in China at the earliest, and there have been thousands of years of tea planting, tea making, tea drinking and tea culture in China. Tea has the effects of fitness and disease prevention, and has become the most popular medicinal drink in the world today. [0003] Studies have shown that many active components in tea have the function of life protection, and tea polyphenols and tea polysaccharides are the main active components of tea to exert physiological functions. Tea polyphenols account for 25-35% of the weight of tea, accounting for 15% of the dry weight of tea. ~30%, is the general term for polyphenols in tea, including flavanols, anthocyanins, flavonoids, flavonols and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/16A23F3/18A23F3/26
CPCA23F3/16A23F3/18A23F3/26
Inventor 杨兴斌杜海平王琦
Owner SHAANXI NORMAL UNIV
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