Deep-sea fish skin collagen peptide

A technology for fish skin collagen and skin collagen, which is applied to animal/human proteins, specific peptides, connective tissue peptides, etc., can solve the problems of complicated preparation process and low collagenase hydrolysis efficiency, and achieves high extraction efficiency and simple and easy preparation method. line, the effect of small molecular weight

Inactive Publication Date: 2019-02-15
兰溪市哥特生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantage of this method is that the preparation process is complicated and the enzymatic hydrolysis efficiency of collagen is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A deep-sea fish skin collagen peptide, the preparation method comprising fish skin pretreatment, fish skin collagen extraction, fermentation broth preparation, fish skin collagen peptide preparation, and fish skin collagen peptide purification, the specific steps are as follows:

[0023] 1) Fish skin pretreatment: take cod skin as raw material, wash the fish skin in ice water, cut it into pieces, then put it in a solution containing a degreasing agent, stir continuously at a speed of 180rpm for 36h, and control the ratio of solid to liquid 1:20, degreasing temperature 12°C, then soak the degreased fish skin with 0.05mol / L NaOH solution for 50min, and finally wash the soaked fish skin with water until neutral, drain the water for later use, Pretreatment can remove the fat wrapped around the collagen fibers and non-collagenous proteins such as globulin and keratin, which facilitates subsequent extraction and separation, and is conducive to improving the purity of fish skin...

Embodiment 2

[0034] A deep-sea fish skin collagen peptide, the preparation method comprising fish skin pretreatment, fish skin collagen extraction, fermentation broth preparation, fish skin collagen peptide preparation, and fish skin collagen peptide purification, the specific steps are as follows:

[0035] 1) Fish skin pretreatment: take cod skin as raw material, wash the fish skin in ice water, cut it into pieces, and then place it in a mixture of isopropanol and ether with a volume ratio of 1:1.5 at a speed of 200rpm Stir continuously for 40 hours, control the ratio of solid to liquid to 1:25, and degrease the temperature at 12°C, then soak the degreased fish skin in 0.1mol / L NaOH solution for 50 min, and finally wash the soaked fish skin repeatedly with water until neutral , drain the water for later use;

[0036] 2) Extraction of collagen from fish skin: Swell the pretreated fish skin with 0.8mol / L glacial acetic acid solution for 40 minutes, control the ratio of solid to liquid to 1:...

Embodiment 3

[0044] A deep-sea fish skin collagen peptide, the preparation method comprising fish skin pretreatment, fish skin collagen extraction, fermentation broth preparation, fish skin collagen peptide preparation, and fish skin collagen peptide purification, the specific steps are as follows:

[0045] 1) Fish skin pretreatment: take cod skin as raw material, wash the fish skin in ice water, cut it into pieces, and then place it in a mixture of isopropanol and ether with a volume ratio of 1:1.2 at a speed of 180rpm Stir continuously for 36 hours, control the ratio of material to liquid to 1:23, and degrease the temperature at 8°C, then soak the degreased fish skin with 0.08mol / L NaOH solution for 30 minutes, and finally wash the soaked fish skin repeatedly with water until neutral , drain the water for later use;

[0046] 2) Extraction of collagen from fish skin: Swell the pretreated fish skin with 0.6mol / L glacial acetic acid solution for 30 minutes, control the ratio of material to ...

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PUM

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Abstract

The invention discloses deep-sea fish skin collagen peptide. A preparation method of the deep-sea fish skin collagen peptide comprises the following steps: firstly, performing degreasing and impure protein removal on washed deep-sea fish skin, and extracting fish skin collagen by combining acid soaking and water bath extraction; then, inoculating activated lactic acid bacteria into fermentation liquor containing fish skin collagen powder for culture and fermentation, controlling fermentation time, temperature and pH in the fermentation process, performing filtering and centrifugation after fermentation, taking supernatant to obtain a fish skin collagen peptide solution, decolorizing and deodorizing the fish skin collagen peptide solution, finally, performing ultrafiltering, rotarily evaporating and concentrating filtrate, and performing freezing at low temperature and vacuum drying to obtain the fish skin collagen peptide. The deep sea fish skin collagen peptide has high purity, low molecular weight, good biological activity and high hydrophilicity, and the preparation method of the deep-sea fish skin collagen peptide is simple and easy to implement, mild in reaction conditions andhigh in extraction efficiency, and facilitates commercial popularization.

Description

technical field [0001] The invention relates to the technical field of marine bioengineering, in particular to a deep-sea fish skin collagen peptide. Background technique [0002] In recent years, with the improvement of living standards, the diversity and practicability of people's demand for aquatic resources have gradually increased, thus promoting the rapid development of my country's aquatic product processing industry. However, with the rapid development of the aquatic product processing industry, a large amount of fish leftovers, such as fish skin, fish scales, viscera, fish heads, etc., will be produced. Most of the feed is discarded, which not only causes a great waste of resources, but also causes environmental pollution. Therefore, how to efficiently utilize the nutritionally active substances in fish scraps has attracted great attention from scientific researchers at home and abroad. Among them, the main waste fish skin is rich in collagen, and collagen can acco...

Claims

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Application Information

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IPC IPC(8): C12P21/02C07K14/78C07K1/34C12R1/225C12R1/46
CPCC07K14/78
Inventor 李健
Owner 兰溪市哥特生物技术有限公司
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